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Respiratory chain of bacterial membrane: assembly-mobile carriers equilibrium

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Summary

The target size of NADH-oxidase activity ofM. lysodeikticus isolated membranes for electron radiation is nearly equal to that obtained for NADH-dehydrogenase (about 50 kD). The complete cross-linking of membrane proteins by glutaraldehyde causes an increase of NADH-oxidase target size to 3–3.5 times its original value. Electrons are transported by cross-linked respiratory chain from NADH to O2 with 60–50% effectiveness of that in untreated membranes. It is proposed that electrons are transported through a multi-enzymic complex of individual carriers having limited lifetime with exchange of carriers between different respiratory complexes via lateral diffusion in membrane.

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References

  1. 1.

    De Pierre, J. and Ernster, L., 1977. Ann. Rev. Biochem. 46: 201–262.

  2. 2.

    Varfolomeev, S. D. and Berezin, I. V., 1977. Dokl. Akad. Nauk U.S.S.R. 236: 748–751.

  3. 3.

    Kagawa, Y., 1967. Biochim. Biophys. Acta 131: 586–588.

  4. 4.

    Rogers, M. J. and Strittmatter, P., 1974. J. Biol. Chem. 249: 895–900.

  5. 5.

    Hackenbrock, C., 1981. Trends Biochem. Sci. 6: 151–154.

  6. 6.

    Kaprelyants, A. S. and Ostrovsky, D. N., 1975. Biokhimiya 40: 1210–1215.

  7. 7.

    Martin, B. R., 1981. Biochem. Soc. Trans. 9: 44–46.

  8. 8.

    Houslay, M. D., Ellory, J. C., Smith, G. A., Hesketh, T. R., Stein, J. M., Warren, G. B. and Metcalfe, J. C., 1977. Biochim. Biophys. Acta 467: 208–219.

  9. 9.

    Gelman, N. S., Zhukova, I. G. and Oparin, A. I., 1963. Biokhimiya 28: 122–127.

  10. 10.

    Wess, J., Allen, A. and Schwarz, H. A., 1955. Proc. 1st Intern. Conf. on the peaceful uses of atomic energy, Vol. 14, pp. 179–181, Geneva.

  11. 11.

    Hutchinson, F. and Pollard, E., 1961. Mechanisms in Radiobiology (Errera, M. and Forssberg, A., eds.), Vol. 1, p. 71, Academic Press, New York.

  12. 12.

    Ostrovsky, D. N., Bulgakova, V. G., Zhukova, I. G., Kaprelyants, A. S., Rosantzev, E. G. and Simakova, I. M., 1976. Biokhimiya 41: 175–181.

  13. 13.

    O'Farrell, P. H., 1975. J. Biol. Chem. 250: 4007–4021.

  14. 14.

    Zhukova, I. G., Valueva, T. A., Degteva, G. K., Kaprelyants, A. S., Nosova, T. A., Sepetov, N. F. and Ostrovsky, D. N., 1982. Biokhimiya, in press.

  15. 15.

    Tikhonova, G. V., Arzatbanov, V. Yu., Sheiko, T. V. and Ostrovsky, D. N., 1981. Dokl. Akad. Nauk U.S.S.R. 260: 497–501.

  16. 16.

    Burbaev, D. Sh., Dgemuchadze, G. K., Sologenkin, I. N., Ostrovsky, D. N. and Lukoyanova, M. A., 1980. Dokl. Akad. Nauk U.S.S.R. 255: 472–476.

  17. 17.

    Peterson, J., Ebei, R. E., O'Keeffe, D., Matsubara, T. and Estabrook, R., 1976. J. Biol. Chem. 251: 4010–4016.

  18. 18.

    French, J., Guegerich, F. and Coon, M., 1980. J. Biol. Chem. 255: 4112–4119.

  19. 19.

    Zinov'eva, M. E., Simakova, I. M. and Kaprelyants, A. S., 1979. Biokhimiya 44: 931–939.

  20. 20.

    Kaprelyants, A. S., 1982. Biokhimiya 47: 883–895.

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Zinov'eva, M.E., Kaprelyants, A.S. & Ostrovsky, D.N. Respiratory chain of bacterial membrane: assembly-mobile carriers equilibrium. Mol Cell Biochem 55, 141–144 (1983). https://doi.org/10.1007/BF00673708

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Keywords

  • Membrane Protein
  • NADH
  • Glutaraldehyde
  • Respiratory Chain
  • Lateral Diffusion