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l-Alanine as an end product of glycolysis inSaccharomyces cerevisiae growing under different hypoxic conditions

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Saccharomyces cerevisiae growing under anaerobic or other hypoxic conditions releasesl-alanine into the culture medium as an end product of glycolysis. Although the production of alanine is not as high as that of other fermentation products (ethanol, glycerol, succinic acid), consideration of the pathways leading to alanine in fermenting yeasts indicates that the release of alanine is advantageous to the cellular economy and may be considered as a safety device for excreting reduction equivalents derived from NADPH. No significant changes in the activity of alanine aminotransferase are found in the yeast when grown under different conditions.

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  1. Bergmeyer, H. U. (ed.) 1974. Methods of Enzymatic Analysis 2nd ed. — Academic Press, New York.

  2. Brosnan, J. T. andWilliamson, D. H. 1974. Mechanisms for the formation of alanine and aspartate on rat liverin vivo after administration of ammonium chloride. — Biochem. J.138: 453–462.

  3. Johnston, I. A. 1975. Anaerobic metabolism in the carp (Carassius carassius L.). — Comp. Biochem. Physiol.51 B: 235–241.

  4. Lagunas, R. andGancedo, J. M. 1973. Reduced pyridine-nucleotides balance in glucose-growingSaccharomyces cerevisiae. — Eur. J. Biochem.37: 90–94.

  5. Leclerc, L., Fréminet, A., Bursaux, E. etPoyart, C. 1976. Hyperalaninémie au cours de l'hypoxie aiguë chez le lapin anesthésié. — Bull. E. Physiopath. Resp.12: 781–789.

  6. Lowry, O. H., Rosebrough, N. I., Farr, A. L. andRandall, R. J. 1951. Protein measurement with the Folin phenol reagent. — J. Biol. Chem.193: 265–275.

  7. Lupiáñez, J. A., Machado, A., Núñez de Castro, I. andMayor, F. 1974. Succinic acid production by yeasts grown under different hypoxic conditions. — Mol. Cell. Biochem.3: 113–116.

  8. Machado, A., Núñez de Castro, I. andMayor, F. 1975. Isocitrate dehydrogenases and oxoglutarate dehydrogenase activities of baker's yeast grown in a variety of hypoxic conditions. — Mol. Cell. Biochem.6: 93–100.

  9. Núñez de Castro, I., Ugarte, M., Cano, A. andMayor, F. 1970. Effect of glucose, galactose, and different nitrogen-sources on the activity of yeast glutamate dehydrogenase (NAD and NADP-linked) from normal strain and impaired respiration mutant. — Eur. J. Biochem.16: 567–570.

  10. Núñez de Castro, I. Arias-Saavedra, J. M., Machado, A. andMayor, F. 1974. Effect of inhibitors of mitochondrial protein synthesis on the NADH and NADPH glutamate dehydrogenases in yeast. — Mol. Cell. Biochem.3: 109–111.

  11. Núñez de Castro, I., Arias de Saavedra, J. M., Machado, A. andMayor, F. 1976. Regulation of the level of yeast citrate synthase by oxygen availability. — Mol. Cell. Biochem.12: 161–169.

  12. Oudejans, R. C. H. M. andvan der Horst, D. J. 1974. Aerobic-anaerobic biosynthesis of fatty acids and other lipids from glycolytic intermediates in the pulmonate land snailCepaea nemoralis L. — Comp. Biochem. Physiol.47 B: 139–147.

  13. Saéz, M. J. andLagunas, R. 1976. Determination of intermediary metabolites in yeast. Critical examination of the effect of sampling conditions and recommendations for obtaining true levels. — Mol. Cell. Biochem.13: 73–78.

  14. Satrústegui, J. andMachado, A. 1977. The synthesis of yeasts matrix mitochondrial enzymes is regulated by different levels of mitochondrial function. — Arch. Biochem. Biophys.184: 355–363.

  15. Snell, K. andWalker, D. G. 1972. The adaptative behaviour of isoenzyme forms of rat liver alanine aminotransferases during development. — Biochem. J.128: 403–413.

  16. Stokes, T. M. andAwapara, J. 1968. Alanine and succinate as end products of glucose degradation in the clamRangia cuneata. — Comp. Biochem. Physiol.25: 883–892.

  17. Taegtmeyer, H., Peterson, M. B., Ragavan, V. V., Ferguson, A. G. andLesch, M. 1977.De novo alanine synthesis in isolated oxygen-deprived rabbit myocardium. — J. Biol. Chem.252: 5010–5018.

  18. de Zwaan, A. andZandee, D. I. 1972. The utilization of glycogen and accumulation of some intermediates during anaerobiosis inMytilus edulis L. — Comp. Biochem. Physiol.43 B: 47–54.

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The research was supported by a grant from the Comisión Asesora de Investigación Científica y Téchnica.

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Chico, E., Olavarría, J.S. & de Castro, I.N. l-Alanine as an end product of glycolysis inSaccharomyces cerevisiae growing under different hypoxic conditions. Antonie van Leeuwenhoek 44, 193–201 (1978).

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  • Glycerol
  • Alanine
  • Saccharomyces Cerevisiae
  • Hypoxic Condition