Reaction of yeast inorganic pyrophosphatase with maleic anhydride
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The reaction of yeast inorganic pyrophosphatase with an excess of maleic anhydride at 2° C for 5 min leads to the acylation of all ε-amino groups of lysine residues.
The protective groups are completely removed at pH 3.5 for 16 hr at 60° C. Acylation with maleic anhydride is accompanied by the dissociation of the yeast inorganic pyrophosphatase into its subunits.
KeywordsMaleic Anhydride Protective Group Succinic Anhydride Sedimentation Coefficient Performic Acid
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