The recruitment of monocytes into the scrapie-affected brain was investigated in female mice reconstituted with male bone marrow, using a Y-chromosome-specific probe and F4/80 immunocytochemistry. Recruitment of monocytes could be demonstrated in six out of eight animals and the number of recruited cells correlated with the severity of vacuolation in most, but not all, animals. The proportion of microglia derived from recruited monocytes varied between individual animals, did not correlate with the increase in cellularity (glia) in affected areas of brain and did not affect the length of incubation period. Thus, it is unlikely that the recruitment of monocytes is a pivotal event in the development of early pathological changes in scrapie. The morphology of recruited cells in scrapie lesions, as revealed by F4/80 immunoreactivity, was indistinguishable from that of activated resident microglia.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Bishop CE, Boursot P, Baron B, Bonhomme F, Hatat D (1985) Most classical Mus musculus domesticus laboratory mouse strains carry a Mus musculus musculus Y chromosome. Nature 315: 70–72
Bruce ME, Fraser H (1975) Amyloid plaques in the brains of mice infected with scrapie: morphological variation and staining properties. Neuropathol Appl Neurobiol 1: 189–202
Bruce ME, Fraser H (1981) Effect of route of infection on the frequency and distribution of cerebral amyloid plaques in scrapie mice. Neuropathol Appl Neurobiol 7: 289–298
Bruce ME, Dickinson AG, Fraser H (1976) Cerebral amyloidosis in scrapie in the mouse: effect of agent strain and mouse genotype. Neuropathol Appl Neurobiol 2: 471–478
Bruce ME, McBride PA, Farquhar CF (1989) Precise targeting of the pathology of the sialoglycoprotein, PrP, and vacuolar degeneration in mouse scrapie. Neurosci Lett 102: 1–6
DeArmond SJ, Mobley WC, DeMott DL, Barry RA, Beckstead JH, Prusiner SB (1987) Changes in the localization of brain prion proteins during scrapie infection. Neurology 37: 1271–1280
Diedrich JF, Minnigan H, Carp RI, Whitaker JN, Race R, Frey II W, Haase AT (1991) Neuropathological changes in scrapie and Alzheimer's disease are associated with increased expression of apolipoprotein E and cathepsin D in astrocytes. J Virol 65: 4759–4768
Fraser H (1976) The pathology of natural and experimental scrapie. In: Kimberlin RH (ed) Slow virus diseases of animals and man. North Holland, Amsterdam, pp 267–305
Fraser H (1978) Scrapie: a transmissible degenerative CNS disease. In: Behan PO, Rose, FC (eds) Progress in neurological research. Pitman, London, pp 194–210
Fraser H, Dickinson AG (1973) Agent-strain differences in the distribution and intensity of grey matter vacuolation. J Comp Pathol 83: 29–40
Hadlow WJ (1961) The pathology of experimental scrapie in the dairy goat. Res Vet Sci 2: 289–314
Hecker R, Taraboulos A, Scott M, Pan K-M, Yang S-L, Torchia M, Jendroska K, DeArmond SJ, Prusiner SB (1992) Replication of distinct scrapie prion isolates is region specific in brains of transgenic mice and hamsters. Genes Dev 6: 1213–1228
Hickey WF, Vass K, Lassmann H (1992) Bone marrow-derived elements in the central nervous system: an immunohisto-chemical and ultrastructural study of rat chimeras. J Neuropathol Exp Neurol 51: 246–256
Ironside JW, Barrie C, McCardle L, Bell JE (1993) Microglial cell reactions in human spongiform encephalopathies (abstract). Neuropathol Appl Neurobiol 19: 203
Lafarga M, Berciano MT, Suarez I, Viadero CR, Andres MA, Berciano J (1991) Cytology and organization of reactive astroglia in human cerebellar cortex with severe loss of granule cells: a study on the ataxic form of Creutzfeldt-Jakob disease. Neuroscience 40: 337–352
Lassmann H, Schmied M, Vass K, Hickey WK (1993) Bone marrow-derived elements and resident microglia in brain inflammation. Glia 7: 19–24
Liberski PP (1987) Astrocytic reaction in experimental scrapie in hamsters. J Comp Pathol 97: 73–78
Liberski PP, Nerurkar VR, Yanagihara R, Gajdusek DC (1990) Tumor necrosis factor: cytokine-mediated myelin vacuolation in experimental Creutzfeldt-Jakob Disease. Proceedings of VI-IIth International Congress of Virology, Berlin, August 24–26, 1990. International Union of Microbiological Societies, abstr. no. P69-015: 421
McBride PA, Bruce ME, Fraser H (1988) Immunostaining of scrapie cerebral amyloid plaques with antisera raised to scrapie-associated fibrils (SAF). Neuropathol Appl Neurobiol 14: 325–336
McLean IW, Nakane NK (1974) Periodate-lysine-paraform-aldehyde fixative. A new fixative for immunoelectron microscopy. J Histochem Cytochem 22: 1077–1083
Miyazono M, Iwaki T, Kitamoto T, Kaneko Y, Doh-ura K, Tateishi J (1991) A comparative immunohistochemical study of kuru and senile plaques with a special reference to glial reactions at various stages of amyloid plaque formation. Am J Pathol 139: 589–598
Perry VH, Hune DA, Gordon S (1985) Immunohistochemical localization of macrophages and microglia in the adult and developing mouse brain. Neuroscience 15: 313–326
Ryder S (1993) Studies on the recruitment of macrophages into the central nervous system. PhD Thesis, University of Cambridge, Cambridge
Scott JR, Fraser H (1984) Degenerative hippocampal pathology in mice infected with scrapie. Acta Neuropathol (Berl) 65: 62–68
Williams AE, Lawson LJ, Perry VH, Fraser H (1994) Characterisation of the microglial response in murine scrapie. Neuropathol Appl Neurobiol 20: 47–55
Williams AE, van Dam A-M, Berkenbosch F, Eikelenboom P, Fraser H (1994) Cytokines, prostaglandins and lipocortin-1 are present in the brains of scrapie-infected mice. Neurobiol Aging 15 (Suppl 1): S89
Williams AE, van Dam A-M, Man-A-Hing WKH, Berkenbosch F, Eikelenboom P, Fraser H (1994) Cytokines, prostaglandins and lipocortin-1 are present in the brains of scrapie-infected mice. Brain Res 654: 200–206
About this article
Cite this article
Williams, A.E., Ryder, S. & Blakemore, W.F. Monocyte recruitment into the scrapie-affected brain. Acta Neuropathol 90, 164–169 (1995). https://doi.org/10.1007/BF00294316
- Central nervous system