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Characterization of Parasarcophaga heterochromatin

I. Mitotic chromosomes

Abstract

The heterochromatin of neuroblast metaphase chromosomes in six sympatric species of Parasarcophaga was characterized by C-, N-, quinacrine (Q)-, Hoechst 33258 (H)-, Hoechst/actinomycin D (H/AMD)- and acridine orange (AO)-banding. The autosomes and the sex chromosomes in all the six species are characterized by the presence of C- and N-positive heterochromatin. The N-bands lie within the limits of the C+ heterochromatin. AT-specific fluorochromes further differentiate heterochromatin into Q+H+,QH+ and QH types and the repetitive nature of these regions is indicated by their fast reassociating nature with AO-staining. Thus, on the basis of these results, the heterochromatin of mitotic chromosomes in Parasarcophaga can be classified into C+N+Q+H+, C+N+QH, c+nqh and CNQH+ types.

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Correspondence to Dipika Kaul.

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Kaul, D., Gaur, P., Agrawal, U.R. et al. Characterization of Parasarcophaga heterochromatin. Chromosoma 98, 49–55 (1989). https://doi.org/10.1007/BF00293334

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Keywords

  • Developmental Biology
  • Acridine
  • Acridine Orange
  • Metaphase Chromosome
  • Mitotic Chromosome