Controversy exists as to the morphological and morphometric changes seen in experimental diabetic neuropathy (EDN). Most previous studies have utilized immature animals, with controversy as to whether the observed changes are due to maturational delays induced by hyperglycemia, or to diabetes per se. This study utilizes mature 9-month-old Sprague-Dawley rats. Six control and six hyperglycemic rats were examined 24 weeks after streptozocin injection. No morphological abnormalities were seen in the sciatic nerve at the light microscopy level. Total fascicular area and myelinated fiber density showed no significant differences (ANOVA, P > 0.05). No significant differences [ANOVA, P > 0.05 and Kolmogorov-Smirnoff (K-S), P > 0.05] between control and diabetic groups were shown for fiber, axon, and myelin areas, fiber and axon diameters, and myelin thickness. Fiber index of circularity, axon index of circularity, and g ratio were not significantly different with ANOVA (P > 0.05), but the diabetic group showed significantly lower values (P < 0.001) with K-S testing. Regression analyses of axonal area and log(n) axonal area plotted against myelin thickness showed no significant differences between the control and diabetic animals. This study in mature rats confirms the relative lack of morphological and morphometric changes in EDN which have previously been reported in studies involving immature rats. It high-lights the difficulties in trying to extrapolate from EDN to human diabetic neuropathy where severe morphological and morphometric abnormalities may be present.
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Allpress S, Pollock M, Nukada H (1983) Morphometric analysis of peripheral nerve. In: Kidman AD, Tomkins JK, Morris CA, Cooper NA (eds) Molecular pathology of nerve and muscle. Human Press, Clifton, pp 131–161
Bestetti G, Rossi GL, Zemp C (1981) Changes in peripheral nerves of rats four months after induction of streptozocin diabetes. A qualitative and quantitative study. Acta Neuropathol (Berl) 54: 129–134
Bestetti G, Zemp C, Probst D, Rossi GL (1981) Neuropathy and myopathy in the diaphragm of rats after 12 months of streptozocin-induced diabetes mellitus. A light, electron microscopic and morphometric study. Acta Neuropathol (Berl) 55: 11–20
Brown MJ, Summer AJ, Greene DA, Diamond SM, Asbury AK (1980) Distal neuropathy in experimental diabetes mellitus. Ann Neurol 8: 168–178
Dockery P, Sharma AK (1990) Ultrastructural abnormalities of myelinated fibres in the tibial nerve of streptozotocin-diabetic rats. J Neurol Sci 98: 327–345
Dyck PJ, Lambert EH, Nichols PC (1971) Quantitative measurement of sensation related to compound action potential and number and sizes of myelinated and unmyelinated fibers of sural nerves in health, Friedreich's ataxia, hereditary sensory neuropathy, and tabes dorsalis. In: Cobb WA (ed) Handbook of electroencephalography and clinical neurophysiology. Elsevier, Amsterdam, pp 83–118
Dyck PJ, Karnes J, Lais A, Lofgren EP, Stevens JC (1984) Pathologic alterations of the peripheral nervous system of humans. In: Dyck PJ, Thomas PK, Lambert EH, Bunge R (eds) Peripheral neuropathy, 2nd edn. Saunders, Philadelphia, pp 760–870
Jakobsen J (1976) Axonal dwindling in early experimental diabetes. I. A study of cross sectioned nerves. Diabetologia 12: 539–546
Jakobsen J (1978) Peripheral nerves in early experimental diabetes. Expansion of the endoneurial space as a cause of increased water content. Diabetologia 14: 113–119
Mayhew TM (1990) Efficient and unbiased sampling of nerve fibers for estimating fiber number and size. In: Conn PM (ed) Methods in neuroscience, vol 3. Quantitative and qualitative microscopy. Academic Press, New York, pp 172–187
Nukada H, Powell HC, Myers RR (1992) Perineurial window: demyelination in nonherniated endoneurium with reduced nerve blood flow. J Neuropathol Exp Neurol 51: 523–530
Pollock M, Dyck PJ (1976) Peripheral nerve morphometry in myotonic dystrophy. Arch Neurol 33: 33–39
Powell HC, Myers RR (1985) Axonopathy and microangiopathy in chronic alloxan diabetes. Acta Neuropathol (Berl) 68: 295–305
Sharma AK, Thomas PK (1974) Peripheral nerve structure and function in experimental diabetes. J Neurol Sci 23: 1–15
Sharma AK, Thomas PK (1987) Animal models: pathology and pathophysiology. In: Dyck PJ, Thomas PK, Asbury AK, Winegrad AI, Porte D Jr (eds) Diabetic neuropathy. Saunders, Philadelphia, pp 237–252
Sharma AK, Thomas PK, De Molina AF (1977) Peripheral nerve fiber size in experimental diabetes. Diabetes 26: 689–692
Sharma AK, Bajada S, Thomas PK (1980) Age changes in the tibial and plantar nerves of the rat. J Anat 130: 417–428
Sharma AK, Bajada S, Thomas PK (1981) Influence of streptozotocin-induced diabetes on myelinated nerve fibre maturation and on body growth in the rat. Acta Neuropathol (Berl) 53: 257–265
Sharma AK, Duguid IGM, Blanchard DS, Thomas PK (1985) The effect of insulin treatment on myelinated nerve fiber maturation and integrity and on body growth in streptozotocin-diabetic rats. J Neurol Sci 67: 285–297
Siegel S, Castellan NJ Jr (1988) Nonparametric statistics for the behavioral sciences, 2nd edn. McGraw-Hill, New York, pp 145–147
Sima AAF, Zhang W, Tze WJ, Tai J, Nathaniel V (1988) Diabetic neuropathy in STZ-induced diabetic rat and effect of allogeneic islet cell transplantation: morphometric analysis. Diabetes 37: 1129–1136
Sugimura K, Windebank AJ, Natarajan V, Lambert EH, Schmid HHO, Dyck PJ (1980) Interstitial hyperosmolarity may cause axis cylinder shrinkage in streptozotocin diabetic nerve. J Neuropathol Exp Neurol 39: 710–721
Thomas PK (1990) The pathogenesis of diabetic neuropathy: current problems and prospects. In: Ward J, Goto Y (eds) Diabetic neuropathy. Wiley, Chichester, pp 3–14
Thomas PK, Eliasson SG (1984) Diabetic neuropathy. In: Dyck PJ, Thomas PK, Lambert EH, Bunge R (eds) Peripheral neuropathy, 2nd edn. Saunders, Philadelphia, pp 1773–1810
Thomas PK, Fraher JP, O'Leary D, Moran MA, Cole M, King RHM (1990) Relative growth and maturation of axon size and myelin thickness in the tibial nerve of the rat. 2. Effect of streptozotocin-induced diabetes. Acta Neuropathol 79: 375–386
Torch S, Stoebner P, Usson Y, Druet D'Aubigny G, Saxod R (1989) There is no simple adequate sampling scheme for estimating the myelinated fiber size distribution in human peripheral nerve: a statistical ultrastructural study. J Neurosci Methods 27: 149–164
Zemp C, Bestetti G, Rossi GL (1981) Morphological and morphometric study of peripheral nerves from rats with streptozocin-induced diabetes mellitus. Acta Neuropathol (Berl) 53: 99–106
Supported by the Health Research Council of New Zealand (HRC 91/134), Otago Medical Research Foundation, and the Henderson Trust. Dr. Wright was the recipient of a Frances G. Cotter Scholarship and a Health Research Council of New Zealand training fellowship.
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Wright, A., Nukada, H. Sciatic nerve morphology and morphometry in mature rats with streptozocin-induced diabetes. Acta Neuropathol 88, 571–578 (1994). https://doi.org/10.1007/BF00296495
- Diabetic neuropathy
- Sciatic nerve