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Inhibition of cell division and induction of chromosome aberrations in cultured Ehrlich ascites tumor cells by deoxyguanosine

Summary

Exposure of exponentially growing cultures of Ehrlich ascites tumor cells to 1 or 2×10-3 M deoxyguanosine resulted in an inhibition of DNA synthesis and cell multiplication. Continued increase in the RNA and protein content of these cultures suggests a state of “unbalanced growth”. Deoxyguanosine-inhibition is prevented by the presence of deoxycytidine (1×10-4–2×10-3 M).

Treatment with deoxyguanosine (2×10-3 M) for about one generation-time (18 hrs) and removal of deoxyguanosine thereafter resulted in chromosome aberrations (breaks and exchange figures) in 30–50% of those mitotic cells which were harvested 5 to 12 hrs after treatment. Chromosome defects were strongly reduced after incubation of cell-cultures in the presence of deoxyguanosine (2×10-3 M) together with deoxycytidine (5×10-4 M).

The biochemical mechanisms by which deoxyguanosine and other inhibitors of DNA synthesis might produce chromosome damaging effects, are discussed.

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Supported by grants from Deutsche Forschungsgemeinschaft (Scha 176/1 and Scha 176/2).

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Schachtschabel, D.O., Cunze, P. Inhibition of cell division and induction of chromosome aberrations in cultured Ehrlich ascites tumor cells by deoxyguanosine. Hum Genet 10, 127–137 (1970). https://doi.org/10.1007/BF00295511

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Keywords

  • Tumor Cell
  • Internal Medicine
  • Protein Content
  • Cell Division
  • Metabolic Disease