Archives of Dermatological Research

, Volume 256, Issue 1, pp 23–31 | Cite as

Langerhans cells at the sites of vaccinia virus inoculation

  • Sadanori Nagao
  • Shogo Inaba
  • Susumu Iijima


Langerhans cells in the epidermis at the sites of vaccinia virus inoculation were studied with the electron microscope. The cells contained unusually increased numbers of the Langerhans cell granules. Such abnormal Langerhans cells have not been described except for in histiocytosis X. Vaccinia virus particles were found in the Langerhans cells, where they were located individually or embedded in the granular matrix or in lysosomes.


Public Health Electron Microscope Cell Granule Virus Particle Vaccinia Virus 


Langerhans-Zellen in der Epidermis der Impfpapeln nach der Pockenimpfung wurden elektronenmikroskopisch untersucht. Diese Zellen hatten ungewöhnlich zahlreiche Langerhans-Zell-Granula, die oft abnorme Konfigurationen zeigten. Solche Langerhans-Zellen sind bisher nicht beschrieben worden, außer bei der Histiocytose X. Die Vakzinviren wurden in den Langerhans-Zellen gefunden, wo die Viren isoliert oder in den granulären Matrizen bzw. in den Lysosomen existierten.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Basset, F., Nezolof, C.: Présence en microscope electronique de structure filamenteuse originales dans le lesions pulmonaires et osseuses de l'histiocytose X. Bull. Soc. med. Hop. Paris,117, 413–426 (1966)PubMedGoogle Scholar
  2. 2.
    Dales, S.: The uptake and development of vaccinia virus in strain L cells followed with labeled viral deoxyribonucleic acid. J. Cel.. Biol.18, 51–72 (1963)CrossRefGoogle Scholar
  3. 3.
    Ebner, H., Niebauer, G.: Die sogenannte “dunkle” Langerhans-Zelle. Arch. klin. exp. Derm.229, 217–222 (1967)CrossRefGoogle Scholar
  4. 4.
    Freed, E. R., Duma, R. J., Escobar, M. R.: Vaccinia necrosum and its relationship to impaired immunologic responsiveness. Amer. J. Med.52, 411–420 (1972)CrossRefGoogle Scholar
  5. 5.
    Hashimoto, K.: Langerhans cell granules. An endocytotic organelle. Arch. Derm.104, 148–160 (1971)CrossRefGoogle Scholar
  6. 6.
    Kobayashi, T., Asboe-Hansen, G.: Granules of Langerhans cells in Letterer-Siwe's disease. Acta derm.-venereol. (Stockh.)52, 257–262 (1972)Google Scholar
  7. 7.
    Lisi, P.: Investigation on Langerhans cells in pathological human epidermis. Acta derm.-venereol. (Stockh.)53, 425–428 (1973)Google Scholar
  8. 8.
    Nagao, S., Iijima, S.: A Langerhans cell in the spongiform pustule of pustular psoriasis. Arch. Derm. Forsch.245, 221–228 (1972)CrossRefGoogle Scholar
  9. 9.
    Nagao, S., Sonoda, K., Azuma, A.: Electron microscopic exfoliative cytology in viral dermatoses. Jap. J. Clin. Derm.28, 537–542 (1974) (in Japanese)Google Scholar
  10. 10.
    Nordquist, R. E., Olson, R. L., Everett, M. A.: The transport, uptake, and storage of ferritin in human epidermis. Arch. Derm.94, 482–499 (1966)CrossRefGoogle Scholar
  11. 11.
    Segebiel, R. W., Reed, T. H.: Serial construction of the characteristic granule of the Langerhans cell. J. Cell. Biol.36, 595–602 (1968)CrossRefGoogle Scholar
  12. 12.
    Silberberg, I.: Studies by electron microscopy on epidermis after topical application of mercuric chloride: morphologic and histochemical findings in epidermal cells of human subjects who do not show allergic or primary irritant reactions to mercuric chloride (0.1%). J. invest. Derm.56, 147–160 (1971)CrossRefGoogle Scholar
  13. 13.
    Silberberg, I., Baer, R. L., Rosenthal, S. A.: The role of Langerhans cells in contact allergy. I. An ultrastructural study in actively induced contact dermatitis in guinea pigs. Acta derm.-venereol. (Stockh.)54, 321–331 (1974)Google Scholar
  14. 14.
    Tusques, J., Pradal, G.: Analyse tridimensionells des inclusions rencontrees dans les histiocytes de l'histiocytose «X» en microscopie electronique. Comparaison le inclusions des cellules de Langerhans. J. Micros.8, 113–122 (1969)Google Scholar
  15. 15.
    Wheelock, E. F.: Interferon in dermal crusts of human vaccinia virus vaccinations. Possible explanation of relative benignity of variolation smallpox. Proc. Soc. exp. Biol. (N.Y.)117, 650–656 (194)CrossRefGoogle Scholar
  16. 16.
    Wolff, K., Honigsmann, H.: Permeability of the epidermis and the phagocytic activity of keratinocytes. Ultrastructural studies with thorotrast as a marker. J. Ultrastruc. Res.36, 176–190 (1971)CrossRefGoogle Scholar
  17. 17.
    Wolff, K., Lessard, R. J., Winkelmann, R. K.: Electron microscopic observations on Langerhans'cell after epidermal trauma. Dermatology Digest 35–47 (1971)Google Scholar
  18. 18.
    Wolff, K., Schreiner, E.: Uptake, intracellular transport and degradation of exogenous protein by Langerhans cells. An electron microscope-cytochemical study using peroxidase as tracer substance. J. invest. Derm.54, 37–47 (1970)CrossRefGoogle Scholar
  19. 19.
    Zelickson, A. S.: Melanocyte, melanin granules and Langerhans cell. In Ultrastructure of Normal Skin, pp. 163–182. Ed. by Zelickson, A. S. Philadelphia: Lea and Febiger 1967Google Scholar

Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • Sadanori Nagao
    • 1
  • Shogo Inaba
    • 1
  • Susumu Iijima
    • 1
  1. 1.Department of DermatologyFukushima Medical CollegeFukushimaJapan

Personalised recommendations