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Tumorous cultures of Crepis capillaris: chromosomes and growth

Abstract

Different crown-gall culture strains of Crepis capillaris derived from haploid and diploid tumors were studied karyologically during the period of 18 transfers (18–20 months). The very low proportion of mitotic anomalies observed in the first transfers is in agreement with the generally accepted assumption that chromosomal changes are not involved in the initiation of tumors. However, it has been established that an intimate relationship exists between chromosome aberrations and the further development of the tumors. Morphological and physiological changes which occurred in some strains and substrains were invariably associated with cytologieal changes. The results are discussed in connection with similar phenomena described for animal tumors.

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References

  1. Bloch-Shtacher, N., Rabinowitz, Z., Sachs, L.: Chromosomal mechanism for the induction of reversion in transformed cells. Int. J. Cancer 9, 632–640 (1972)

  2. Braun, A. C.: A demonstration of the recovery of the crown-gall tumor cell with the use of complex tumors of single-cell origin. Proc. nat. Acad. Sci. (Wash.) 45, 932–938 (1959)

  3. Hitosumachi, S., Rabinowitz, Z., Sachs, L.: Chromosomal control of reversion in transformed cells. Nature (Lond.) 231, 511–514 (1971)

  4. Kirkland, J. A., Stanley, M. A.: Chromosomes of cancer cells. Nature (Lond.) 232, 632–633 (1971)

  5. Koller, P. C.: The role of chromosomes in cancer biology, 122 pp. Berlin-Heidelberg-New York: Springer 1972

  6. Melchers, G.: Transformation or habituation to autotrophy and tumor growth and recovery. Colloques internat. C. N. R. S. No. 193, 229–234, Paris 1971

  7. Mitelman, F.: The chromosomes of fifty primary Rous rat sarcomas. Hereditas (Lund) 69, 155–186 (1971)

  8. Muldal, S., Elejalde, R., Harwey, P. W.: Specific chromosome anomaly associated with autonomous and cancerous development in man. Nature (Lond.) 229, 48–49 (1971)

  9. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plantarum 15, 473–497 (1962)

  10. Pollack, R., Wolman, S., Vogel, A.: Reversion of virus-transformed cell lines: hyperploidy accompanies retention of viral genes. Nature (Lond.) 228, 938–970 (1970)

  11. Sacristán, M. D.: Karyotypic changes in callus cultures from haploid and diploid plants of Crepis capillaris (L.) Wallr. Chromosoma (Berl.) 33, 273–283 (1971)

  12. Sacristán, M. D., Melchers, G.: The caryological analysis of plants regenerated from tumorous and other callus cultures of tobacco. Molec. gen. Genet. 105, 317–333 (1969)

  13. Sacristán, M. D., Wendt-Gallitelli, M. F.: Transformation to auxin-autotrophy and its reversibility in a mutant line of Crepis capillaris callus culture. Molec. gen. Genet. 110, 355–360 (1971)

  14. Wiener, F., Klein, G., Harris, H.: Chromosomal control of malignancy. Colloque Protoplastes, Versailles 1972

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Sacristán, M.D., Wendt-Gallitelli, M.F. Tumorous cultures of Crepis capillaris: chromosomes and growth. Chromosoma 43, 279–288 (1973). https://doi.org/10.1007/BF00294276

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Keywords

  • Developmental Biology
  • Physiological Change
  • Intimate Relationship
  • Chromosome Aberration
  • Chromosomal Change