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Acute response of the fetal telencephalon to short-term maternal exposure to ethanol in the rat

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Summary

Pregnant rats were exposed to either ethanol (total dose 18 g/kg) on gestational days 14 and 15 or whole-body ionizing radiation (0.5 Gy) on gestational day 15. On gestational day 16, 24 h following the last dose of ethanol or exposure to ionizing radiation, the developing cerebral cortex of the fetus was examined histologically. Ionizing radiation caused extensive cell death within the fetal cerebral cortex whereas ethanol caused more subtle morphological changes such as cortical thinning and petechial intraventricular hemorrhages. These findings suggest that ethanol, unlike ionizing radiation, acts by some mechanism other than cell death to cause cortical thinning and cortical malformations. The pathogenesis of ethanol-induced cortical dysgenesis may include fetal hypoxia and inhibition of neuroblast proliferation within the developing cerebral cortex.

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References

  1. 1.

    Bannigan J, Cottell D (1984) Ethanol teratogenicity in mice: an electron microscopic study. Teratology 30: 281–290

  2. 2.

    Baroana E, Pirola RC, Lieber CS (1974) Small intestinal damage and changes in cell population produced by ethanol ingestion in the rat. Gastroenterology 66: 226–234

  3. 3.

    Berry M (1974) Development of the cerebral neocortex of the rat. In: Gottlieb G (ed) Aspects of neurogenesis. Academic Press, New York, pp 7–67

  4. 4.

    Berry M, Rogers AW (1965) The migration of neuroblasts in the developing cerebral cortex. J Anat 99: 691–709

  5. 5.

    Brown NS, Goulding EH, Fabro S (1979) Ethanol embryotoxicity: direct effects on mammalian embryos in vitro. Science 206: 573–575

  6. 6.

    Clarren SK, Bowden DM (1984) Measures of alcohol damage in utero in the pigtailed macaque (Macaca nemestrina). Ciba Found Symp 105: 157–172

  7. 7.

    Clarren SK, Alvord EC, Sumi SM, Streissguth AP, Smith DW (1978) Brain malformations related to prenatal exposure to ethanol. J Pediatr 92: 64–67

  8. 8.

    Dreosti IE, Ballard J, Belling GB, Record IR, Manuel SJ, Hetzel BS (1984) A single dose of ethanol suppresses rat embryo development in vivo. Alcohol Clin Exp Res 8: 326–329

  9. 9.

    Friede RL (1989) Developmental neuropathology, 2nd edn. Springer, New York, pp 44–58

  10. 10.

    Gibbons JD (1976) Nonparametric methods for quantitative analysis. Holt, Rinehart and Winston, New York, pp 157–204

  11. 11.

    Guerri C, Sanchis R (1985) Acetaldehyde and alcohol levels in pregnant rats and their fetuses. Alcohol 2: 267–270

  12. 12.

    Hammer RP, Scheibel AB, (1981) Morphologic evidence for a delay of neuronal maturation in fetal alcohol exposure. Exp Neurol 74: 587–596

  13. 13.

    Hicks SP (1954) The effects of ionizing radiation, certain hormones and radiomimetic drugs on the developing nervous system. J Cell Comp Physiol [Suppl] 43: 151–178

  14. 14.

    Hicks SP, D'Amato CJ (1963) Low dose radiation of the developing brain. Science 141: 903–905

  15. 15.

    Hicks SP, D'Amato CJ, Lowe MJ (1959) The development of the mammalian nervous system. J Comp Neurol 113: 435–470

  16. 16.

    Hicks SP, D'Amato CJ, Falk JL (1962) Some effects of radiation on structural and behavioral development. Int J Neurol 3: 535–548

  17. 17.

    Hildebrand R (1980) Nuclear volume and cellular metabolism. Adv Anat Embryol Cell Biol 60: 1–54

  18. 18.

    Iosub S, Fuchs M, Bingol N, Stone RK, Gromisch DS (1981) Long-term follow-up of three siblings with fetal alcohol syndrome. Alcohol Clin Exp Res 5: 523–527

  19. 19.

    Jacobi JM, Lloyd HM, Meares JD (1982) Nuclear diameter in the anterior pituitary gland of the rat: effects of estrogen, bromocriptine, and haloperidol. J Histochem Cytochem 30: 677–681

  20. 20.

    Jones KL, Smith DW, Ulleland CN, Streissguth AP (1973) Pattern of malformation in offspring of chronic alcoholic mothers. Lancet I: 1267–1271

  21. 21.

    Jones PJH, Leichter J, Lee M (1981) Placental blood flow in rats fed alcohol before and during gestation. Life Sci 29: 1153–1159

  22. 22.

    Karnovsky MJ (1965) A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J Cell Biol 27: 137A-138A

  23. 23.

    Kesäniemi YA, Sippel HW (1975) Placental and foetal metabolism of acetaldehyde in rat. I. Contents of ethanol and acetaldehyde in placenta and foetus of the pregnant rat during ethanol oxidation. Acta Pharmacol Toxicol 37: 43–48

  24. 24.

    Kotkoskie LA, Norton S (1988) Prenatal brain malformations following acute ethanol exposure in the rat. Alcohol Clin Exp Res 12: 831–836

  25. 25.

    Miller MW (1987) Effect of prenatal exposure to alcohol on the distribution and time of origin of corticospinal neurons in the rat. J Comp Neurol 257: 272–282

  26. 26.

    Mukherjee AB, Hodgen GD (1982) Maternal ethanol exposure induces transient impairment of umbilical circulation and fetal hypoxia in monkeys. Science 218: 700–702

  27. 27.

    Norton S, Kimler BF (1988) Comparison of functional and morphological deficits in the rat after gestational exposure to ionizing radiation. Neurotoxicol Teratol 10: 363–371

  28. 28.

    Norton S, Terranova P, Na JY, Sancho-Tello M (1988) Early motor development and cerebral cortical morphology in rats exposed perinatally to alcohol. Alcohol Clin Exp Res 12: 130–137

  29. 29.

    Peiffer J, Majewski F, Fishbach H, Bierich JR, Volk B (1979) Alcohol embryo- and fetopathy: neuropathology of 3 children and 3 fetuses. J Neurol Sci 41: 125–137

  30. 30.

    Pennington SN, Kalmus GW (1987) Brain growth during ethanol-induced hypoplasia. Drug Alcohol Depend 20: 279–286

  31. 31.

    Pennington SN, Boyd JW, Kalmus GW, Wilson RW (1983) The molecular mechanism of fetal alcohol syndrome (FAS) I. Ethanol-induced growth suppression. Neurobehav Toxicol Teratol 5: 259–262

  32. 32.

    Pratt OE, Doshi R (1984) Range of alcohol-induced damage in the developing central nervous system. Ciba Found Symp 105: 142–156

  33. 33.

    Siegel S (1956) Nonparametric statistics for the behavioral sciences. McGraw Hill, New York, pp 127–136

  34. 34.

    Streissguth AP, Herman CS, Smith DW 1978) Intelligence, behavior, and dysmorphogenesis in the fetal alcohol syndrome: a report on 20 patients. J Pediatr 92: 363–367

  35. 35.

    Streissguth AP, Clarren SK, Jones KL (1985) Natural history of the fetal alcohol syndrome: a 10-year follow-up of 11 patients. Lancet II: 85–91

  36. 36.

    Volk B, Malctz J, Tiedemann M, Mall G, Klein C, Berlet HH (1981) Impaired maturation of Purkinje cells in the fetal alcohol syndrome of the rat. Acta Neuropathol (Berl) 54: 19–29

  37. 37.

    Volpe JJ (1977) Observing the infant in the early hours after asphyxia. In: Gluck L (ed) Intrauterine asphyxia and the developing brain. Year Book Medical Publishers, Chicago, pp 262–283

  38. 38.

    Yamada M, Puck TT (1961) Action of radiation on mammalian cells. IV. Reversible mitotic lag in the S3 HELA cell produced by low doses of X-rays. Proc Natl Acad Sci USA 47: 1181–1191

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Author information

Correspondence to S. Norton.

Additional information

Supported in part by NS16694 and ES07079. LAK was supported by a Proctor and Gamble Toxicology Fellowship

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Kotkoskie, L.A., Norton, S. Acute response of the fetal telencephalon to short-term maternal exposure to ethanol in the rat. Acta Neuropathol 79, 513–519 (1990). https://doi.org/10.1007/BF00296111

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Key words

  • Fetal alcohol syndrome
  • Ethanol
  • Ionizing radiation
  • Cerebral cortex