The posterior hypothalamus of cats immobilized with gallamine was superfused through a push-pull cannula with artificial cerebrospinal fluid. Addition of3H-tyrosine into the superfusing fluid led to synthesis of3H-catecholamines which were released spontaneously. Separation of the3H-catecholamines by column chromatography or their acetylation and separation by paper chromatography revealed that both3H-noradrenaline and3H-dopamine were released. In most experiments3H-noradrenaline represented about 10 to 25% of total3H-catecholamines. Superfusion of the hypothalamus with amphetamine (1×10−5 M) enhanced the release of total3H-catecholamines, the release of3H-noradrenaline being relatively more enhanced than that of3H-dopamine. Determination of the readioactive compounds in the hypothalamus at the end of the experiments showed that total3H-catecholamines represented 3% of3H-tyrosine. About 15% of the total3H-catecholamines were due to3H-noradrenaline and 85% to3H-dopamine.
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Besson, M. J., Cheramy, A., Glowinski, J.: Effects of some psychotropic drugs on dopamine synthesis in the rat striatum. J. Pharmacol. exp. Ther.177, 196–205 (1971a)
Besson, M. J., Cheramy, A., Glowinski, J.: Dopamine: spontaneous release and drug-induced release from the caudate nucleus in the cat. Brain Res.32, 407–424 (1971b)
Björklund, A., Nobin, A.: Fluorescence histochemical and microspectrofluorimetric mapping of dopamine and noradrenaline cell groups in the rat diencephalon. Brain Res.51, 193–205 (1973)
Carr, L. A., Moore, K. E.: Norepinephrine: Release from brain byd-amphetamine in vivo. Science164, 322–323 (1969)
Cheramy, A., Gauchy, C., Glowinski, J., Besson, M. J.: In vivo activation by benzotropine of dopamine release and synthesis in the caudate nucleus. Europ. J. Pharmacol.21, 245–248 (1973)
Glowinski, J., Iversen, L. L., Axelrod, J.: Storage and synthesis of norepinephrine in the reserpine-treated rat brain. J. Pharmacol. exp. Ther.151, 386–399 (1966)
Laverty, R., Sharman, D. F.: The estimation of small quantities of 3,4-dihydroxyphenylethylamine in tissues. Brit. J. Pharmacol.24, 538–548 (1965)
Merlis, J. K.: The effect of changes in the calcium content of cerebrospinal fluid on spinal reflex activity in the dog. Amer. J. Physiol.13, 67–72 (1940)
Philippu, A.: Release of catecholamines from the hypothalamus by drugs and electrical stimulation. In: H. J. Schümann and G. Kroneberg, eds: New aspects of storage and release mechanism of catecholamines, pp. 258–267. Berlin-Heidelberg-New York: Springer 1970
Philippu, A., Heyd, G., Burger, A.: Release of noradrenaline from the hypothalamus in vivo. Europ. J. Pharmacol.9, 52–58 (1970)
Philippu, A., Przuntek, H., Roensberg, W.: Superfusion of the hypothalamus with gamma-aminobutyric acid. Effect on release of noradrenaline and blood pressure. Naunyn-Schmiedeberg's Arch. Pharmacol.276, 103–118 (1973)
Reinoso-Suarez, F.: Topographischer Hirnatlas der Katze. Merck AG, Darmstadt, 1961
Riddell, D., Szerb, J. C.: The release “in vivo” of dopamine synthetized from labelled precursors in the caudate nucleus of the cat. J. Neurochem.18, 989–1006 (1971)
Strada, S. J., Sulser, F.: Comparative effects ofp-chloroamphetamine and amphetamine on metabolsim and in vivo release of3H-norepinephrine in the hypothalamus. Europ. J. Pharmacol.15, 45–51 (1971)
Thierry, A. M., Blanc, G., Glowinski, J.: Effect of stress on the disposition of catecholamines localized in various intraneuronal storage forms in the brain stem of the rat. J. Neurochem.18, 449–461 (1971)
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Philippu, A., Glowinski, J. & Besson, M.J. In vivo release of newly synthesized catecholamines from the hypothalamus by amphetamine. Naunyn-Schmiedeberg's Arch. Pharmacol. 282, 1–8 (1974). https://doi.org/10.1007/BF00647398