Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Aggregation numbers of SDS micelles formed on EHEC. A steady state fluorescence quenching study

  • 223 Accesses

  • 33 Citations


The present investigation proves that in the interaction between an uncharged polymer and a negatively charged amphiphilic ion (surfactant) clusters are actually formed and it provides data for the cluster concentration and the cluster size and their variation with composition. The polymer bound cluster size increases after a certain critical surfactant concentration and passes through a maximum. This maximum cluster size decreases with decreasing polymer concentration and attains a limiting value at infinite dilution. For the highest polymer concentration the cluster size is close to the size of normal surfactant micelles. The cluster concentration was determined by a fluorescence quenching technique and the amount surfactant adsorbed to the polymer by dialysis equilibrium measurements. Combining these independent sets of data permits the cluster aggregation number to be unambiguously determined. Solubilization experiments indicate the possibility to regulate the amount solubilized by varying the polymer concentration. The molecular properties of the system are sensitively monitored by the variation in two vibronic peaks in the pyrene fluorescence emission spectrum which defines a “hydrophobic index”. Very good agreement is found between all three experimental methods. Finally, the model suggested is analyzed in terms of coil size and cluster-cluster distance. Depending upon the degree of adsorption saturation and the density of polymer segments in solution the interaction may switch from being intramolecular to becoming intermolecular.

This is a preview of subscription content, log in to check access.


  1. 1.

    Holmberg C, Nilsson S, Singh SK, Sundelöf L-O (1992) J Phys Chem 96:871

  2. 2.

    Carlsson A (1989) Nonionic cellulose ethers-Interactions with surfactants, solubility and other aspects. Dissertation, Lund

  3. 3.

    Goddard ED (1986) Colloids and surfaces 19:255 (Review on interactions between surfactants and neutral polymers)

  4. 4.

    Nilsson S, Holmberg C, Sundelöf L-O (1994) Colloid Polym Sci 272:338

  5. 5.

    Results from our laboratory dealing with temperature dependence of the EHEC/SDS/water system properties

  6. 6.

    Holmberg C, Nilsson S, Sundelöf L-O Thermodynamic properties of surfactant/polymer/water systems with respect to clustering adsorption and micelle formation as a function of temperature and polymer concentration. The EHEC/SDS/water system (Manuscript in preparation

  7. 7.

    Results from our laboratory dealing with electrolyte dependence of the EHEC/SDS/water system properties

  8. 8.

    Hammarström A, Sundelöf L-O (1993) Colloid Polym Sci 271:1129

  9. 9.

    Sundelöf L-O (1992) 34th IUPAC Int Symposium on Macromolecules, Prague, Results presented during lecture (Special lecture 2-SL3)

  10. 10.

    Turro NJ, Yekta A (1978) J Am Chem Soc 100:5951

  11. 11.

    Zana R (1987) In: Zana R (ed) Surfactant solutions, new methods of investigation. Marcel Dekker, New York, pp. 241

  12. 12.

    Thomas JK (1980) Chem Reviews 80:283

  13. 13.

    Zana R, Lianos P, Lang J (1985) J Phys Chem 89:41

  14. 14.

    Zana R, Lang J, Lianos P (1982) Polym Prep (Am Chem Soc Div Polym Chem) 23:39

  15. 15.

    Lissi EA, Abuin E (1985) J Colloid Interface Sci 105:1

  16. 16.

    Winnik FM, Winnik MA (1990) Polym J 22:482

  17. 17.

    Winnik FM, Winnik MA, Tazuke S (1987) J Phys Chem 9:594

  18. 18.

    Winnik FM, Ringsdorf H, Venzmer J (1991) Langmuir 7:905

  19. 19.

    Sivadasan K, Somasundaran P (1990) Colloids and surfaces 49:229

  20. 20.

    Chandar P, Somasundaran P, Turro NJ (1988) Macromolecules 21:950

  21. 21.

    Witte FM, Engberts JBFN (1989) Colloids and surfaces 36:417

  22. 22.

    Brackman JC, Engberts JBFN (1991) Langmuir 7:2097

  23. 23.

    Tanaka R, Meadows J, Phillips GO, Williams PA (1990) Carbohydr Polym 12:443

  24. 24.

    Chu D, Thomas JK (1986) J Am Chem Soc 108:6270

  25. 25.

    Löfroth J-E, Johansson L, Norman A-C, Wetterström K (1991) Progr Colloid Polym Sci 84:73

  26. 26.

    Turro NJ, Baretz BH, Kuo PL (1984) Macromolecules 17:1321

  27. 27.

    Reports in which they have used the pyrene/benzophenone pair to determineN and to study fluorescence quenching in micellar solutions. Almgren M, Alsins J, van Stam J, Mukhtar E (1988) Progr Colloid Polym Sci 76:68 and (1988) Wikander G, Johansson LB-Å (1989) Langmuir 5:728

  28. 28.

    Malliaris A (1987) Progr Colloid Polym Sci 73:161

  29. 29.

    Almgren M, Löfroth J-E (1981) J Colloid Interface Sci 81:486

  30. 30.

    Lianos P, Zana R (1981) J Colloid Interface Sci 84:100

  31. 31.

    Hayter JB, Penfold J (1983) Colloid Polym Sci 261:1022

  32. 32.

    Moroi Y, Humphry-Baker R, Gratzel M (1987) J Colloid Interface Sci 119:588

  33. 33.

    Kalyanasundaram K, Thomas JK (1977) J Am Chem Soc 99:2039

  34. 34.

    Ståhlberg J, Almgren M (1985) Anal Chem 57:817

  35. 35.

    Jones MN (1967) J Colloid Interface Sci 23:36

  36. 36.

    Handbook of pharmaceutical excipients (1986) American Pharmaceutical Association (AphA), Washington DC, pp. 272

  37. 37.

    Ananthapadmanabhan KP, Goddard ED, Turro NJ, Kuo PL (1985) Langmuir 1:352

  38. 38.

    Schott H (1966) J Phys Chem 70:2966

  39. 39.

    Goddard ED, Hanan RB (1977) In: Mittal KL (ed) Micellization, solubilization and microemulsions vol. 2. Plenum Press, New York, pp. 835

  40. 40.

    Anacker EW (1968) J Phys Chem 72:379

  41. 41.

    Breuer MM, Robb ID (1972) Chemistry and Industry 13:530 (Review on interactions between surfactants and polymers)

  42. 42.

    Dubin PL, Gruber JH, Xia J, Zhang H (1992) J Colloid Interface Sci 148:35

  43. 43.

    Moroi Y, Akisada H, Saito M, Matura R (1977) J Colloid Interface Sci 61:233

  44. 44.

    Cabane B, Duplessix R (1982) J de Physique 43:1529 and (1987) J de Physique 48:651

  45. 45.

    Sjöberg Å, Karlström G, Tjerneld F (1989) Macromolecules 22:4512

  46. 46.

    Unpublished Size-exclusion liquid chromatography (SEC) experiments on cellulose derivatives from our laboratory

  47. 47.

    Wirick MG, Waldman MH (1970) J Appl Polym Sci 14:579

  48. 48.

    Manley RStJ (1956) Arkiv för Kemi 9:519

Download references

Author information

Correspondence to Prof L. -O. Sundelöf.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Sundelöf, L.-., Nilsson, S. & Holmberg, C. Aggregation numbers of SDS micelles formed on EHEC. A steady state fluorescence quenching study. Colloid Polym Sci 273, 83–95 (1995). https://doi.org/10.1007/BF00655678

Download citation

Key words

  • Aggregation numbers
  • ethyl hydroxyethyl cellulose
  • sodium dodecyl sulphate
  • interaction
  • fluorescence quenching
  • dye solubilization