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Archives of oto-rhino-laryngology

, Volume 236, Issue 2, pp 173–184 | Cite as

Pattern of gentamicin-induced cochlear degeneration in the guinea pig

A morphological and electrophysiological study
  • R. A. Tange
  • E. A. J. G. Conijn
  • L. G. P. M. van Zeijl
  • E. H. Huizing
Article

Summary

Gentamicin-induced cochlear degeneration in the guinea pig was studied by complete hair-cell counting (cytocochleograms) and phase-contrast and interference microscopical examination of the stria vascularis and Reissner's membrane. Gentamicin (100 mg/kg/day) was administered over a period of 7–17 days. The first loss of hair cells (OHC) occurred in a region 6–8 mm from the round window. From this ‘degeneration point’, the loss of haircells progressed towards the round window (fast) and the apex (slowly). The stria vascularis showed no signs of degeneration. Reissner's membrane, on the other hand, showed intracellular vacuolization of the endolymphatic cells over the complete length of the cochlea after 12 or more days' intoxication. Hearing loss was measured by electrocochleography with skin electrodes. The histologic findings were compared with the objective audiograms.

Key words

Gentamicin-induced cochlear degeneration Degeneration point Reissner's membrane vacuolization Skin-electrode-evoked response audiometry 

Durch Gentamyzin verursachte Degeneration in der Schnecke des Meerschweinchens

Eine morphologische und elektrophysiologische Studie

Zusammenfassung

Die durch Gentamyzin verursachte Degeneration in der Kochlea des Meerschweinchens wurde mit dem Zytokochleogramm sowie mit Phasenkontrast- und Interferrenzmikroskopie der Stria vascularis und der Reissnerschen Membran dargestellt. Der erste Haarzellenverlust wurde 6–8 mm vom runden Fenster entfernt festgestellt. Von diesem „Degenerationspunkt“ aus schreitet der Haarzellverlust rasch zum runden Fenster und langsam spitzenwärts fort. In der Stria vascularis war keine Degeneration zu erkennen, aber die Reissnersche Membran zeigte eine intrazelluläre Vakuolisierung der endolymphatischen Zellen. Der Hörverlust wurde mit der Elektrokochleographie bestimmt und mit den histologischen Befunden verglichen.

Schlüsselwörter

Gentamyzin-induzierte Kochleadegeneration Degenerationspunkt Vakuolisierung der Reissnerschen Membran Elektrokochleographie 

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References

  1. 1.
    Beck ChL, Krahl P (1962) Experimentelle und feingewebliche Untersuchungen über die Ototoxicität von Kanamicin. Arch Ohren-Nasen-Kehlkopfheilkd 179: 594–610CrossRefGoogle Scholar
  2. 2.
    Békésy G von (1960) Experiments in hearing. McGraw/Hill, New YorkGoogle Scholar
  3. 3.
    Dallos P, Cheatham MA (1976) Production of cochlear potentials by inner and outer haircells. J Acoust Soc Am 60: 510–512CrossRefGoogle Scholar
  4. 4.
    Dallos P, Harris D (1978) Properties of the audiotory nerve responses in absence of outer haircells. J Neurophysiol 41: 365–383CrossRefGoogle Scholar
  5. 5.
    Darrouzet J, Guilhaume A (1976) Ototoxicité cochléaire comparée de trois antibiotiques: kanamycine, gentamicine, tombramycine. Rev Laryngol Otol Rhinol (Bord) 97/11–12: 655–673Google Scholar
  6. 6.
    Federspil P (1973) Histological investigation of the ototoxicity of gentamicin and tobramicin. Arzneim Forsch 12: 1739–1745Google Scholar
  7. 7.
    Federspil P (1979) Antibiotikaschäden des Ohres. JA Barth, LeipzigGoogle Scholar
  8. 8.
    Harpur ES, Bridges JB (1979) An evaluation of the use of scanning and transmission electronmicroscopy in a study of the gentamicin-damaged guinea pig organ of Corti. J Laryngol Otol 93: 7–23CrossRefGoogle Scholar
  9. 9.
    Hawkins Jr JE, Johnsson LG, Aran J (1969) Comparative test of gentamicin ototoxicity. J Infect Dis 119: 417–426CrossRefGoogle Scholar
  10. 10.
    Hawkins Jr JE (1973) Ototoxic medicaments — a working hypothesis. Audiology 12: 383–393CrossRefGoogle Scholar
  11. 11.
    Hawkins Jr JE, Johnsson LG (1975) Microdissection and surface preparations of the inner ear. In: Smith CA, Vernon JA (eds) Handbook of auditory and vestibular research methods. Charles C Thomas, Springfield, IL, pp 5–52Google Scholar
  12. 12.
    Johnsson LG, Hawkins Jr JE (1972) Sensory and neural degeneration with aging as seen in microdissections of the human inner ear. Ann Otol Rhinol Laryngol 81: 179–193CrossRefGoogle Scholar
  13. 13.
    Johnstone BM, Taylor KJ, Boyle AJ (1970) Mechanics of the guinea pig cochlea. J Acoust Soc Am 47: 504–509CrossRefGoogle Scholar
  14. 14.
    Kohonen A (1965) Effect of some ototoxic drugs upon the pattern and innervation of cochlear sensory cells in the guinea pig. Acta Otolaryngol [Suppl] (Stockh) 208: 9–70Google Scholar
  15. 15.
    Lundquist PG, Wersäll J (1967) The ototoxic effect of gentamicin. An electronmicroscopical study. In: Gentamicin First Int Symp Paris. Schwabe, Basel pp 26–46Google Scholar
  16. 16.
    Ryan A, Dallos P (1975) Effect of absence of cochlear outer haircells on behavioral auditory threshold. Nature 253: 44–46CrossRefGoogle Scholar
  17. 17.
    Stebbins WC, Hawkins Jr JE, Johnsson LG, Moody DB (1979) Hearing thresholds with outer and inner haircell loss. Am J Otolaryngol 1: 15–27CrossRefGoogle Scholar
  18. 18.
    Tange RA (1980) Normale en pathologische cytoarchitectuur van het binnenoor. Ned Tijdschr Geneeskd 124: 59Google Scholar
  19. 19.
    Tange RA, Huizing EH (1980) Hearing loss and inner ear changes in a patient suffering from severe gentamicin ototoxicity. Arch Otorhinolaryngol 228: 113–121CrossRefGoogle Scholar
  20. 20.
    Theopold HM (1977) Comparative surface studies of ototoxic effects of various aminoglycoside antibiotics on the organ of Corti in the guinea pig. Acta Otolaryngol 84: 57–64CrossRefGoogle Scholar
  21. 21.
    Webster WR, Dunlop CW (1965) Unaesthetised cats auditory evoked potentials and test cage acoustics: a methodological study. J Aud Res 5: 11Google Scholar
  22. 22.
    Wersäll J (1977) 10th International Congress of Chemotherapy in Zürich. In: Richardson RG (ed) Round table discussion on Gentamicin and Tobramicin. Academic Press, London/Grune and Stratton, New York, p 26Google Scholar
  23. 23.
    Wilson JP, Johnstone JR (1972) Capacitive probe measures of basilar membrane vibration. Symposium on hearing theory. IPO Eindhoven, Holland, 22–23 JuneGoogle Scholar
  24. 24.
    Wright I (1969) Investigation of ototoxicity of an antibiotic from micromonospora purpurea (gentamicin). J Pathol 98: 129–136CrossRefGoogle Scholar
  25. 25.
    Ylikoski J, Wersäll J, Björkroth B (1973) Hearing loss and cochlear pathology in gentamicin intoxicated guinea pigs. Acta Pathol Microbiol Scand [B] [Suppl] 241: 30–47Google Scholar
  26. 26.
    Ylikoski J (1974) Correlation between pure tone audiogram and cochlear pathology in guinea pigs intoxicated with ototoxic antibiotics. Acta Otolaryngol [Suppl] (Stockh) 236: 42CrossRefGoogle Scholar
  27. 27.
    Ylikoski J (1974) Guinea pig hair-cell pathology from ototoxic antibiotics. Acta Otolaryngol [Suppl] (Stockh) 326: 5CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • R. A. Tange
    • 1
  • E. A. J. G. Conijn
    • 1
  • L. G. P. M. van Zeijl
    • 1
  • E. H. Huizing
    • 1
  1. 1.Dept of OtorhinolaryngologyUniversity Hospital ‘Dijkzigt’GD RotterdamThe Netherlands

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