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Characterization of Parasarcophaga heterochromatin

I. Mitotic chromosomes


The heterochromatin of neuroblast metaphase chromosomes in six sympatric species of Parasarcophaga was characterized by C-, N-, quinacrine (Q)-, Hoechst 33258 (H)-, Hoechst/actinomycin D (H/AMD)- and acridine orange (AO)-banding. The autosomes and the sex chromosomes in all the six species are characterized by the presence of C- and N-positive heterochromatin. The N-bands lie within the limits of the C+ heterochromatin. AT-specific fluorochromes further differentiate heterochromatin into Q+H+,QH+ and QH types and the repetitive nature of these regions is indicated by their fast reassociating nature with AO-staining. Thus, on the basis of these results, the heterochromatin of mitotic chromosomes in Parasarcophaga can be classified into C+N+Q+H+, C+N+QH, c+nqh and CNQH+ types.

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  1. Agrawal UR (1987) Chromosomal studies in Sarcophagidae with special reference to nature and function of heterochromatin. Ph D thesis, University of Allahabad, India

  2. Agrawal UR, Kaul D (1988) Polytene chromosome maps of Parasarcophaga albiceps (Sarcophagidae:Diptera). In: Bhatnagar VS (ed) Advances in cytology and genetics. Allahabad Univ, Allahabad, pp 76–86

  3. Boyes JW, van Brink JM (1965) Chromosomes of Calyptrate Diptera. Can J Genet Cytol 7:537–550

  4. Cardoso H, Saez FA, Brum-Zorrilla N (1974) Location, structure and behaviour of C-heterochromatin during meiosis in Dichroplus silveiraguidoi (Acrididae:Orthoptera). Chromosoma 48:51–64

  5. Das BC, Raman R, Sharma T (1979) Chromosome condensation and Hoechst 33258 fluorescence in meiotic chromosomes of the grasshopper Spathosternum prasiniferum (Walker). Chromosoma 70:251–258

  6. Dev V, Lachance LE, Whitten CJ (1986) Polytene chromosomes karyotype correlations and population cytology of the primary screwworm fly. J Hered 77:427–434

  7. Faust J, Vogel W (1974) Are ‘N Bands’ selective staining of specific heterochromatin? Nature 249:352–353

  8. Foster GG, Whitten MJ, Konovalov C, Bedo DG, Maddern RH, Boon DJ (1980) Cytogenetic studies in Lucilia cuprina dorsalis R.-D. (Diptera:Calliphoridae). Polytene chromosome maps of the autosomes and cytogenetic localization of visible genetic markers. Chromosoma 81:151–168

  9. Funaki K, Matsui S, Sasaki M (1975) Location of nucleolar organizers in animal and plant chromosomes by means of an improved N-banding technique. Chromosoma 49:357–370

  10. Gaur P (1983) Cytogenetic studies in Sarcophagidae. Ph D thesis, University of Allahabad, India

  11. Gaur P, Agrawal UR, Tewari RR, Kaul D (1984) Heterogeneity of the sex chromosome heterochromatin in the genus Parasarcophaga (Sarcophagidae:Diptera). In: Seno S, Okada Y (eds) International Cell Biology 1984: 3rd Int Congr Cell Biol abstr, Tokyo, p 234

  12. Gaur P, Agrawal UR, Tewari RR, Kaul D (1985) C- and Q-band polymorphism in the mitotic chromosomes of flesh-flies belonging to the genus Parasarcophaga (Sarcophagidae: Diptera). La Kromosomo II-39-40:1207–1213

  13. Hagele K (1977a) Differential staining of polytene chromosome bands in Chironomus by Giemsa banding methods. Chromosoma 59:207–216

  14. Hagele K (1977b) N-banding in polytene chromosomes of Chironomus and Drosophila. Chromosoma 63:78–88

  15. Hagele K (1979a) Characterization of heterochromatin in Schistocerca gregaria by C-and N-banding methods. Chromosoma 70:239–250

  16. Hägele K (1979b) Selective staining of X chromosome segments in Schistocerca gregaria after denaturation and reassociation procedures. Chromosoma 71:217–225

  17. Jorgenson KF, Sande JH van de, Lin CC (1978) The use of base pair specific DNA binding agents as affinity labels for study of mammalian chromosomes. Chromosoma 68:287–302

  18. Kaul D, Tewari RR (1983) Interspecific comparisons of polytene chromosomes in the genus Parasarcophaga (Sarcophagidae:Diptera). Genetica 62:129–138

  19. Kaul D, Chaturvedi R, Gaur P, Tewari RR (1978) Cytogenetics of the genus Parasarcophaga (Sarcophagidae:Diptera). Chromosoma 68:73–82

  20. Lee CS, Collins L (1977) Q-and C-bands in the metaphase chromosomes of Drosophila nasutoides. Chromosoma 61:57–60

  21. Matsui S (1974a) Nucleolus organizer of Vicia faba chromosomes revealed by the N-banding technique. Jpn J Genet 49:93–96

  22. Matsui S (1974b) Structural proteins associated with ribosomal cistrons in Xenopus laevis chromosomes. Exp Cell Res 88:88–94

  23. Matsui S, Sasaki M (1973) Differential staining of nucleolar organizers in mammalian chromosomes. Nature 246:148–150

  24. Pimpinelli S, Santini G, Gatti M (1976) Characterization of Drosophila heterochromatin. II. C- and N-banding. Chromosoma 57:377–387

  25. Tewari RR (1988) Karyotypic evolution in Calyptrate Diptera. In: Bhatnagar VS (ed) Advances in cytology and genetics. Allahabad Univ, Allahabad, pp 97–114

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Correspondence to Dipika Kaul.

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Kaul, D., Gaur, P., Agrawal, U.R. et al. Characterization of Parasarcophaga heterochromatin. Chromosoma 98, 49–55 (1989).

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  • Developmental Biology
  • Acridine
  • Acridine Orange
  • Metaphase Chromosome
  • Mitotic Chromosome