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Aggregation numbers of SDS micelles formed on EHEC. A steady state fluorescence quenching study

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Abstract

The present investigation proves that in the interaction between an uncharged polymer and a negatively charged amphiphilic ion (surfactant) clusters are actually formed and it provides data for the cluster concentration and the cluster size and their variation with composition. The polymer bound cluster size increases after a certain critical surfactant concentration and passes through a maximum. This maximum cluster size decreases with decreasing polymer concentration and attains a limiting value at infinite dilution. For the highest polymer concentration the cluster size is close to the size of normal surfactant micelles. The cluster concentration was determined by a fluorescence quenching technique and the amount surfactant adsorbed to the polymer by dialysis equilibrium measurements. Combining these independent sets of data permits the cluster aggregation number to be unambiguously determined. Solubilization experiments indicate the possibility to regulate the amount solubilized by varying the polymer concentration. The molecular properties of the system are sensitively monitored by the variation in two vibronic peaks in the pyrene fluorescence emission spectrum which defines a “hydrophobic index”. Very good agreement is found between all three experimental methods. Finally, the model suggested is analyzed in terms of coil size and cluster-cluster distance. Depending upon the degree of adsorption saturation and the density of polymer segments in solution the interaction may switch from being intramolecular to becoming intermolecular.

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Correspondence to Prof L. -O. Sundelöf.

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Sundelöf, L.-., Nilsson, S. & Holmberg, C. Aggregation numbers of SDS micelles formed on EHEC. A steady state fluorescence quenching study. Colloid Polym Sci 273, 83–95 (1995). https://doi.org/10.1007/BF00655678

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Key words

  • Aggregation numbers
  • ethyl hydroxyethyl cellulose
  • sodium dodecyl sulphate
  • interaction
  • fluorescence quenching
  • dye solubilization