Some aspects of the structure of the active center of the enzyme L-asparaginase
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Summary
Some functional groups in the active center of the enzyme L-asparaginase have been studied by the method of inhibitory analysis.
It has been established that the phenol groups of the tyrosine residues and the imidazole groups of the histidine residues are important for the occurrence of the enzymatic reaction.
The blocking of sulfhydryl, disulfide, and amino groups, and also of the hydroxy groups of serine and threonine, does not suppress the enzymatic activity.
Keywords
Methylene Blue Asparaginase Succinic Anhydride Thioglycolic Acid Sulfanilic AcidPreview
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Literature cited
- 1.H. Marguardt, Arzn. Forsch.,18, 1380 (1968).Google Scholar
- 2.R. H. Adamson and S. Farbo, Cancer Chem. Report,52, 617 (1968).Google Scholar
- 3.H. M. Suld and R. A. Herbert, J. Biol. Chem.,245, 2802 (1970).PubMedGoogle Scholar
- 4.Y. S. Halpern and N. Grossowiez, Biochem. J.,65, 716 (1957).CrossRefGoogle Scholar
- 5.N. De Groot and N. Lichtenstein, Biochim. Biophys. Acta,40, 92, 99 (1960).CrossRefGoogle Scholar
- 6.D. B. Tower, E. L. Peters, and W. C. Curtis, J. Biol. Chem.,238, 983 (1963).PubMedGoogle Scholar
- 7.R. A. Zhagat, M. Ya. Orovere, I. K. Shprunka, I. A. Éidus, and G. Ya. Melle, Mikrobiol. (1971).Google Scholar
- 8.J. Roberts, G. Burson, and J. H. Hill, J. Bacteriol.,95, 2117 (1968).PubMedPubMedCentralGoogle Scholar
- 9.S. Moore and W. H. Stein, Methods in Enzymology, Vol. 6, Academic Press, New York (1963), p. 819.Google Scholar
- 10.J. R. Spies and D. C. Chambers, Analyt. Chem.,21, 1249 (1949).CrossRefGoogle Scholar
- 11.R. A. Zhagat, I. A. Vina, R. K. Bluma, and D. É. Barone, Biokhimiya (1971).Google Scholar
- 12.H. A. Whelan and J. C. Wriston, Biochemistry,8, 2386 (1969).CrossRefGoogle Scholar
- 13.T. O. Yellin and J. C. Wriston, Biochemistry,5, 1605 (1966).CrossRefGoogle Scholar
- 14.M. L. Anson, Advan. Protein Chem.,2, 361 (1944).CrossRefGoogle Scholar
- 15.P. Wagner, E. Irson, A. Arens, and K. Bauer, Biochem. Biophys. Res. Comm.,37, 383 (1969).CrossRefGoogle Scholar
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