Current Genetics

, Volume 8, Issue 2, pp 93–97 | Cite as

Mapping of rRNA genes by integration of hybrid plasmids in Schizosaccharomyces pombe

  • Takashi Toda
  • Yukinobu Nakaseko
  • Osami Niwa
  • Mitsuhiro Yanagida


The major rRNA genes of the fission yeast Schizosaccharomyces pombe were mapped on chromosome III by plasmid integration. The integration vector YIp33 containing S. cerevisiae LEU2 gene was combined with the S. pombe rDNA. Since LEU2 complements S. pombe leu1 deficiency, it could be used as the genetic marker for integration. The 10.4 kb rDNA repeat contained ARS sequence, and therefore 2.4 kb and 0.7 kb subfragments not containing ARS were subcloned into YIp33 and transformed leu1 S. pombe cells to Leu+. Genetic analyses of the transformants indicated that the integrated rDNA resides in the long arm of the shortest chromosome III, tightly linked to ade5 (1.4 cM). This result is consistent with our previous finding that the DAPI-stained smallest chromosomes were associated with the nucleolus (Umesono et al. 1983).

Key words

Integration vector Nucleolar DNA rRNA gene mapping Schizosaccharomyces pombe 



autonomously replicating sequence




kilo base pairs


DNA segment containing ribosomal RNA genes


ribosomal RNA


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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Takashi Toda
    • 1
  • Yukinobu Nakaseko
    • 1
  • Osami Niwa
    • 1
  • Mitsuhiro Yanagida
    • 1
  1. 1.Department of Biophysics, Faculty of ScienceKyoto UniversitySakyo-ku, KyotoJapan

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