A 2.2 kilobase (kb) fragment of ColIdrd-1 cloned in pBR325 causes phage T5 and BF23 infections of Escherichia coli K-12 to abort. This abortive infection is associated with leakage of β-galactosidase from the cell. A recombinant plasmid containing a 2.8 kb fragment of ColIdrd-1 encodes colicin Ib but fails to cause abortive infection. Tn5 and Tn10 insertions into ColIdrd-1 that abolish colicin Ib production have no effect on the abortive infection phenotype. These findings are inconsistent with a previously proposed role for colicin Ib in causing phage infections to abort.
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Communicated by B.A. Bridges
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Boulnois, G.J. Colicin Ib does not cause plasmid-promoted abortive phage infection of Escherichia coli K-12. Molec. Gen. Genet. 182, 508–510 (1981). https://doi.org/10.1007/BF00293944
- Escherichia Coli
- Recombinant Plasmid
- Phage Infection
- Tn10 Insertion
- Abortive Infection