Cyclic AMP binding proteins have been measured in normal breast tissue, in a variety of physiological conditions including resting state (69 cases), pregnancy (15 cases), lactation (4 cases), post-lactational involution (6 cases), and prolonged involution (10 cases). Levels varied greatly within the groups but median values were elevated in pregnancy, lactation, and post-lactational involution as compared with the resting and prolonged involutionary states. The proportion of different types of cyclic AMP binding proteins also differed between groups. Resting breast showed approximately equal amounts of type I and II whereas pregnant and post-lactational involuting breast tissue had an increased proportion of type I binding, and the prolonged involutionary state tended to be associated with low type I. Within the subgroup of nulliparous resting breasts, the proportion of type I cyclic AMP binding protein was significantly elevated during the second half of the menstrual cycle compared with the first (p < 0.05). There were also significant positive associations between the level of epithelial proliferation and both total (p < 0.015) and type I (p < 0.002) cyclic AMP binding. This represents the strongest of associations thus far reported between signalling systems and proliferation in the normal breast.
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Beebe SJ, Corbin JD: Cyclic nucleotide dependent protein kinases. In: Boyer PD, Krebs EG (eds) The Enzymes, Volume XVII, Control by Phosphorylation Part A. Acad Press, New York, 1986, pp 43–111
Cho-Chung YS: Role of cyclic AMP receptor proteins in growth, differentiation and suppression of malignancy: new approaches to therapy. Cancer Res 50: 7093–7100, 1990
Miller WR, Elton RA, Dixon JM, Chetty U, Watson DMA: Cyclic AMP binding proteins and prognosis in breast cancer. Br J Cancer 61: 263–266, 1990
Sapag-Hagar Y, Greenbaum AL: Adenosine 3′:5′-mono-phosphate and hormone interrelationships in the mammary gland of the rat during pregnancy and lactation. Eur J Biochem 47: 303–312, 1974
Sharoni Y, Feldman B, Teuerstein I, Levy J: Protein kinase activity in the rat mammary gland during pregnancy, lactation and weaning: A correlation with growth but not with progesterone receptor levels. Endocrinol 115: 1918–1924, 1984
Valivullah HM, Keenan TW: Cyclic AMP dependent phosphorylation, Ca2+ and calmodulin: possible influences on acyltransferases and pyruvate dehydrogenase activities of rat mammary glands. Int J Biochem 18: 477–480, 1986
Going JJ, Anderson TJ, Battersby S, MacIntyre CCA: Proliferative and secretory activity in human breast during natural and artificial menstrual cycles. Am J Pathol 130: 193–204, 1988
Battersby S, Anderson TJ: Correlation of proliferative activity in breast tissue using PCNA/Cyclin. Human Pathol 21: 781 (letter), 1990
Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Anal Biochem 72: 248–254, 1976
Miller WR, Senbanjo RO, Telford J, Watson DMA: Cyclic AMP binding proteins in human breast cancer. Br J Cancer 52: 531–535, 1985
Scatchard F: The attraction of proteins for small molecules and ions. Ann NY Acad Sci 51: 660–672, 1949
Pomerantz AH, Rudolph SA, Haley BE, Greengard P: Photoaffinity labelling of a protein kinase from bovine brain with 8-azidoadenosine 3′,5′ monophosphate. Biochemistry 14: 3858–3862, 1975
Laemmli UK: Cleavage of structural protein during the assembling of the head of bacteriophage T4. Nature 27: 680–685, 1970
Battersby S, Anderson TJ: Histological changes in breast tissue that characterise recent pregnancy. Histopathol 15: 415–419, 1989
Battersby S, Anderson TJ: Proliferative and secretory activity in the pregnant and lactating human breast. Virchows Archiv A 413: 189–196, 1988
Battersby S, Robertson BJ, Anderson TJ, King RJB, McPherson K: Influence of menstrual cycle, parity and oral contraceptive use on steroid hormone receptors in normal breast. Br J Cancer 65: 601–607, 1992
Sheffield LG: Oligonucleotides antisense to catalytic subunit of cyclic AMP-dependent protein kinase inhibit mouse mammary epithelial cell DNA synthesis. Exp Cell Res 192: 307–310, 1991
Friedman DL: Regulation of the cell cycle and cellular proliferation by cyclic nucleotides. In: Kebabian JW, Nathanson JA (eds) Handbook of Experimental Pharmacology Vol. 58/11. Springer-Verlag, Berlin, 1982, pp 151–188
Anderson TJ, Battersby S, King RJB, McPherson K, Going JJ: Oral contraceptive use influences resting breast proliferation. Human Pathol 20: 1139–1144, 1989
Sheffield LG, Aysworth CF, Welsch CW: Cyclic nucleotides and protein phosphorylation in mouse mammary glands: effects of estrogen and progesterone administeredin vivo. Proc Soc Exp Biol Med 185: 283–290, 1987
Miyazaki K, Miyamoto E, Maeyama M, Uchida M: Specific regulation by steroid hormones of protein kinases in the endometrium. 2. Alterations in level of protein kinases in human endometrium during the menstrual cycle. Eur J Biochem 104: 543–547, 1989
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Battersby, S., Anderson, T.J. & Miller, W.R. Patterns of cyclic AMP binding in normal human breast. Breast Cancer Res Tr 30, 153–158 (1994). https://doi.org/10.1007/BF00666059
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