Journal of Materials Science

, Volume 27, Issue 23, pp 6391–6400 | Cite as

Transverse dimensions of wood pulp fibres by confocal laser scanning microscopy and image analysis

  • H. F. Jang
  • A. G. Robertson
  • R. S. Seth


Cross-sectional images of wood-pulp fibres were generated using optical sectioning by a confocal laser scanning microscope. The technique has a distinct advantage over mechanical sectioning with a microtome, as it simplifies specimen preparation. Cross-sectional images were obtained for unbleached softwood kraft pulp fibres using epifluorescence mode. The accuracy of cross-sectional images was verified by imaging fluorescent microspheres. An image analysis procedure, in which the boundaries of fibre cross-sections were defined with a maximum-gradient edge-finding technique, was developed for measuring fibre cross-sectional area and wall thickness rapidly and accurately. The measurements were insensitive to the confocal microscope's asymmetric resolution, signal deterioration through the specimen thickness, overall image quality and operator bias.


Confocal Laser Scanning Microscope Confocal Laser Scanning Kraft Pulp Transverse Dimension Pulp Fibre 
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Copyright information

© Chapman & Hall 1992

Authors and Affiliations

  • H. F. Jang
    • 1
  • A. G. Robertson
    • 1
  • R. S. Seth
    • 1
  1. 1.Pulp and Paper Research Institute of CanadaVancouverCanada

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