Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Reverse pinocytosis induced in cerebral endothelial cells by injection of histamine into the cerebral ventricle

  • 24 Accesses

  • 10 Citations


Histamine dihydrochloride (10 μg of 500 μg/ml) was infused during 1 min into the lateral cerebral ventricle of rats, which resulted in a significant stimulation of pinocytosis in the endothelial cells. Systemic injections of mepyramine or metiamide could not prevent this activation. In contrast, ranitidine, injected with histamine was able to inhibit the stimulation of pinocytosis. Albumin exudation from the blood was not found. There was also no change in water and electrolyte contents of the brain tissue. The results suggest that histamine reaching the abluminal membrane can activate the pinocytosis in the cerebral endothelial cells in the reverse direction, i.e., from brain to blood, without opening the blood-brain barrier.

This is a preview of subscription content, log in to check access.


  1. 1.

    Broadwell RD, Balin BJ, Saloman M, Kaplan RS (1983) Brain-blood barrier? Yes and no. Proc Natl Acad Sci USA 80:7352–7356

  2. 2.

    Cockerham LG, Forcino CD, Pellmar TC, Prell GD, Smart SW (1987) Effect of antihistamines on postirradiation cerebral blood flow and plasma levels of histamine. Neuroscience 22:743

  3. 3.

    Daly MJ, Humphray JM, Stables R (1981) Some in vitro and in vivo actions of the new histamine H2-receptor antagonist, Ranitidine. Br J Pharmacol 72:49–54

  4. 4.

    Dropp JJ (1972) Mast cells in the central nervous system of several rodents. Anat Rec 174:227–238

  5. 5.

    Dux E, Joó F, (1982) Effects of histamime on brain capillaries: fine structural an immunohistochemical studies after intracarotid infusion. Exp Brain Res 47:252–258

  6. 6.

    Dux E, Joó F, Karnushina I, Jójárt I (1981) Effect of histamine on the permeability, fine structure and adenylate cyclase activity of brain capillaries. In: Kovách AGB, Hamar L, Szabó E (eds) Advances in Physiological Science, vol 7. Cardiovascular physiology. Pergamon Press-Akadémiai Kiadó, Budapest, pp 321–330

  7. 7.

    Dux E, Temesvári P, Szerdahelyi P, Nagy Á, Kovács J, Joó F (1987) Protective effect of antihistamines on cerebral oedema induced by experimental pneumothorax in newborn piglets. Neuroscience 22:317–321

  8. 8.

    Gross PM (1982) Cerebral histamine: indications for neuronal and vascular regulation. J Cereb Blood Flow Metab 2:3–23

  9. 9.

    Harling-Berg C, DePasquale M, Knopf P, Cserr HF (1987) Ratio of cerebrospinal fluid (CSF) to serum antibody titers are higher following central than systemic immunization in the normal rat. Neuroscience 22:S566

  10. 10.

    Joó F, Tóth I and Jancsó G (1975) Brain adenylate cyclase: its common occurrence in the capillaries and astrocytes Naturwissenschaften 8:397

  11. 11.

    Joó F, Dux E, Karnushina I, Halász N, Gecse Á, Ottlecz A, Mezei Zs (1981) Histamine in brain capillaries. Agent Actions 11:129–134

  12. 12.

    Karnushina IL, Palacios JM, Barbin G, Dux E, Joó F, Schwartz JC (1979) Histamine-related enzymes and histamine receptors in isolated brain capillaries. Agents Actions 9:89–90

  13. 13.

    Karnushina IL, Palacios JM, Barbin G, Dux E, Joó F, Schwartz JC (1980) Studies on a capillary-rich fraction isolated from brain: histaminic components and characterization of the histamine receptors linked to adenylate cyclase. J Neurochem 34:1201–1208

  14. 14.

    König JFR, Klippel RA (1974) The rat brain. Krieger, Huntington, NY

  15. 15.

    Schwartz JC (1979) Histamine receptors in brain. Life Sci 25:895–912

  16. 16.

    Sternberger LA (1974) Immunocytochemistry. Prentice-Hall, Englewood Cliffs, NJ

  17. 17.

    Strum WB (1983) Ranitidine. J Am Med Assoc 250:1894–1896

  18. 18.

    Takagi H, Morishima Y, Matsuyama T, Hayasi H, Watanabe T, Wada H (1986) Histaminergic axons in the neostriatum and cerebral cortex of the rat: a correlated light and electron microscopic immunocytochemical study using histidie decarboxylase as a marker. Brain Res 364:114–123

  19. 19.

    VanDeurs B (1977) Vesicular transport of horseradish peroxidase from brain to blood in segments of the cerebral microvasculature in adult mice. Brain Res 124:1–8

  20. 20.

    Vorbrodt AW, Lossinsky AS, Wisniewski HM, Suzuki R, Yamaguchi T, Masaoka H, Klatzo I (1985) Ultrastructural observations on the transvascular route of protein removal in vasogenic brain edema. Acta Neuropathol (Berl) 66: 265–273

  21. 21.

    Wilmes F, Hossmann K-A (1979) A specific immunofluorescence technique for the demonstration of vasogenic brain edema in paraffin-embedded material. Acta Neuropathol (Berl) 45:47–51

Download references

Author information

Correspondence to E. Dux.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Dux, E., Dóczi, T., Joó, F. et al. Reverse pinocytosis induced in cerebral endothelial cells by injection of histamine into the cerebral ventricle. Acta Neuropathol 76, 484–488 (1988). https://doi.org/10.1007/BF00686387

Download citation

Key words

  • Histamine
  • Cerebral ventricle
  • Pinocytosis
  • Brain-blood barrier