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Studies on frequency of Y chromatin in human sperm

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The preparation and evaluation of human ejaculate smears on cover glasses enables double-sided examination of the individual sperm heads in the fluorescence microscope. By this method of observation the frequency of Y chromatin in the ejaculates of 19 probands increased significantly to between 1.7 and 6.3%. However, the frequency of 50% Y chromatin-positive sperms theoretically to be expected is not found by applying this technique.

Furthermore, sexual abstention of at least 14 days leads to a statistically significant decrease of the frequency of Y chromatin. These observations could explain why the data on Y chromatin frequency reported in the literature appear so inconsistent.

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Supported by the Deutsche Forschungsgemeinschaft (AZ Schw. 154/4).

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Schwinger, E., Ites, J. & Korte, B. Studies on frequency of Y chromatin in human sperm. Hum Genet 34, 265–270 (1976).

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  • Internal Medicine
  • Fluorescence Microscope
  • Metabolic Disease
  • Cover Glass
  • Human Sperm