Archives of oto-rhino-laryngology

, Volume 233, Issue 1, pp 67–75 | Cite as

Permeability of the labyrinthine windows in guinea pigs

  • Katsuhiko Tanaka
  • Shoichi Motomura


Permeability of the labyrinthine windows in guinea pigs was investigated by electron microscopy using horseradish peroxidase (HRP). In the round window membrane, HRP particles were readily taken up by the epithelial cells and were transported to the subepithelial layer which was regarded as being bathed in the perilymph. The oval window also appears to allow the passage of the macromolecules in view of the HRP-labeled granules in the chondrocytes of the stapediovestibular joint. The transport of the HRP particles from the middle ear to the inner ear was confirmed by the uptake of those in the saccular epithelium after 30–40 min exposure to the tracer.

Key words

Round window membrane Oval window Permeability Horseradish peroxidase 

Permeabilität der Labyrinthfenster beim Meerschweinchen


Elektronenmikroskopische Untersuchungen über die Permeabilität der Labyrinthfenster des Meerschweinchens mit Meerrettichperoxidase (HRP) zeigen, daß sowohl durch das runde als auch durch das ovale Fenster Makromoleküle in das Innenohr transportiert werden. Als Beleg für diesen Transport ließen sich 30–40 min nach Applikation der HRP deren Partikel im Epithel des Sacculus nachweisen.


Rundes Fenster Ovales Fenster Permeabilität Meerrettichperoxidase 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Arnold W, Nitze HR, Ritter R, Ilberg C v, Ganzer U (1972) Qualitative Untersuchungen der Verbindungswege des Subarachnoidalraumes mit dem lymphatischen System des Kopfes und Halses. Acta Otolaryngol 74: 411–424CrossRefGoogle Scholar
  2. Goycoolea MV, Paparella MM, Goldberg B, Carpenter A (1980) Permeability of the round window membrane in otitis media. Arch Otolaryngol 106: 430–433CrossRefGoogle Scholar
  3. Graham RC, Karnovsky MJ (1966) The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney. Ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14: 291–302CrossRefGoogle Scholar
  4. Höft J (1969) Die Permeabilität und die Beeinflussung der Permeabilität der Membran des runden Fensters durch Pantocain (Tetracain). Arch Klin Exp Ohren-Nasen-Kehlkopfheilkd. 193: 128–137CrossRefGoogle Scholar
  5. Kumagami H, Nishida H, Dohi K (1976) Experimental labyrinthine lesion through stylomastoid foramen. ORL 38: 334–343CrossRefGoogle Scholar
  6. Lim DJ, Hussl B (1975) Macromolecular transport by the middle ear and its lymphatic system. Acta Otolaryngol 80: 19–31CrossRefGoogle Scholar
  7. Nakai Y, Kaneko M (1975) Round window membrane. Submicroscopic structure and permeability. Pract Otol (Kyoto) 68: 223–232CrossRefGoogle Scholar
  8. Rahm WE, Strother WF, Gulick WL, Crump JF (1959) The effects of topical anesthetics upon the ear. Ann Otol Rhinol Laryngol 68: 1037–1046CrossRefGoogle Scholar
  9. Rahm WE, Strother WF, Gulick WL (1960) The effects of anesthetics upon the ear. II. Procaine hydrochloride. Ann Otol Rhinol Laryngol 69: 969–975CrossRefGoogle Scholar
  10. Rahm WE, Strother WF, Gulick WL, Crump JF (1961) The effects of anesthetics upon the ear. III. Tetracaine hydrochloride. Ann Otol Rhinol Laryngol 70: 403–409CrossRefGoogle Scholar
  11. Richardson JR, Bouchard T, Ferguson CC (1976) Uptake and transport of exogenous proteins by respiratory epithelium. Lab Invest 35: 307–314PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Katsuhiko Tanaka
    • 1
  • Shoichi Motomura
    • 1
  1. 1.Dept. of OtolaryngologyHokkaido University School of MedicineSapporoJapan

Personalised recommendations