The transport of three organic cations, tetraethylammonium (TEA), morphine and N1-methylnicotinamide (NMN) was studied in brush border membrane vesicles from rabbit kidney cortex under voltage clamp conditions. A proton gradient (pHi=6.0, pHo=7.4) produced a large stimulation of TEA and morphine uptake, yielding a transient overshoot of 190 and 220% respectively, as compared to equilibrium uptake values. No overshoot was observed under pH equilibrium conditions (pHi=pHo=7.4, control). These data suggest the presence of a proton-organic cation exchange mechanism in the rabbit renal cortical brush border membrane. Identical experimental conditions (proton gradient) failed however to stimulate significantly NMN transport above control values measured under pH equilibrium conditions. Proton gradient driven TEA transport showed an inhibition of 21% in the presence of NMN (1 mM) and of 63% in the presence of TEA (1 mM), and TEA transport was stimulated by preloading the vesicles with 1 mM TEA (305%) but not with 1 mM NMN (128%). NMN transport showed an inhibition of 39% in the presence of 1 mM TEA and of 27% in the presence of 1 mM NMN and its transport was stimulated by preloading the vesicles with 1 mM TEA (228%) and 1 mM NMN (178%). Our data suggest that TEA, NMN and morphine are transported by a common transport mechanism for which NMN has only a low affinity.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Berner W, Kinne R (1976) Transport of p-aminohippuric acid by plasma membrane vesicles isolated from rat kidney cortex. Pflügers Arch 361:269–277
Besseghir K, Pearce LB, Rennick B (1981) Renal tubular transport and metabolism of organic cations by the rabbit. Am J Physiol 241:F308-F314
Beyer KH, Russo HF, Gass SR, Wilhoyte KM, Pitt AA (1950) Renal tubular elimination of N1-methylnicotinamide. Am J Physiol 160:311–320
Biber J, Stieger B, Haase W, Murer H (1981) A high yield preparation for rat kidney brush border membranes. Different behaviour of lysosomal markers. Biochim Biophys Acta 647:169–176
Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72:248–254
Holohan PD, Ross CR (1981) Mechanisms of organic cation transport of kidney plasma membrane vesicles. 2. ΔpH studies. J Pharmacol Exp Ther 216:294–298
Manganel M, Roch-Ramel F, Murer H (1985) Na-pyrazionate cotransport in rabbit renal brush border membrane vesicles. Am J Physiol 249:F400-F408
McKinney TD, Kunneman M (1985) Procainamide transport in rabbit renal cortical brush border membrane vesicles. Am J Physiol 249:F532-F541
Rennick BR (1981) Renal tubule transport of organic cations. Am J Physiol 240:F83-F89
Scalera S, Storelli C, Storelli-Joss C, Haase W, Murer H (1980) A simple and fast method for the isolation of basolateral plasma membranes from rat small-intestinal epithelial cells. Biochem J 186:177–181
Schäli C, Roch-Ramel F (1982) Transport and metabolism of3H morphine in isolated, nonperfused proximal tubular segments of the rabbit kidney. J Pharmacol Exp Ther 223:811–815
Takano M, Inui KI, Okano T, Saito H, Hori R (1984) Carrier-mediated transport systems of tetraethylammonium in rat renal brush-border and basolateral membrane vesicles. Biochim Biophys Acta 773:113–124
Weiner IM (1985) Organic acids and bases and uric acid. In: Seldin DW, Giebisch G (eds) The kidney: physiology and pathophysiology, chapt 72. Raven Press, New York, pp 1703–1724
Wright SH (1985) Transport of N1-methylnicotinamide across brush border membrane vesicles from rabbit kidney. Am J Physiol 249:F903-F911
About this article
Cite this article
Rafizadeh, C., Manganel, M., Roch-Ramel, F. et al. Transport of organic cations in brush border membrane vesicles from rabbit kidney cortex. Pflugers Arch. 407, 404–408 (1986). https://doi.org/10.1007/BF00652625
- Organic cation transport
- Brush border membrane (rabbit kidney)