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Difference between diploid and aneuploid Chinese hamster cells in replication at mid-S-phase

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Following partial synchronization of the heteroploid Chinese hamster cell line V-79 and of normal diploid lung fibroblasts of the Chinese hamster in culture, their DNA replication during S-phase was compared by means of a BrdU-incorporation/thymidine pulse technique and Hoechst-Giemsa differential staining of metaphase chromosomes. This comparison indirectly shows the S-phase of the heteroploid cells of V-79 to be 2 h shorter than the diploid cell S-phase. When the thymidine pulse is applied to diploid lung fibroblasts at mid-S-phase, differential staining colours metaphase chromosomes a pale blue. Performing the corresponding experiment with V-79 cells, neither a pale blue nor dark red staining is obtained, but rather an intermediate shade, showing prominently dark staining regions in parts. The pause in DNA synthesis observed at mid-S-phase of the diploid Chinese hamster lung fibroblasts seems to be omitted at mid-S-phase of the V-79 cells.

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  1. Campbell, C.E., Worton, R.G.: Chromosome replication patterns in an established cell line (CHO). Cytogenet. Cell Genet. 19, 303–319 (1977)

  2. Cohn, N.S.: DNA synthetic rates among cells of populations in vitro. Experientia (Basel) 24, 822–824 (1968)

  3. Crossen, P.E., Pathak, S., Arrighi, F.E.: A high resolution study of the DNA replication patterns of Chinese hamster chromosomes using sister chromatid differential staining technique. Chromosoma (Berl.) 52, 339–347 (1975)

  4. Epplen, J.T., Siebers, J.-W., Vogel, W.: DNA replication patterns of human chromosomes from fibroblasts and amniotic fluid cells revealed by a Giemsa staining technique. Cytogenet. Cell Genet. 15, 177–185 (1975)

  5. Fernández-Gómez, M.E., Stockert, J.C., Gonzáles-Fernández, A., López-Sáez, J.F.: Delays in prophase induced adenosine 2-deoxyriboside and their relation with DNA synthesis. Chromosoma (Berl.) 29, 1–11 (1970)

  6. Hamilton, A.J.: Cell population kinetics: A modified interpretation of the graph of labeled mitoses. Science 164, 952–954 (1969)

  7. Howard, A., Dewey, D.L.: Non uniformity of labelling rate during DNA synthesis. Exp. Cell Res. 24, 623–624 (1961)

  8. Kapp, L.N., Painter, R.B.: Multiple thymidine incorporation peaks in the S-phase of synchronous human diploid fibroblasts. Exp. Cell Res. 107, 429–431 (1977)

  9. Klevecz, R.R.: Temporal coordination of DNA replication with enzyme synthesis in diploid and heteroploid cells. Science 166, 1536–1538 (1969)

  10. Klevecz, R.R.: An automated system for cell cycle analysis. Anal. Biochem. 49, 407–415 (1972)

  11. Klevecz, R.R., Kapp, L.N.: Intermittent DNA synthesis and periodic expression of enzyme activity in the cell cycle of WI-38. J. Cell Biol. 58, 564–573 (1973)

  12. Latt, S.A.: Microfluorometric detection of deoxyribonucleic acid replication in human metaphase chromosomes. Proc. nat. Acad. Sci. (Wash.) 70, 3395–3399 (1973)

  13. Latt, S.A.: Microfluorometric analysis of deoxyribonucleic acid replication kinetics and sister chromatid exchanges in human chromosomes. J. Histochem. Cytochem. 22, 478–491 (1974)

  14. Painter, R.B., Schaefer, A.W.: Variation in the rate of DNA chain growth through the S phase in HeLa cells. J. molec. Biol. 58, 289–295 (1971)

  15. Remington, J.A., Flickinger, R.A.: The time of DNA replication in the cell cycle in relation to DNA synthesis in Frog embryos. J. Cell Physiol. 77, 411–422 (1971)

  16. Remington, J.A., Klevecz, R.R.: Families of replicating units in cultured hamster fibroblasts. Exp. Cell Res. 76, 410–418 (1973)

  17. Schaer, J.C., Ramseier, L., Schindler, R.: Studies on the division cycle of mammalian cells. IV. Incorporation of labeled precursors into DNA of synchronously dividing cells in culture. Exp. Cell Res. 65, 17–22 (1971)

  18. Schempp, W., Vogel, W.: Decrease of DNA synthesis in amniotic fluid cells during the middle part of S phase revealed by differential chromosome staining after incorporation of BrdU. Chromosoma (Berl.) 67, 193–199 (1978)

  19. Stubblefield, E.: Analysis of the replication pattern of Chinese hamster chromosomes using 5-bromodeoxyuridine suppression of 33258 Hoechst fluorescence. Chromosoma (Berl.) 53, 209–221 (1975)

  20. Stubblefield, E., Klevecz, R.: Synchronization of Chinese hamster cells by reversal of colcemid inhibition. Exp. Cell Res. 40, 660–664 (1965)

  21. Stubblefield, E., Gay, M.: Quantitative tritium autoradiography of mammalian chromosomes. II. The kinetics of DNA synthesis in individual chromosomes of Chinese hamster fibroblasts. Chromosoma (Berl.) 31, 79–90 (1970)

  22. Taylor, J.H.: Asynchronous duplication of chromosomes in cultured cells of Chinese hamster. J. biophys. biochem. Cytol. 7, 455–464 (1960)

  23. Terasima, T., Tolmach, L.J.: Growth and nucleic acid synthesis in synchronously dividing populations of HeLa cells. Exp. Cell Res. 30, 344–362 (1963)

  24. Vogel, W., Schempp, W., Sigwarth, I.: Comparison of thymidine, fluorodeoxyuridine, hydroxyurea and methotrexate blocking at the G1-/S-phase transition of cell cycle studied by replication patterns. Hum. Genet. 45, 193–198 (1978)

  25. Wolf, U.: Cell cultures from tissue explants: Culture and preparation of cells from amniotic fluid. In: Methods in human cytogenetics (H.G. Schwarzacher and U. Wolf, eds.). Berlin-Heidelberg-New York: Springer 1974

  26. Zeuthen, E.: Thymine starvation by inhibition of uptake and synthesis of thymine compounds in Tetrahymena. Exp. Cell Res. 50, 37–46 (1968)

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Correspondence to W. Vogel.

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Schempp, W., Vogel, W. Difference between diploid and aneuploid Chinese hamster cells in replication at mid-S-phase. Chromosoma 73, 109–115 (1979). https://doi.org/10.1007/BF00294850

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  • Thymidine
  • Lung Fibroblast
  • Metaphase Chromosome
  • Diploid Cell
  • Chinese Hamster Cell