The production of β-glucosidase by Aspergillus terreus was investigated in liquid shake cultures. Enzyme production was maximum on the 7th day of growth (2.18 U/ml) with the initial pH of the medium in the range of 4.0–5.5. Cellulose (Sigmacell Type 100) at 1.0% (wt/vol) gave maximum β-glucosidase activity among the various soluble and insoluble carbon sources tested. Potassium nitrate was a suitable nitrogen source for enzyme production. Triton X-100 at 0.15% (vol/vol) increased the enzyme levels of A. terreus. The test fungal strain showed an ability to ferment glucose to ethanol.
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Pushalkar, S., Rao, K.K. & Menon, K. Production of β-glucosidase by Aspergillus terreus . Current Microbiology 30, 255–258 (1995). https://doi.org/10.1007/BF00295497
- Nitrogen Source
- Enzyme Production
- Fungal Strain