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Dose dependence of the cytokinetic and cytotoxic effects of epirubicin in vitro

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CHO cells were exposed in vitro for 1 h to concentrations of 0.1–20 μg/ml of the cytostatic drug epirubicin. Population growth, survival fractions, cell-cycle-phase distribution, and BrdU incorporation were analyzed. A fraction of the cells showed a transitory cytostatic reaction at 1 μg/ml, and >99% of the cells were killed at 10 μg/ml. The survival curve was biphasic with a steep slope at concentrations of up to 5 μg/ml. Approximately 0.1% of the cells were resistant to higher concentrations of epirubicin. Bivariate DNA/BrdU flow cytometry revealed that the sensitive cells were blocked and probably killed in the G2M phase of the cell cycle.

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Correspondence to Detlef Bartkowiak.

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Bartkowiak, D., Hemmer, J. & Röttinger, E. Dose dependence of the cytokinetic and cytotoxic effects of epirubicin in vitro. Cancer Chemother. Pharmacol. 30, 189–192 (1992). https://doi.org/10.1007/BF00686310

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  • Cell Cycle
  • Flow Cytometry
  • Cancer Research
  • Population Growth
  • Survival Curve