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Production of β-glucosidase by Aspergillus terreus


The production of β-glucosidase by Aspergillus terreus was investigated in liquid shake cultures. Enzyme production was maximum on the 7th day of growth (2.18 U/ml) with the initial pH of the medium in the range of 4.0–5.5. Cellulose (Sigmacell Type 100) at 1.0% (wt/vol) gave maximum β-glucosidase activity among the various soluble and insoluble carbon sources tested. Potassium nitrate was a suitable nitrogen source for enzyme production. Triton X-100 at 0.15% (vol/vol) increased the enzyme levels of A. terreus. The test fungal strain showed an ability to ferment glucose to ethanol.

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Literature Cited

  1. 1.

    Christakopoulos P, Macris BJ, Kekos D (1989) Direct fermentation of cellulose to ethanol by Fusarium oxysporum. Enzyme Microb Technol 11:236–239

  2. 2.

    Coughlan MP (1990) Cellulose degradation by fungi. In: Fogarty WM, Kelly CT (eds) Microbial enzymes and biotechnology. New York: Elsevier Science Publishing Co, Inc., pp 1–36

  3. 3.

    Dekker RFH (1981) Induction, localization and characterization of β-d-glucosidases produced by a species of Monilia. J Gen Microbiol 127:177–184

  4. 4.

    Desai JD, Desai AJ, Patel NP (1982) Production of cellulases and β-glucosidase by shake culture of Scytalidium lignicola. J Ferment Technol 60:117–124

  5. 5.

    Ghose TK (ed) (1977) Bioconversion of cellulosic substances into energy, chemical and microbial proteins: IIT symp. New Delhi, India: Thompson Press

  6. 6.

    Gong CS, Maun CM, Tsao GT (1981) Direct fermentation of cellulose to ethanol by a cellulolytic filamentous fungus, Monilia sp. Biotechnol Lett 3:77–82

  7. 7.

    Hagerdahl BGR, Ferchak JD, Pye BK (1978) Cellulolytic enzyme system of Thermoactinomyces sp. grown on microcrystalline cellulose. Appl Environ Microbiol 36:606–612

  8. 8.

    Hamilton LA, Wase DAJ (1991) Some comparisons of cellulases from two different strains of Aspergillus fumigatus. Process Biochem 26:287–292

  9. 9.

    Kagi JHR, Vallee BL (1960) The role of zinc in alcohol dehydrogenase. J Biol Chem 235:3188–3192

  10. 10.

    Lloyd JB, Whelan WJ (1969) An improved method for enzymic determination of glucose in the presence of maltose. Anal Biochem 30:467–470

  11. 11.

    Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurements with Folin phenol reagent. J Biol Chem 193:265–275

  12. 12.

    Mandels M, Weber J (1969) The production of cellulases. Adv Chem Ser 95:391–413

  13. 13.

    Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal Chem 31:426–428

  14. 14.

    Nisizawa T, Suzuki H, Nisizawa K (1972) Catabolite repression of cellulase formation in Trichoderma viride. J Biochem 71:999–1002

  15. 15.

    Okeke BC, Obi SKC (1993) Production of cellulolytic and xylanolytic enzymes by an Arthrographis sp. World J Microbiol Biotechnol 9:345–349

  16. 16.

    Reese ET, Maguire A (1971) Increase in cellulase yields by addition of surfactants to cellobiose cultures of Trichoderma viride. Dev Ind Microbiol 12:212–224

  17. 17.

    Srivatsava SK, Gopalkrishnan KS, Ramachandran KB (1987) The production of β-glucosidase in shake flasks by Aspergillus wentii. J Ferment Technol 65:95–99

  18. 18.

    Trivedi LS, Rao KK (1979) Production of cellulolytic enzymes by Aspergillus fumigatus. Indian J Exp Biol 71:671–674

  19. 19.

    Trivedi LS, Rao KK (1981) Production of cellulolytic enzymes by Fusarium sp. Biotechnol Lett 3:281–284

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Pushalkar, S., Rao, K.K. & Menon, K. Production of β-glucosidase by Aspergillus terreus . Current Microbiology 30, 255–258 (1995).

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  • Cellulose
  • Aspergillus
  • Nitrogen Source
  • Enzyme Production
  • Fungal Strain