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Immunohistochemical demonstration of increase in prostaglandin F2-alpha after recirculation in global ischemic rat brains

Summary

Immunohistochemical localization of prostaglandin F (PG F) was studied in 24 rats. In 21 rats, global brain ischemia was produced for 5 min by Pulsinelli's method. Prior to decapitation, 13 were recirculated for 5 min, while the remaining eight were not. Three recirculated rats were pretreated with indomethacin before the occlusion. Hypotension was induced during the occlusion to 40–50 mm Hg of mean arterial blood pressure in 11 rats including those unrecirculated, recirculated and pretreated with indomethacin. Three normal rats without occlusion of arteries served as control. The brains were snap frozen and 10-μm cryostat sections were incubated in rabbit anti-PG F serum and stained by the indirect immunofluorescence method after fixation in carbodiimide and in Zamboni's solution. Positive staining for PG F was noted mainly in pial vessels in normal and ischemic rats both with and without hypotension. The rats recirculated without hypotensive ischemia revealed a positive reaction in the walls of pial and parenchymal vessels. All rats recirculated after the hypotensive occlusion showed positive staining in blood vessels, in the cytoplasm of neurons (especially in hippocampi) and in the interfascicular oligodendrocytes. The above results indicate that recirculation after ischemia results in an increase in PG F in parenchymal vessels, neurons and oligodendrocytes.

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References

  1. 1.

    Abdel-Halim MS, Änggård E (1979) Regional and species differences in endogenous prostaglandin biosynthesis by brain homogenates. Prostaglandins 17:411–418

  2. 2.

    Abdel-Halim MS, Sjöquist B, Änggård E (1978) Inhibition of prostaglandin synthesis in rat brain. Acta Pharmacol Toxicol (Copenh) 43:266–272

  3. 3.

    Axen U (1974) N,N′-carbonyldiimidazole as coupling reagent for the preparation of bovine serum albumin conjugates. Prostaglandins 5:45–47

  4. 4.

    Bazán NG (1976) Free arachidonic acid and other lipids in the nervous system during early ischemia and after electroshock. Adv Exp Med Biol 72:317–335

  5. 5.

    Bito LZ Davson H, Hollingsworth JR (1976) Facilitated transport of prostaglandins across the blood-cerebrospinal fluid and blood-brain barriers. J Physiol (Lond) 256:273–285

  6. 6.

    Furlow TW Jr (1982) Cerebral ischemia produced by fourvessel occlusion in the rat: a quantitative evaluation of cerebral blood flow. Stroke 13:852–855

  7. 7.

    Galli C, Spagnuolo C (1976) The release of brain free fatty acids during ischemia in essential fatty acid-deficient rats. J Neurochem 26:401–404

  8. 8.

    Gaudet RJ, Alam I, Levine L (1980) Accumulation of cyclooxygenase products of arachidonic acid metabolism in gerbil brain during reperfusion after bilateral common carotid artery occlusion. J Neurochem 35:653–658

  9. 9.

    Gecse A, Ottlecz A, Mezei Z, Telegdy G (1982) Prostacyclin and prostaglandin synthesis in isolated brain capillaries. Prostaglandins 23:287–297

  10. 10.

    Gerritsen ME, Parks TP, Printz MP (1980) Prostaglandin endoperoxide metabolism by bovine cerebral microvessels. Biochim Biophys Acta 619:196–206

  11. 11.

    Hoare DG, Koshland DE Jr (1967) A method for the quantitative modification and estimation of carboxylic acid groups in proteins. J Biol Chem 242:2447–2453

  12. 12.

    Kiwak KJ, Moskowitz MA, Levine L (1985) Leukotriene production in gerbil brain after ischemic insult, subarachnoid hemorrhage, and concussive injury. J Neurosurg 62:865–869

  13. 13.

    La Torre E, Patrono C, Fortuna A Grossi-Belloni D (1974) Role of prostaglandin F in human cerebral vasospasm. J Neurosurg 41:293–299

  14. 14.

    Lindgren JÅ, Hökfelt T, Dahlén S-E, Patrono C, Samuelson B (1984) Leukotrienes in the rat central nervous system. Proc Natl Acad Sci USA 81:6212–6216

  15. 15.

    McCulloch J, Kelly PAT, Grome JJ, Pickard JD (1982) Local cerebral circulatory and metabolic effects of indomethacin. Am J Physiol 243:H416-H423

  16. 16.

    Mori Y, Mine M (1981) The localization of prostaglandins in the rabbit kidneys demonstrated with indirect immunofluorescence. Biomed Res 2:281–284

  17. 17.

    Nakano J, Prancan AV Moore SE (1972) Metabolism of prostaglandin E1 in cerebral cortex and cerebellum of the dog and rat. Brain Res 39:545–548

  18. 18.

    Nordström C-H, Rehncrona S, Siesjö BK (1978) Effects of phenobarbital in cerebral ischemia. II. Restitution of cerebral energy state, as well as of glycolytic metabolites, citric acid cycle intermediates and associated amino acids after pronounced incomplete ischemia. Stroke 9:335–343

  19. 19.

    Ogra SS, Kirton DT, Tomasi TB Jr, Lippes J (1974) Prostaglandin in the human fallopian tube. Fertil Steril 25:250–255

  20. 20.

    Perez G, McGuckin J (1972) Cellular localization of prostaglandin A2 in the rat kidney. Prostaglandins 2:393–398

  21. 21.

    Pulsinelli WA, Brierley JB (1979) A new model of bilateral hemispheric ischemia in the unanesthetized rat. Stroke 10:267–272

  22. 22.

    Rehncroma S, Westerberg E, Åkesson B, Siesjö BK (1982) Brain cortical fatty acids and phospholipids during and following complete and severe incomplete ischemia. J Neurochem 38:84–93

  23. 23.

    Shohami E, Rosenthal J, Lavy S (1982) The effect of incomplete cerebral ischemia on prostaglandin levels in rat brain. Stroke 13:494–499

  24. 24.

    Smith WL, Gutekunst DI, Lyons RH Jr (1980) Immunocytochemical localization of the prostaglandin-forming cyclooxygenase in the cerebellar cortex. Prostaglandins 19:61–67

  25. 25.

    Spagnuolo C, Sautebin L, Galli G, Racagni G, Galli C, Mazzari D, Finesso M (1979) PG F, thromboxane B2 and HETE levels in gerbil brain cortex after ligation of common carotid arteries and decapitation. Prostaglandins 18:53–61

  26. 26.

    Wolfe LS (1982) Eicosanoids: prostaglandins, thromboxanes, leukotrienes, and other derviatives of carbon-20 unsaturated fatty acids. J Neurochem 38:1–14

  27. 27.

    Wolfe LS, Mamer OA (1975) Measurement of prostaglandin F levels in human cerebrospinal fluid in normal and pathological conditions. Prostaglandins 9:183–192

  28. 28.

    Wolfe LS, Pappius HM, Marion J (1976) The biosynthesis of prostaglandins by brain tissue in vitro. Adv Prostaglandin Thromboxane Res 1:345–355

  29. 29.

    Zamboni L, De Martino C (1967) Buffered picric acidformaldehyde: a new, rapid fixative for electron microscopy. J Cell Biol 35:148A

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Correspondence to N. F. Kassell.

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Ogawa, H., Sasaki, T., Kassell, N.F. et al. Immunohistochemical demonstration of increase in prostaglandin F2-alpha after recirculation in global ischemic rat brains. Acta Neuropathol 75, 62–68 (1987). https://doi.org/10.1007/BF00686794

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Key words

  • prostaglandin F
  • Immunohistochemistry
  • Ischemia
  • Recirculation
  • Carbodiimide