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Transforming growth factor β-like activity in human hydrocele fluid

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Summary

In this study transforming growth factorβ (TGF-β)-like activity in human hydrocele fluid was investigated. Inhibition of DNA synthesis of adult rat hepatocytes in primary culture and stimulation of colony formation of normal rat kidney (NRK) fibroblasts, clone 49F in soft agar were observed in all acidified hydrocele fluids and these activites were neutralized by the specific antibody raised against human native TGF-β. In samples obtained from recurrent cases of hydrocele, TGFβ-like activity was observed in its active form (without acidification). These results suggest that human hydrocele fluid contains TGFβ-like activity and that the active form of TGF-β in recurrent hydrocele fluid may be responsible for the recurrence of the disease even after repeated aspiration.

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References

  1. Carr BI, Hyashi I, Branum EL, Moses HL (1986) Inhibition of DNA synthesis in rat hepatocytes by platelet derived type-β transforming growth factor. Cancer Res 46:2330

    Google Scholar 

  2. DeLarco JE, Todaro GJ (1978) Growth factors from murine sarcoma virus-transformed cells. Proc Natl Acad Sci USA 75:4001

    Google Scholar 

  3. Fava R, Olsen N, Oja K, Moses H, Pincus T (1989) Active and latent forms of transforming growth factor β activity in synovial effusions. J Exp Med 169:291

    Google Scholar 

  4. Hirai R, Yamaoka K, Mitsui H (1983) Isolation and partial purification of a new class of transforming growth factors from an avian sarcoma virus transformed cell line. Cancer Res 43:5742

    Google Scholar 

  5. Huggins CB, Entz FH (1931) Absorption from normal tunica vaginalis testis, hydrocele and spermatocele. J Urol 25:447

    Google Scholar 

  6. Lyons MR, Oja JK, Moses HL (1988) Proteolytic activation of latent transfroming growth factor-β from fibroblast-conditioned medium. J Cell Biol 106:1659

    Google Scholar 

  7. Miyazono K, Heldin CH (1989) Role of carbodydrate structures in TGF-β1 latency. Nature 338:158

    Google Scholar 

  8. Miyazono K, Hellman U, Wernstedt C, Heldin CH (1988) Latent high molecular weight complex of transforming growth factor β1 purification from human platelets and structural characterization. J Biol Chem 263:6407

    Google Scholar 

  9. Moses HL, Tucker RF, Leof EB, Coffey RJ, Halper TrJ, Shipley GD (1985) Type-β transforming growth factor is a growth stimulator and a growth inhibitor. Cancer cells 3/ Growth factor and Transformation. Cold Spring Harbor Lab., New York, p 65

    Google Scholar 

  10. Moses HL, Coffey RJ, Leof EB, Lyons RM, Keski-Oja J (1987) Transforming growth factor β regulation of cell proliferation. J Cell Physiol [Suppl] 5:1

    Google Scholar 

  11. Nakamura T, Tomita Y, Hirai R, Yamaoka K, Kaji K, Ichihara A (1985) Inhibitory effect of transforming growth factor-β on DNA synthesis of adult rat hepatocytes in primary culture. Biochem Biophys Res Commun 133:1042

    Google Scholar 

  12. Nakamura T, Teramoto H, Tomita Y, Ichihara A (1986) Two types of growth inhibitor in rat platelets for primary cultured rat hepatocytes. Biochem Biophys Res Commun 134:755

    Google Scholar 

  13. Nakamura T, Arakaki R, Ichihara A (1988) Interleukin-1β is a potent growth inhibitor of adult rat hepatocytes in primary culture. Exp Cell Res 179:488

    Google Scholar 

  14. Owens GK, Geisterfer AAT, Young YWH, Komoriya A (1988) Transforming growth factor-β induced growth inhibition and cellular hypertrophy in cultured vascular smooth muscle cells. J Cell Biol 107:771

    Google Scholar 

  15. Pircher R, Lawrence DA, Jullien P (1984) Latent β-transforming growth factor in nontransformed and kirsten sarcoma virustransformed normal rat kidney cells, clone 49F. Cancer Res 44:5538

    Google Scholar 

  16. Ranganathan G, Lyons R, Jiang NS, Moses H (1987) Transforming growth factor type β in normal human urine. Biochem Biophys Res Commun 148:1503

    Google Scholar 

  17. Roberts AB, Frolik CA, Anzano MA, Sporn MB (1983) Transforming growth factors from neoplastic and nonneoplastic tissues. Fed Proc 42:2621

    Google Scholar 

  18. Sairenji M, Suzuki K, Murakami K, Motohashi H, Okamoto T, Umeda M (1987) Transforming growth factor activity in pleural and peritoneal effusions from cancer and non-cancer patients. Jpn J Cancer Res 78:814

    Google Scholar 

  19. Savage CR, Cohen S (1972) Epidermal growth factor and a new derivative. Rapid isolation procedures and biological and chemical characterization. J Biol Chem 247:7609

    Google Scholar 

  20. Seo MK, Lynch KE, Podolsky DK (1988) Multiplicity of transforming growth factors in human malignant effusions. Cancer Res 48:1792

    Google Scholar 

  21. Sporn MB, Roberts AB, Wakefield LM, Assoian RK (1986) Transforming growth factor-β: biological function and chemical structure. Science 233:532

    Google Scholar 

  22. Tanaka K, Sato M, Tomita Y, Ichihara A (1978) Biochemical studies on liver functions in primary culture hepatocytes of adult rats. Hormonal effects on cell viability and protein synthesis. J Biochem [Tokyo] 84:937

    Google Scholar 

  23. Twarzik DR, Sherwin SA, Ranchalis J, Todaro GJ (1982) Transforming growth factors in urine of normal, pregnant and tumor-bearing humans. JNCI 69:793

    Google Scholar 

  24. Wakefield LM, Smith DM, Masui T, Harris CC, Sporn MB (1987) Distribution and modulation of the cellular receptors for transforming growth factor-beta. J Cell Biol 105:965

    Google Scholar 

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Authors and Affiliations

  1. Department of Urology, Nagasaki University School of Medicine, Nagasaki, Japan

    P. K. Saha, S. Kanda, H. Morimitsu, N. Nishimura, H. Kanetake & Y. Saito

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  1. P. K. Saha
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  2. S. Kanda
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  3. H. Morimitsu
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  4. N. Nishimura
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  5. H. Kanetake
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  6. Y. Saito
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Saha, P.K., Kanda, S., Morimitsu, H. et al. Transforming growth factor β-like activity in human hydrocele fluid. Urol. Res. 18, 295–298 (1990). https://doi.org/10.1007/BF00294778

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  • DOI: https://doi.org/10.1007/BF00294778

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