Molecular and General Genetics MGG

, Volume 117, Issue 4, pp 319–336 | Cite as

Morphogenesis of the tail fiber of bacteriophage T4

IV. Isolation and characterization of a component (the genes 36-37-38 directing component)
  • S. Imada
  • A. Tsugita


A component of T4 phage tail fiber was purified from the lysate of E. coli strain Bb infected with gene 35 defective mutant of T4D (amB252). The purified component which occupies a part of the distal half fiber is formed under the control of genes 36, 37 and 38. The purified component was characterized and compared with the genes 35-36-37-38 directed half fiber. Although the components resembled each other, differences were observed in length, stability and chemical compositions. The results of a further decomposition of this component and the correlating characters of the gene 35 and 36 directed products were discussed.


Directed Product Defective Mutant Tail Fiber Phage Tail Phage Tail Fiber 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Brenner, S., Champe, S. P., Streisinger, G., Barnett L.: On the interaction of adsorption cofactors with bacteriophage T2 and T4. Virology 17, 30–39 (1962).CrossRefGoogle Scholar
  2. Brenner, S., Streisinger, G., Horne, R. W., Champe, S. P., Barnet, L., Benzer, S., Rees, M. W.: Structural components of bacteriophage. J. molec. Biol. 1, 281–292 (1959).CrossRefGoogle Scholar
  3. Brückner, J.: Estimation of monosacharides by the orcinol-sulphuric acid reaction. Biochem. J. 60, 200–206 (1955).CrossRefGoogle Scholar
  4. Edgar, R. S., Leilausis, I.: Serological studies with mutants of phage T4D defective in genes determining tail fiber structure. Genetics 52, 1187–1200 (1965).PubMedPubMedCentralGoogle Scholar
  5. Edgar, R. S., Wood, W. B.: Morphogenesis of bacteriophage T4 in extracts of mutant-infected cells. Proc. nat. Acad. Sci. (Wash.) 55, 498–505 (1966).CrossRefGoogle Scholar
  6. Epstein, R. H., Bolle, A., Steinberg, C. M., Kellenberger, E., Boy de la Tour, E., Chevalley, R., Edgar, R. S., Susman, M., Denhardt, G. H., Lielausis, A.: Physiological studies of conditional lethal mutants of bacteriophage T4D. Cold. Spr. Harb. Symp. quant. Biol. 28, 375–394 (1963).CrossRefGoogle Scholar
  7. Goodwin, T. W., Morton, R. A.: The spectrophotometric determination of tyrosine and tryptophan in proteins. Biochem. J. 40, 628–631 (1946).CrossRefGoogle Scholar
  8. Hirs, C. H. W.: The oxidation of ribonuclease with performic acid. J. biol. Chem. 219, 611–621 (1956).PubMedGoogle Scholar
  9. Hosoda, J., Cone, R.: Analysis of T4 phage proteins, I. Conversion of precursor proteins into lower molecular weight peptides during normal capsid formation. Proc. nat. Acad. Sci. (Wash.) 66, 1275–1281 (1970).CrossRefGoogle Scholar
  10. Imada, S., Tsugita, A.: Morphogenesis of the tail fiber of bacteriophage T4. II. Isolation and characterization of a component (the Genes 35-36-37-38 directing half fiber). Molec. gen. Genet. 109, 338–355 (1970).CrossRefGoogle Scholar
  11. Inouye, M., Tsugita, A.: The amino acid sequence of T4 bacteriophage lysozyme. J. molec. Biol. 22, 193–1969 (1966).CrossRefGoogle Scholar
  12. Jones, T. H. D., Kennedy, E. P.: Characterization of the membrane protein component of the lactose transport system of Escherichia coli. J. biol. Chem. 244, 5981–5987 (1969).PubMedGoogle Scholar
  13. Kellenberger, E., Bolle, A., Boy de la Tour, E., Epstein, R. H., Franklin, N. C., Jerne, N. K., Reale-Scafati, A., Sechaud, J., Bendet, I., Goldstein, D., Lauffer, M. A.: Functions and properties related to the tail fibers of bacteriophage T4. Virology 26, 419–440 (1965).CrossRefGoogle Scholar
  14. King, J., Laemmli, U. K.: Polypeptides of the trail fibres of bacteriophage T4. J. molec. Biol. 62, 465–477 (1971).CrossRefGoogle Scholar
  15. King, J., Wood, W. B.: Assembly of bacteriophage T4 tail fibers: The sequence of gene product interaction. J. molec. Biol. 39, 583–601 (1969).CrossRefGoogle Scholar
  16. Lowry, O. H., Rosenbrough, A. L., Farr, N. J., Rendall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951).PubMedGoogle Scholar
  17. Schachmann, H. K.: Techniques for characterization of proteins: Ultracentrifugation, diffusion and viscometry. Meth. in Enzymol. 4, 32–103 (1959).CrossRefGoogle Scholar
  18. Shinomiya, T., Imada, S., Tsugita, A.: Isolation of the gene 35-product and the genes 36-37-38 product of the fiber component of T4 phage. Molec. gen. Genet. 111, 368–372 (1971).CrossRefGoogle Scholar
  19. Spies, J. R., Chambers, D. C.: Chemical determination of tryptophan in proteins. Analyt. Chem. 21, 1249–1256 (1949).CrossRefGoogle Scholar
  20. Takata, R., Tsugita, A.: Morphogenesis of the tail fiber of bacteriophage T4. I. Isolation and characterization of a component (the gene 34 product). J. molec. Biol. 54, 45–57 (1970).CrossRefGoogle Scholar
  21. Terzaghi, E., Tsugita, A.: In vitro assembly of T4 phage. Jap. J. Genet. 41, 484 (Abstr.) (1966).Google Scholar
  22. To, C. M.: Doctorate Thesis, Kansas State University. (1968).Google Scholar
  23. Ward, S., Wood, W. B.: Purification of an intermediate in phage T4 tail fiber assembly. Fed. Proc. 27, 592 (1968).Google Scholar
  24. Yamakawa, T., Ueta, N.: Gas-liquid chromatography of carbohydrates. Jap. J. exp. Med. 34, 37–51 (1964).PubMedGoogle Scholar
  25. Yanagida, M., Ahmad-Zadeh, C.: Determination of gene product positions in bacteriophage T4 by specific antibody association. J. molec. Biol. 51,411–420 (1970).CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • S. Imada
    • 1
  • A. Tsugita
    • 1
  1. 1.Laboratory of Molecular Genetics, Medical SchoolUniversity of OsakaOsakaJapan

Personalised recommendations