The cells of the dorsal root ganglia of scorbutic guinea pigs were characterized by a number of changes. In the animals that were sacrificed at 22–27 days following the ascorbic acid deficient diet, the sensory ganglion cells showed a complete breakdown of Nissl substance, a loss of neurofilaments and neurotubules, and swollen mitochondria. There was an increase in agranular and coated vesicles, alteration in dense bodies, some cytoplasmic sequestration. The periphery of some cells appeared profusely vacuolar. Cellular debris surrounding these cells was probably due to degenerating satellite cells. Severe cellular damage was noted in existing satellite cells. No collagen fibrils were seen in the endoneural spaces.
It is suggested that the cellular changes indicate a breakdown in the protein synthesizing mechanism of the cell.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Droz, B.: Protein metabolism in nerve cells. In: International Review of Cytology, pp. 363–390, Vol. 25. G. H. Bourne and J. F. Danielli, eds. New York-London: Academic Press, Inc. 1969.
Friederici, H. H. R., Taylor, H., Rose, R., Pirani, C. L.: The fine structure of capillaries in experimental scurvy. Lab. Invest.15, 1442–1458 (1966).
Goodman, M., Greenspon, S. A., Krakower, C. A.: The antigenic composition of the various autonomic structures of the canine kidney. J. Immunol.75, 96–111 (1955).
Gore, I., Tanaka, Y., Fujinami, T., Goodman, M. L.: Aortic acid mucopolysaccharides and collagen in scorbutic guinea pigs. J. Nutr.87, 311–316 (1965).
Gould, Bernard S.: The role of certain vitamins in collagen formation. In: Treatise on Collagen, pp. 323–365, Vol. 2, Part A. G. N. Ramachandran, ed. London-New York: Academic Press, Inc. 1968.
Gould, Bernard S.: Collagen formation and fibrogenesis with special reference to the role of ascorbic acid. In: International Review of Cytology, pp. 301–361, Vol. 15. G. H. Bourne and J. F. Danielli, eds. New York-London: Academic Press, Inc. 1963.
Harkin, J. C.: Localization of the cellular site of collagen synthesis in peripheral nerves by electron microscopic autoradiography using H3-proline, pp. 861–864. In: Proc. Fifth Intern. Congr. Neuropath. F. Lüthy and A. Bischoff, eds. No. 100. International Congress Series. Amsterdam: Exerpta Medica Foundation 1966.
Kafalides, N. A., Winzler, R. S.: The chemistry of glomerular basement membrane and its relationship to collagen. Biochemistry5, 702–712 (1966).
Karnovsky, M. S.: A tormaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy. J. Cell Biol.27, 137a (1965).
Leech, R. W.: Changes in satellite cells of rat dorsal root ganglia during central chromatolysis: An electron microscopic study. Neurology (Minneap.)17, 349–358 (1967).
Lloyd, B. B., Sinclair, H. M.: Vitamin C. In: Biochemistry and Physiology of Nutrition, Vol.1, pp. 369–471. G. H. Bourne and G. W. Kidder, eds. New York: Academic Press, Inc. 1953.
Masurovsky, E. B., Bunge, M. B., Bunge, R. P.: Cytological studies of organotypic cultures of rat dorsal root ganglia following x-irradiation in vitro. 1. Changes in neurons and satellite cells. J. Cell Biol.32, 467–496 (1967).
Nathaniel, E. J. H., Pease, D. C.: Collagen and basement membrane formation by Schwann cells during nerve regeneration. J. Ultrastruct. Res.9, 550–560 (1963).
Palay, Sanford L.: Principles of cellular organization in the nervous system. In: The Neurosciences, pp. 24–31. G. C. Quarton, eds. T. Melnechuk, and F. O. Schmitt. New York: the Rockefeller University Press 1967.
Pannese, Ennio: Investigation on the ultrastructural changes of the spinal ganglion neurons in the course of axon regeneration and cell hypertrophy. Z. Zellforsch.61, 561–586 (1963).
Ross, R., Benditt, E. P.: Wound healing and collagen formation. II. Fine structure in experimental scurvy. J. Cell Biol.12, 533–551 (1962).
Ross, R., Benditt, E. P.: Wound healing and collagen formation. IV. Distortion of ribosomal patterns of fibroblasts in scurvy. J. Cell Biol.22, 365–389 (1964).
Sebrell, W. H., Jr., Harris, R. S.: Ascorbic acid. In: The Vitamins, Vol. 1, pp. 177–393. New York: Academic Press, Inc. 1954.
Sulkin, D. F., Sulkin, N. M.: An electron microscopic study of autonomic ganglion cells of guinea pigs during ascorbic acid deficiency and partial inanition. Lab. Invest.16, 142–152 (1967).
Sulkin, D. F., Sulkin, N. M., Rothrock, M. L.: Fine structure of autonomic ganglia in recovery following experimental scurvy. Lab. Invest.19, 55–66 (1968).
Sulkin, N. M., Sulkin, D. F.: An electron microscopic study of the effects of chronic hypoxia on cardiac muscle, hepatic, and autonomic ganglion cells. Lab. Invest.14, 1523–1546 (1965).
Thomas, P. K.: The deposition of collagen in relation to Schwann cell basement membrane during peripheral nerve regeneration. J. Cell Biol.23, 375–382 (1964).
Wolbach, S. B., Bessey, O. A.: Tissue changes in vitamin deficiencies. Physiol. Rev.22, 233–289 (1942).
Supported in part by Grant 8217 from the Veterans Administration and United States Public Health Service Research Grant MH-19168.
About this article
Cite this article
Sulkin, D.F., Sulkin, N.M. & Nushan, H. Fine structure of sensory ganglia during experimental scurvy. Acta Neuropathol 23, 141–151 (1973). https://doi.org/10.1007/BF00685768
- Ascorbic Acid Deficiency
- Spinal Ganglion Cells
- Satellite Cells
- Nissl Substance
- Endoplasmic Reticulum
- Sensory Ganglia