Summary
Ploidy patterns, analysed by flow cytometry (FCM) and image analysis (IA), were investigated at relapse in a group of six children with renal tumours [five Wilms' tumour (WT) and one bone metastasizing renal tumour of childhood (BMRTC)] and results compared with diagnostic profiles. IA detected one or more aneuploid populations in five of 12 tumours which were diploid on FCM. Patterns in three of six patients [two with unfavourable histology (UH) and one with favourable histology (FH)] were aneuploid at diagnosis and relapse, two patients (one FH, one BMRTC) developed aneuploid features at relapse and one patient with a tetraploid tumour was diploid at relapse. Histology patterns were similar at diagnosis and relapse in all patients. Three of six patients (two UH, one BMRTC) have died of disease. This report highlights (1) the superiority of IA over FCM in detecting aneuploid populations and (2) changes in ploidy status which have not previously been reported in these tumours. Overinterpretation of DNA status at relapse may prove misleading.
Similar content being viewed by others
References
Douglass E, Look AT, Webber B, Parham D, Wilimas JA, Green AA, Roberson PK (1986) Hyperdiploidy and chromosomal rearrangements define an anaplastic variant of Wilms' tumor. J Clin Oncol 4:975
Gonzalez-Crussi F, Baum ES (1983) Renal sarcomas of childhood: a clinicopathologic and ultrastructural study. Cancer 51:898
Gururangan S, Dorman A, Ball R, Curran B, Leader M, Breatnach F, O'Meara A (1992) DNA quantitation of Wilms' tumour (nephroblastoma) using flow cytometry and image analysis. J Clin Pathol 45:498
Kumar S, Marsden HB, Cowan RA, Barnes JM (1989) Prognostic relevance of DNA content in childhood renal tumours. Br J Cancer 59:291
Leeuwen EH van, Postma A, Oosterhuis JW, Meiring A, Cornelisse CJ, Koudstaal J (1987) An analysis of histology and DNA ploidy in primary Wilms' tumours and their metastases and a study of the morphological effects of therapy. Virchows Arch [A] 410:487
Pritchard J, Barnes JM, Gough D (1988) Preliminary results of the first UKCCSG Wilms' tumour study. Med Pediatr Oncol 16:413
Schmidt D, Wiedemann B, Keil W, Sprenger E, Harms D (1986) Flow cytometric analysis of nephroblastomas and related neoplasms. Cancer 58:2494
Taylor SR, Blatt J, Costantino JP, Roederer M, Murphy RF (1988) Flow cytometric DNA analysis of neuroblastoma and ganglioneuroma: a 10 year retrospective study. Cancer 62:749
Zuppan CW, Beckwith B, Weeks DA, Luckey DW, Pringle KC (1991) The effect of preoperative therapy on the histological features of Wilms' tumor: an analysis of cases from the third national Wilms' tumor study. Cancer 68:385
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
O'Meara, A., Gururangan, S., Ball, R. et al. Ploidy changes between diagnosis and relapse in childhood renal tumours. Urol. Res. 21, 345–347 (1993). https://doi.org/10.1007/BF00296834
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00296834