Advertisement

Current Genetics

, Volume 8, Issue 2, pp 121–126 | Cite as

Nuclear suppressors of mitochondrial chloramphenicol resistance in Baker's yeast: their use for the isolation of novel mutants

  • Jeffrey A. Knight
  • Cathy J. Wedeen
  • Kathryn A. Hughes
Article

Summary

Strains that are genotypically sensitive to chloramphenicol and also contain one of the nuclear suppressors of mitochondrial chloramphenicol resistance (Waxman et al. 1979) were constructed. A manganese mutagenesis on such a strain produced chloramphenicol resistant mutants, most of which resulted from mutations in nuclear genes. These mutants may be either dominant or recessive, and they probably do not code for membrane proteins. The few mitochondrial mutants fall into several classes, but all result from mutations in the 21S rRNA gene. The suppressor allele effectively prevents the appearance of the most common group of mitochondrial mutants (those that map at cap1), and thereby enhances the selection of novel mutants in the region.

Key words

Yeast Mitochondrial DNA Antibiotic resistance mutations Suppressor 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Birky CW Jr (1973) Genetics 74:421–432PubMedPubMedCentralGoogle Scholar
  2. Birky CW Jr (1975) Mol Gen Genet 141:41–58CrossRefGoogle Scholar
  3. Branlant C, Krol A, Machatt MA, Pouyet J, Ebel J-P, Edwards K, Kossel H (1981) Nucleic Acids Res 9:4303–4320CrossRefGoogle Scholar
  4. Cohen JD, Eaton N (1978) Genetics 91:19–33Google Scholar
  5. Dujon B (1980) Cell 20:185–197CrossRefGoogle Scholar
  6. Dujon B (1981) In: Strathern IN, Jones EW, Broach JR (eds) The molecular biology of the yeast Saccharomyces. Life cycle and inheritance. Cold Spring Harbor Laboratory, New York, pp 505–635Google Scholar
  7. Dujon B, Bolotin-Fukuhara M, Coen D, Deutsch J, Netter P, Slonimski PP, Weill L (1976) Mol Gen Genet 143:131–165CrossRefGoogle Scholar
  8. Faye G, Kujawa C, Dujon B, Bolotin-Fukuhara M, Wolf K, Fukuhara H, Slonimski PP (1975) J Mol Biol 99:203–217CrossRefGoogle Scholar
  9. Faye G, Sor F (1977) Mol Gen Genet 155:27–34CrossRefGoogle Scholar
  10. Glotz C, Zwieb C, Brimacombe R, Edwards K, Kossel H (1981) Nucleic Acids Res 9:3287–3306CrossRefGoogle Scholar
  11. Groot GSP (1974) In: Kroon AM, Saccone C (eds) The biogenesis of mitochondria. Academic Press, Amsterdam, pp 443–452CrossRefGoogle Scholar
  12. Groot GSP, Mason TL, Van Harten-Loosbroek N (1979) Mol Gen Genet 174:339–342CrossRefGoogle Scholar
  13. Joulou C, Bolotin-Fukuhara M (1982) Mol Gen Genet 188:256–260CrossRefGoogle Scholar
  14. Knight JA (1977) PhD Thesis, The Ohio State University, Columbus, OhioGoogle Scholar
  15. Knight JA (1980) Genetics 94:69–92PubMedPubMedCentralGoogle Scholar
  16. Knight JA, Courey AJ, Stebbins B (1982) Curr Genet 5:21–27CrossRefGoogle Scholar
  17. Kruszewska A, Szczesniak B (1978) Mol Gen Genet 160:171–181CrossRefGoogle Scholar
  18. Netter P, Petrochilo E, Slonimski PP, Bolotin-Fukuhara M, Coen D, Deutsch J, Dujon B (1974) Genetics 78:1063–1100PubMedPubMedCentralGoogle Scholar
  19. Noller H, Kop JA, Wheaton IV, Brosius J, Gutell RR, Kopylov AM, Dohme F, Herr W, Stahl DA, Gupta R, Woese CR (1981) Nucleic Acids Res 9:6167–6189CrossRefGoogle Scholar
  20. Rank GH, Bech-Hansen NT (1973) Mol Gen Genet 126:93–102CrossRefGoogle Scholar
  21. Sanders JPM, Heyting C, Borst P (1975) Biochem Biophys Res Commun 65:699–704CrossRefGoogle Scholar
  22. Sor F, Fukuhara H (1982) Nucleic Acids Res 10:6571–6577CrossRefGoogle Scholar
  23. Sor F, Fukuhara H (1983) Nucleic Acids Res 11:339–348CrossRefGoogle Scholar
  24. Stiegler P, Carbon P, Ebel J-P, Ehresmann C (1981) Eur J Biochem 120:487–495CrossRefGoogle Scholar
  25. Waxman MF, Knight JA, Perlman PS (1979) Mol Gen Genet 167:243–250CrossRefGoogle Scholar
  26. Wickerham LJ (1946) J Bacteriol 52:293–301PubMedPubMedCentralGoogle Scholar
  27. Zwieb C, Glotz C, Brimacombe R (1981) Nucleic Acids Res 9:3621–3640CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Jeffrey A. Knight
    • 1
  • Cathy J. Wedeen
    • 1
  • Kathryn A. Hughes
    • 1
  1. 1.Department of Biological SciencesMount Holyoke CollegeSouth HadleyUSA

Personalised recommendations