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Sensitivity and adaptation in R7, an ultraviolet photoreceptor, in theDrosophila retina

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Summary

Receptor deficient mutants and chromatic adaptation were used to isolate the contribution of R7 to the electroretinogram (ERG) ofDrosophila. R7 was found to be a single-peaked ultraviolet (UV) receptor (Fig. 1). Photoconversion of the UV absorbing rhodopsin (R) to its stable 470–495 nm metarhodopsin (M) was shown to elicit a long-lived negative (depolarizing) afterpotential (Fig. 3) while inactivating R7. Photoreconversion ofM toR reactivates R7 (Fig. 2) and repolarizes the ERG (Fig. 3). The intensities of light needed to elicit afterpotentials by photointerconverting R7 photopigment were found to be about 2 log units greater than for R1-6 photopigment (Fig. 4). Vitamin A deprivation decreases R7 (as well as R8) sensitivity by about 2 log units (through decreased photopigment levels) without changing spectral sensitivity shape (Fig. 5). Vitamin A deprivation further eliminates the light-induced inactivation of R7 allowing experiments designed to characterize the in vivo spectral absorption of R7M. R7M was found to have UV and 495 nm maxima (Fig. 6). No polarization sensitivity was detected in the R7 ERG component. The adaptational properties of R7 are similar to the properties previously established for R1-6 but different from the properties of R8.

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References

  1. Bishop, L.G.: An ultraviolet photoreceptor in a dipteran compound eye. J. comp. Physiol.91, 267–275 (1974)

  2. Burkhardt, D.: Spectral sensitivity and other response characteristics of single visual cells in the arthropod eye. Symp. Soc. exp. Biol.16, 86–109 (1962)

  3. Dartnall, H.J.A.: The interpretation of spectral sensitivity curves. Brit. med. Bull.9, 24–30 (1953)

  4. Doane, W.W.:Drosophila. In: Methods in developmental biology (ed. F.H. Wilt, N.K. Wessels). New York: Thomas Y. Crowell Co. 1967

  5. Eckert, H.: Die spektrale Empfindlichkeit des Komplexauges vonMusca (Bestimmung aus Messungen der optomotorischen Reaktion). Kybernetik9, 145–156 (1971)

  6. Eckert, H., Bishop, L.G., Dvorak, D.R.: Spectral sensitivities of identified receptor cells in the blowflyCalliphora. Naturwissenschaften63, 47–48 (1976)

  7. Goldsmith, T.H., Bernard, G.D.: The visual system of insects. In: Physiology of insecta, Vol. II, 2nd ed. (ed. M. Rockstein). New York: Academic Press 1974

  8. Goldsmith, T.H., Fernandez, H.R.: Some photochemical and physiological aspects of visual excitation in compound eyes. In: The functional organization of the compound eye (ed. C.G. Bernard). Oxford-New York: Pergamon Press 1966

  9. Greenberg, A.D., Honig, B., Ebrey, T.G.: Wavelength dependence of the bandwidths of visual pigment spectra. Nature (Lond.)257, 823–824 (1975)

  10. Hamdorf, K., Rosner, G.: Adaptation und Photoregeneration im Fliegenauge. J. comp. Physiol.86, 281–292 (1973)

  11. Hamdorf, K., Schwemer, J., Gogala, M.: Insect visual pigment sensitive to ultraviolet light. Nature (Lond.)231, 458–459 (1971)

  12. Harris, W.A., Stark, W.S.: Hereditary retinal degeneration inDrosophila melanogaster: a mutant defect associated with the phototransduction process. J. gen. Physiol.69, in press (1977)

  13. Harris, W.A., Stark, W.S., Walker, J.A.: Genetic dissection of the photoreceptor system in the compound eye ofDrosophila melanogaster. J. Physiol. (Lond.)256, 415–439 (1976)

  14. Järvilehto, M., Moring, J.: Polarization sensitivity of individual retinula cells and neurons of the flyCalliphora. J. comp. Physiol.91, 387–397 (1974)

  15. Kirschfeld, K., Franceschini, W.: Microspectrophotometry of fly rhabdomeres. Summary of lecture held at conference on Visual Physiology, Oct. 5–8, 1975, Günzburg, Germany

  16. Kirschfeld, K., Lutz, B.: Lateral inhibition in the compound eye of the fly,Musca. Z. Naturforsch.29, 95–97 (1974)

  17. Langer, H., Thorell, B.: Microspectrophotometric assay of visual pigments in single rhabdomeres of the insect eye. In: The functional organization of the compound eye (ed. C.G. Bernard), pp. 145–149. Oxford-New York: Pergamon Press 1966

  18. Meffert, P., Smola, U.: Electrophysiological measurements of spectral sensitivity of central visual cells in the eye of blowfly. Nature (Lond.)260, 342–344 (1976)

  19. Mimura, K.: Some spatial properties in the first optic ganglion of the fly. J. comp. Physiol.105, 65–82 (1976)

  20. Minke, B., Wu, C.-F., Pak, W.L.: Isolation of light induced response of the central retinula cells from the electroretinogram ofDrosophila. J. comp. Physiol.98, 345–355 (1975)

  21. Ostroy, S.E., Wilson, M., Pak, W.L.:Drosophila rhodopsin: photochemistry extraction and differences in thenorp AP12 phototransduction mutant. Biochem. biophys. Res. Commun.59, 960–966 (1974)

  22. Pak, W.L., Lidington, K.J.: Fast electrical potential from a long lived, long-wavelength photoproduct of fly visual pigment. J. gen. Physiol.63, 740–756 (1974)

  23. Razmjoo, S., Hamdorf, K.: Visual sensitivity and the variation of total photopigment content in the blowfly photoreceptor membrane. J. comp. Physiol.105, 279–286 (1976)

  24. Rosner, G.: Adaptation und Photoregeneration im Fliegenauge. J. comp. Physiol.102, 269–295 (1975)

  25. Schümperli, R.A.: Evidence for colour vision inDrosophila melanogaster through spontaneous choice behavior. J. comp. Physiol.86, 77–94 (1973)

  26. Smola, U., Meffert, P.: A single-peaked UV-receptor in the eye ofCalliphora erythrocephala. J. comp. Physiol.103, 353–357 (1975)

  27. Snyder, A.W.: Polarization sensitivity of individual retinula cells. J. comp. Physiol.83, 331–360 (1973)

  28. Snyder, A.W., Pask, C.: Spectral sensitivity of dipteran retinula cells. J. comp. Physiol.84, 59–76 (1973)

  29. Stark, W.S.: Spectral selectivity of visual response alterations mediated by interconversions of native and intermediate photopigments inDrosophila. J. comp. Physiol.96, 343–356 (1975)

  30. Stark, W.S.: Diet, vitamin A and vision inDrosophila. Submitted for Drosoph. Inf. Serv. 52 (1976)

  31. Stark, W.S., Hu, K.G.: Sensitivity, adaptation and function of R7, an ultraviolet receptor mDrosophila. In: Society for Neuroscience Program and Abstracts (1976), p. 1094 (abstract)

  32. Stark, W.S., Ivanyshyn, A.M., Hu, K.G.: Spectral sensitivities and photopigments in adaptation of fly visual receptors. Naturwissenschaften63, 513–518 (1976)

  33. Stark, W.S., Wasserman, G.S.: Wavelength-specific ERG characteristics of pigmented- and whiteeyed strains ofDrosophila. J. comp. Physiol.91, 427–441 (1974)

  34. Stark, W.S., Zitzmann, W.G.: Isolation of adaptation mechanisms and photopigment spectra by vitamin A deprivation inDrosophila. J. comp. Physiol.105, 15–27 (1976)

  35. Stavenga, D.G., Zantema, A., Kuiper, J.W.: Rhodopsin processes and the function of the pupil mechanism in flies. In: Biochemistry and physiology of visual pigments (ed. H. Langer). BerlinHeidelberg-New York: Springer 1973

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Supported by NSF grants BMS-74-12817 and BNS 76-11921. I thank M. Chapin, R. Greenberg, K. Hu, A. Ivanyshyn, D. Lakin, G. Pransky, D. Sawyer, J. Walker and W. Zitzmann for technical assistance.

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Stark, W.S. Sensitivity and adaptation in R7, an ultraviolet photoreceptor, in theDrosophila retina. J. Comp. Physiol. 115, 47–59 (1977). https://doi.org/10.1007/BF00667784

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Keywords

  • Retina
  • Spectral Sensitivity
  • Spectral Absorption
  • Deficient Mutant
  • Polarization Sensitivity