A study was made on the influence of histamine, serotonine and bradykinine on the perineurial permeability to horseradish peroxidase in mouse sciatic nerve. Following application of peroxidase around the exposed nerves the drugs were administered locally and the animals were allowed to survive for 5–20 min. The localization of the enzyme was then determined by an enzymehistochemical method.
In control nerves peroxidase diffuses in the epineurium but is prevented from reaching the endoneurium by the impermeable perineurium. Even very high concentrations of the tested substances did not change this type of spread of the marker enzyme. Consequently, the perineurium appears to maintain its property as diffusion barrier to proteins even under the influence of these chemical mediators of the inflammatory process.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Allt, G.: Involvement of the perineurium in experimental allergic neuritis: Electron microscopic observations. Acta neuropath. (Berl.)20, 139–149 (1972)
Broman, T., Lindberg-Broman, A. M.: An experimental study of disorders in the permeability of the cerebral vessels (“the blood-brain-barrier”) produced by chemical and physicochemical agents. Acta physiol. scand.10, 102–125 (1945)
Burkel, W. E.: The histological fine structure of perineurium. Anat. Rec.158, 177–190 (1967)
Cotran, R. S., Litt, M.: Ultrastructural localization of horseradish peroxidase and endogenous peroxidase activity in guinea pig peritoneal macrophages. J. Immunol.105, 1536–1546 (1970)
Denny-Brown, P.: Importance of neuronal fibroblasts in the regeneration of nerve. Arch. Neurol. Psychiat. (Chic.)55, 171–215 (1946)
Karnovsky, M. J.: A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol.27, 137A (1965)
Klemm, H.: Das Perineurium als Diffusionsbarriere gegenüber Peroxydase bei epi- und endoneuraler Applikation. Z. Zellforsch.108, 431–445 (1970)
Kristensson, K., Olsson, Y. The perineurium as a diffusion barrier to protein tracers. Differences between mature and immature animals. Acta neuropath. (Berl.)17, 127–138 (1971)
Kurucz, J., Osgyáni, J.: Contributions to the pathomorphology of pulmonary innervation. Acta morph. Acad. Sci. hung.4, 227–232 (1954)
Majno, G., Palade, G. E.: Studies on inflammation. I. The effect of histamine and serotonine on vascular permeability: An electron microscopic study. J. biophys. biochem. Cytol.11 571–605 (1961)
Majno, G., Shea, S. M., Leventhal, M.: Endothelial contraction induced by histamine-type mediators. An electron microscopic study. J. Cell Biol.42, 647–672 (1969)
Martin, K. H.: Untersuchungen über die perineurale Diffusionsbarriere an gefriergetrockneten Nerven. Z. Zellforsch.64, 404–428 (1964)
Olsson, Y.: Studies on vascular permeability in peripheral nerves: 2. Distribution of circulating fluorescent albumin in rat sciatic nerve after local injection of 5-hydroxytryptamine, histamine and compound 48/80. Acta physiol. scand.69, Supp. 284 (1966)
Olsson, Y., Reese, T. S.: Permeability of vasa nervorum and perineurium in mouse sciatic nerve studied by fluorescence and electron microscopy. J. Neuropath. exp. Neurol.30, 105–119 (1971)
Olsson, Y., Kristensson, K.: The perineurium as a diffusion barrier to protein tracers following trauma to nerves. Acta neuropath. (Berl.)23, 105–111 (1972)
Ross, M. H., Reith, M. J.: Perineurium: Evidence for contractile elements. Science165, 604–606 (1969)
Shanta, T. R., Bourne, G. H.: The perineural epithelium — a new concept. In: The structure and function of nervous tissue, Ed. G. H. Bourne, Vol. 1. New York-London: Academic Press 1968
Shanthaveerappa, T. R., Bourne, G. H.: The perineural epithelium: Nature and Significance. Nature (Lond.)199, 577–579 (1963)
Söderfeldt, B., Olsson, Y. Kristensson, K.: The perineurium as a diffusion barrier to protein tracers in human peripheral nerves. Acta neuropath. (Berl.)25, 120–126 (1973)
Zweifach, B. W.: Microcirculatory aspects of tissue injury. Ann. N. Y. Acad. Sci.116, 831–838 (1964)
About this article
Cite this article
Söderfeldt, B. The perineurium as a diffusion barrier to protein tracers. Acta Neuropathol 27, 55–60 (1974). https://doi.org/10.1007/BF00687240
- Perifascicular Diffusion Barrier
- Mouse Sciatic Nerve