Advertisement

Springer Nature is making Coronavirus research free. View research | View latest news | Sign up for updates

The intra-nucleolar localization of amplified rDNA in Xenopus laevis oocytes

  • 38 Accesses

  • 12 Citations

Abstract

A fluorescent Feulgen-stain was adapted in order to demonstrate DNA-containing structures inside the amplified nucleoli of Xenopus laevis. At all stages of oogenesis this method reveals granules or complex structures of DNA in each nucleolus. The micronucleoli which do not stain with this method and which do not reveal an internal structure in low molarity saline, unlike real nucleoli are considered as nucleolus-like bodies. The DNA-containing structures in the nucleoli can be composed of one or several granules, or they can be arranged in a linear, reticulated or circular form, independant of any correlation with the stage of oogenesis.

This is a preview of subscription content, log in to check access.

References

  1. Bird, A.P., Birnstiel, M.L.: A timing study of DNA amplification in Xenopus laevis oocytes. Chromosoma (Berl.) 35, 300–309 (1971)

  2. Brachet, J.: La localisation de l'acide thymonucléique pendant l'oogénèse et la maturation chez les amphibiens. Arch. Biol. 51, 151–165 (1940)

  3. Brown, D.D., Littna, E.: Variations in the synthesis of stable RNAs during oogenesis and development of Xenopus laevis. J. molec. Biol. 8, 688–695 (1964)

  4. Callan, H.G., Lloyd, L.: Lampbrush chromosomes of crested newts Trituras cristatus (Laurenti). Phil. Trans. roy. Soc. B 243, 135–219 (1960)

  5. Crissman, H.A., Tobey, R.A.: Cell-cycle analysis in 20 minutes. Science 184, 1297–1298 (1974)

  6. Davidson, E.H., Allfrey, V.G., Mirsky, A.E.: On the RNA synthesized during the lampbrush phase of amphibian oogenesis. Proc. nat. Acad. Sci. (Wash.) 52, 501–508 (1964)

  7. Dumont, J.N.: Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory-maintained animals. J. Morph. 136, 153–180 (1972)

  8. Ebstein, B.S.: The distribution of DNA within the nucleoli of the amphibian oocyte as demonstrated by tritiated actinomycin D radioautography. J. Cell Sci. 5, 27–44 (1969)

  9. Evans, D., Birnstiel, M.L.: Localization of amplified ribosomal DNA in the oocyte of Xenopus laevis. Biochim. biophys. Acta (Amst.) 166, 274–276 (1968)

  10. Fischberg, M., Wallace, H.: A mutation which reduces nucleolar number in Xenopus laevis. In: The Cell Nucleus (J.S. Mitchell, ed.), p. 30–34. London: Butterworth 1960

  11. Guyenot, E., Danon, M.: Chromosomes et ovocytes des Batraciens. Rev. Suisse Zool. 60, 1–129 (1953)

  12. Hay, E.D., Gurdon, J.B.: Fine structure of the nucleolus in normal and mutant Xenopus embryos. J. Cell Sci. 2, 151–162 (1967)

  13. Kalt, M.R., Gall, J.G.: Observations on early germ cell development and premeiotic ribosomal DNA amplification in Xenopus laevis. J. Cell Biol. 62, 460–472 (1974)

  14. Macgregor, H.C.: Nucleolar DNA in oocytes of Xenopus laevis. J. Cell Sci. 3, 437–444 (1968)

  15. Mairy, M., Denis, H.: Recherches biochimiques sur l'oogénèse. I. Synthèse et accumulation du RNA pendant l'oogénèse du crapaud sud-africain Xenopus laevis. Develop. Biol. 24, 143–165 (1971)

  16. Miller, L., Gonzales, F.: The relationship of ribosomal RNA synthesis to the formation of segregated nucleoli and nucleolus-like bodies. J. Cell Biol. 71, 939–949 (1976)

  17. Miller, O.L.: Structure and composition of peripheral nucleoli of salamander oocytes. Nat. Cancer Inst. Monogr. 23, 53–66 (1966)

  18. Painter, T.S., Taylor, A.N.: Nucleic acid storage in the toad's eggs. Proc. nat. Acad. Sci. (Wash.) 28, 311–317 (1942)

  19. Ploem, J.S.: The use of a vertical illuminator with interchangable dichroic mirrors for fluorescence microscopy with incident light. Z. wiss. Mikr. 68, 129–142 (1967)

  20. Ruch, F., Leemann, U.: Cytofluorometry. In: Micromethods in molecular biology (V. Neuhoff, ed.), p. 329–346. Berlin, Heidelberg, New York: Springer 1973

  21. Russell, W.C., Newman, C., Williamson, D.H.: A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses. Nature (Lond.) 253, 461–462 (1975)

  22. Thiébaud, C.H.: Quantitative determination of amplified rDNA and its distribution during oogenesis in Xenopus laevis. Chromosoma (Berl.) 73, 37–44 (1979)

  23. Thomas, C., Schram, A.: Correlation between the condensation state of rDNA and rRNA synthesis during Xenopus laevis oogenesis. Biol. Cellulaire 30, 49–54 (1977)

  24. Van Gansen, P., Schram, A.: Ultrastructure et cytochimie ultrastructurale de la vésicule germinative et du cytoplasme périnucléaire de l'ovocyte mûr de Xenopus laevis. J. Embryol. exp. Morph. 20, 375–390 (1968)

  25. Van Gansen, P., Schram, A.: Evolution of the nucleoli during oogenesis in Xenopus laevis studied by electron microscopy. J. Cell Sci. 10, 339–367 (1972)

  26. Watson-Coggins, L., Gall, J.G.: The timing of meiosis and DNA synthesis during early oogenesis in the toad. Xenopus laevis. J. Cell Biol. 52, 569–576 (1972)

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Thiébaud, C.H. The intra-nucleolar localization of amplified rDNA in Xenopus laevis oocytes. Chromosoma 73, 29–36 (1979). https://doi.org/10.1007/BF00294841

Download citation

Keywords

  • Developmental Biology
  • Internal Structure
  • Xenopus Laevis
  • Circular Form
  • Xenopus Laevis Oocyte