Advertisement

Springer Nature is making Coronavirus research free. View research | View latest news | Sign up for updates

Immunohistological investigation of mononuclear cell infiltrates in meningiomas

  • 33 Accesses

  • 21 Citations

Summary

Immunohistochemical analysis of inflammatory cell density and infiltrate subpopulations in 42 meningiomas was performed. Evaluation of infiltrating cell density was carried out by cell counting. Meningothelial and fibroblastic meningiomas contained an average of 3% mononuclear cells; the few lymphocytes were localized in the perivascular spaces. In subtypes with cellular atypies and recurrent tumors, the inflammatory cells increased up to 9%. We found small mononuclear cell clusters in the tumor parenchyma in addition to the perivascular infiltrates. Marked degrees of infiltration were found in anaplastic meningiomas (average 13.5% of total cells). The lymphocytic infiltrates were localized in multilayered perivascular cuffings and intraparenchymal cell clusters. The composition of the infiltrates, i.e., predominantly a mixed staining of cytotoxic/suppressor and helper cell phenotypes, did not vary in the different subtupes. We conclude: (1) that inflammatory infiltration is more frequent and denser in malignant than in benign meningiomas; and (2) that the tumor defense mechanisms in meningiomas are mediated particularly by T cell mediated immunity.

This is a preview of subscription content, log in to check access.

References

  1. 1.

    Baumgartner I, Scheiner O, Holzinger C, Boltz-Nitulescu G, Klech H, Lassmann H, Rumpold H, Förster O, Kraft D (1988) Expression of the VEP13 Antigen (CD16) on native human alveolar macrophages and cultured blood monocytes. Immunobiology 177: 317–326

  2. 2.

    Bernard A, Bournsell L, Dausset J, Milstein C, Schlossman SF (1984) Leukocyte typing, Proc 1st Intern Workshop Leukocyte Differentiation Antigens. Springer, Berlin Heidelberg New York, pp 148–157

  3. 3.

    Bertrand I, Mannen H (1960) Etudes des réactions vasculairés dans les astrocytomes. Rev Neurol (Paris) 102: 3–19

  4. 4.

    Böker DK, Kalff R, Gulotta F (1984) Mononuclear infiltrates in human intracranial tumors as a prognostic factor. Influence of preoperative steroid treatment. Clin Neuropathol 3: 143–147

  5. 5.

    Brooks WH, Netsky MG, Normansell DE (1972) Depressed cell-mediated immunity in patients with primary intracranial tumors. J Exp Med 136: 1631–1647

  6. 6.

    Brooks WH, Caldwell HD, Mortara RH (1974) Immune response in patients with gliomas. Surg Neurol 2: 419–423

  7. 7.

    Brooks WH, Markesbery WR, Gupta GD (1978) Relationship of lymphocyte invasion and survival of brain and tumor patients. Ann Neurol 4: 219–224

  8. 8.

    Cordell JL, Falini B, Erber WN (1984) Immunoenzymatic labelling of monoclonal anti-alkaline phosphatase. J Histochem Cytochem 32: 319–329

  9. 9.

    Hanwehr RI von, Hofman FM, Taylor CR (1984) Mononuclear lymphoid populations infiltrating the microenvironment of primary CNS tumours. J Neurosurg 60: 1138–1147

  10. 10.

    Hoog N, Horton MA (1987) Myeloid antigens: new and previously defined clusters. In: McMichel AJ (ed) Leukocyte typing III. Oxford University Press, Oxford, p 576

  11. 11.

    Horten BC, Urich H, Stefoski D (1979) Meningiomas with conspicuous plasma cell-lymphocytic components. A report of five cases. Cancer 43: 258–364

  12. 12.

    Hsu SM, Raine L, Fanger H (1981) The use of avidin-biotinperoxidase complexi(ABC) in immunoperoxidase techniques: a comparison between ABC and unlabelled antibody (PAP) procedures. J Histochem Cytochem 29: 577–580

  13. 13.

    Kepes JJ (1982) Meningiomas. Biology, pathology, and differential diagnosis. In: Kepes JJ (ed) Masson monographs in diagnostic pathology. Masson, New York, pp 111–112

  14. 14.

    Kril MP, Apuzzo LJ (1983) Observations in the study of T lymphocyte subsets by monoclonal antibodies and flow cytometric analysis in intracranial neoplastic disorders. Clin Neurosurg 30: 125–136

  15. 15.

    McMichael LJ, Fabre J (1982) Monoclonal antibodies in clinical medicine. Academic Press, London, pp 62–75

  16. 16.

    Meyer-Rienecker HJ, Jenssen HL, Mix E (1985) Heterogene Immunreaktivität bei Hirntumoren: Ergebnisse zur zellulären Immunität gegenüber Hirngewebsantigenen. Fortschr Neurol Psychiatr 53: 363–369

  17. 17.

    Mirra SS, Tindall SC, Check IJ, Brynes RK, Moore WW (1983) Inflammatory meningeal masses of unexplained origin. An ultrastructural and immunological study. J Neuropathol Exp Neurol 42: 453–468

  18. 18.

    Mork SJ, Nyland H, Matre R, Ganz J (1985) Characterization of host mononuclear cells in gliomas. J Neuropathol Exp Neurol 44: 317 [abstr]

  19. 19.

    Palma L, Di Lorenzo N, Guidetti B (1978) Lymphocytic infiltrates in primary glioblastomas and recidivous gliomas. J Neurosurg 49: 854–861

  20. 20.

    Perentes E, Rubinstein LJ (1986) Immunohistochemical recognition of human neuroepithelial tumors by Anti-Leu-7 (HNK-1) monoclonal antibody. Acta Neuropathol (Berl) 69: 227–233

  21. 21.

    Phillips JP, Eremin O, Anderson JR (1982) Lymphoreticular cells in human brain tumours and in normal brain. Br J Cancer 45: 61–69

  22. 22.

    Reinherz EL, Haynes B, Nadler L, Bernstein I (1986) Leukocyte typing II. Proc 2nd Intern Workship Leukocyte Differentiation Antigens. Springer, Berlin Heidelberg New York, pp 189–197

  23. 23.

    Ridley A, Cavanagh JB (1971) Lymphocytic infiltration in gliomas: evidence of possible host resistance. Brain 94: 117–124

  24. 24.

    Roggendorf W, Schuster T, Peiffer J (1987) Proliferative potential of meningiomas determined with the monoclonal antibody Ki 67. Acta Neuropathol (Berl) 73: 361–364

  25. 25.

    Roitt IM, Brostoff J, Male DK (1985) Immunology. Gower Medical Publishing, London New York, pp 4.8–4.11

  26. 26.

    Rossi ML, Hughes JT, Esiri MM, Coakham HB, Brownell DB (1987) Immunohistological study of mononuclear cell infiltrate in malignant gliomas. Acta Neuropathol (Berl) 74: 269–277

  27. 27.

    Russell DS, Rubinstein LJ (1977) Pathology of tumours of the nervous system. Williams & Wilkins, Baltimore, pp 330–335

  28. 28.

    Schiffer D, Croveri G, Pautasso C (1974) Frequenza e significato degli infiltrati linfo-plasmacellulari nei gliomi umani. Tumori 60: 177–184

  29. 29.

    Stam FC, van Alphen HAM, Boorsma DM (1980) Meningioma with conspicuous plasma cell componet. A histopa-thological and immunohistochemical study. Acta Neuropathol (Berl) 49: 241–243

  30. 30.

    Stavrou D, Anzil AP, Weidenbach W, Rodt H (1977) Immunofluorescence study of lymphocytic infiltration in gliomas. Identification of T lymphocytes. J Neurol Sci 33: 275–282

  31. 31.

    Stevens A, Klöter I, Roggendorf W (1988) Inflammatory infiltrates and NK cells presence in human brain tumors. Cancer 61: 738–743

  32. 32.

    Takakura K, Sing OK (1972) Inhibitory role of immunologically competent cells on glioblastoma and its clinical application: in vitro studies. In: Fusek J, Kunc Z (eds) Present limits of neurosurgery. Excerpta Medica, Amsterdam, pp 47–51

  33. 33.

    Takeuchi J, Barnard RO (1976) Perivascular lymphocytic cuffing in astrocytomas. Acta Neuropathol (Berl) 35: 265–271

  34. 34.

    West SG, Pittman DL, Coggin JT (1980) Intracranial plasma cell granuloma. Cancer 46: 330–335

  35. 35.

    Wood GHW, Morantz RA (1979) Immunohistologic evaluation of the lymphoreticular infiltrate of human central nervous system tumours. J Natl Canc Inst 62: 485–491

  36. 36.

    Wood GW, Morantz RA, Tilzer SA (1980) Immunoglobulin bound in vivo to Fc receptor-positive cells in human central nervous system tumours. J Natl Canc Inst 64: 411–418

  37. 37.

    Young HF, Sakales R, Kaplan AM (1976) Inhibition of cell-mediated immunity in patients with primary intracranial tumors. Surg Neurol 5: 19–23

Download references

Author information

Correspondence to I. Becker.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Becker, I., Roggendorf, W. Immunohistological investigation of mononuclear cell infiltrates in meningiomas. Acta Neuropathol 79, 211–216 (1989). https://doi.org/10.1007/BF00294381

Download citation

Key words

  • Meningioma
  • Mononuclear infiltration
  • Lymphocyte subset
  • Tumor immunology