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Studies on frequency of Y chromatin in human sperm

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Summary

The preparation and evaluation of human ejaculate smears on cover glasses enables double-sided examination of the individual sperm heads in the fluorescence microscope. By this method of observation the frequency of Y chromatin in the ejaculates of 19 probands increased significantly to between 1.7 and 6.3%. However, the frequency of 50% Y chromatin-positive sperms theoretically to be expected is not found by applying this technique.

Furthermore, sexual abstention of at least 14 days leads to a statistically significant decrease of the frequency of Y chromatin. These observations could explain why the data on Y chromatin frequency reported in the literature appear so inconsistent.

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References

  1. Barlow, P., Vosa, C. G.: The Y-chromosome in human spermatozoa. Nature (Lond.) 226, 961–962 (1970)

  2. Buettinghaus, K., Mauss, J.: Occurrence of Y and nY spermatozoa in subfertile men. Fortschr. Med. 93, 320–323 (1975)

  3. Caspersson, T., Zech, L., Johansson, C.: Differential binding of alkylating fluorochromes in human chromosomes. Exp. Cell Res. 60, 315–319 (1970)

  4. Diasio, R. B., Glass, R. H.: Effect of pH on the migration of X and Y sperm. Fertil. and Steril. 22, 303–305 (1971)

  5. Doepfmer, R., Schuermann, H.: Handbuch der Haut- und Geschlechtskrankheiten. Ergänzungswerk, Vol. 6/3 (ed. J. Jadassohn). Berlin-Heidelberg-New York: Springer 1960

  6. Ericsson, R. J., Langevin, C. N., Nishino, M.: Isolation of fractions rich in human Y sperm. Nature (Lond.) 246, 421–424 (1973)

  7. Kaiser, R., Citoler, P., Broer, K. H.: Relative increase in Y chromatin-bearing spermatozoa after in vivo penetration into human cervical mucus. IRCS 2, 1100 (1974)

  8. Leslie, W., Quinlivan, G., Sullivan, H.: The ratios and separations of X and Y spermatozoa in human semen. Fertil. and Steril. 25, 315–318 (1974)

  9. Moscetti, G., Aghemo, G., Ronzoni, P.: Observations in human spermatozoa by fluorescent “acranil” staining. A preliminary report. Humangenetik 17, 75–77 (1972)

  10. Pawlowitzki, I. H., Pearson, P. L.: Chromosomal aneuploidy in human spermatozoa. Humangenetik 16, 119–121 (1972)

  11. Pearson, P. L., Bobrow, M.: Fluorescent staining of the Y chromosome in meiotic stages of the human male. J. Reprod. Fertil. 22, 177–179 (1970a)

  12. Pearson, P. L., Bobrow, M., Vosa, C. G.: Technique for identifying Y chromosomes in human interphase nuclei. Nature (Lond.) 226, 78–80 (1970b)

  13. Rohde, W., Porstmann, T., Prehn, S., Dörner, G.: Gravitational pattern of the Y-bearing human spermatozoa in density gradient centrifugation. J. Reprod. Fertil. 42, 587–591 (1975)

  14. Schwinger, E.: Bedeutung und gerichtsmedizinische Anwendung der DNS-Fluorochromierung von Chromosomen und Zellkernen. Arbeitsmethoden der medizinischen und naturwissenschaftlichen Kriminalistik, Vol. 13. Lübeck: Schmidt-Römhild 1975

  15. Steeno, O., Achinwelja, A., Steeno, J.: Separation of X- and Y-bearing human spermatozoa with the sephadex gelfiltration method. Andrologia 7, 95–97 (1975)

  16. Sumner, A. T., Robinson, J. A., Evans, H. J.: Distinguishing between X, Y and YY-bearing human spermatozoa by fluorescence and DNA Content. Nature New Biol. (Lond.) 229, 231–233 (1971)

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Supported by the Deutsche Forschungsgemeinschaft (AZ Schw. 154/4).

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Schwinger, E., Ites, J. & Korte, B. Studies on frequency of Y chromatin in human sperm. Hum Genet 34, 265–270 (1976). https://doi.org/10.1007/BF00295289

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Keywords

  • Internal Medicine
  • Fluorescence Microscope
  • Metabolic Disease
  • Cover Glass
  • Human Sperm