Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Inheritance of chromosomal length polymorphisms in the ascomycete Leptosphaeria maculans

Abstract

Pulsed field gel electrophoresis experiments show that chromosomal length polymorphisms are produced during meiosis in the ascomycetous plant pathogen Leptosphaeria maculans. Homologues in tetrads of L. maculans were identified on the basis of their binding to chromosome-specific probes that included β-tubulin, nitrate reductase, 18S ribosomal DNA and two Random Amplified Polymorphic DNA (RAPD) markers. Changes in size of homologues were followed during meiosis. Significant karyotype variation was evident due to the random assortment of parental homologues of different sizes. In most cases, the progeny had the same-sized homologues as the parents; however, in some instances novel-sized homologues were detected that varied in size from those of the parents by up to 50 kb. Our results are consistent with the hypothesis that these novel chromosomal length polymorphisms are produced by reciprocal recombination between parental homologous chromosomes of unequal sizes.

This is a preview of subscription content, log in to check access.

References

  1. Howlett BJ, Brownlee AG, Guest DI, Adcock G, McFadden GI (1992) The 5S ribosomal RNA gene is linked to large and small subunit ribosomal RNA genes in several oomycetes. Curr Genet 22:455–461

  2. Kinscherf TG, Leong SA (1988) Molecular analysis of the karyotype of Ustilago maydis. Chromosoma 96:427–433

  3. Koenraadt H, Somerville SC, Jones AL (1992) Characterisation of mutations in the β-tubulin gene of benomyl-resistant field strains of Venturia inaequalis and other plant pathogenic fungi. Phytopathology 82:1348–1354

  4. Mengistu A, Rimmer RS, Williams PH (1993) Protocols for in vitro sporulation, ascospore release, sexual mating and fertility in crosses of Leptosphaeria maculans. Plant Dis 77:538–540

  5. Mengistu A, Hill CB, Williams PH (1995) An improved method for mating and fertility in crosses of Leptosphaeria maculans, the causal agent of blackleg of crucifers. Plant Dis, in press

  6. Morales VM, Seguin-Swartz G, Taylor JL (1993) Chromosome length polymorphism in Leptosphaeria maculans. Phytopathology 83:503–509

  7. Ono B, Ishino-Aran Y (1988) Inheritance of chromosomal length polymorphisms in Saccharomyces cerevisiae. Curr Genet 14:413–418

  8. Orbach MJ, Porro EB, Yanofsky C (1986) Cloning and characterization of the gene for β tubulin from a benomyl resistant mutant of Neurospora crassa and its use as a dominant selectable marker. Mol Cell Biol 6:2452–2461

  9. Plummer KM, Howlett BJ (1993) Major chromosomal length polymorphisms are evident after meiosis in the blackleg fungus, Leptosphaeria maculans. Curr Genet 24:107–113

  10. Powell WA, Kistler HC (1990) In vivo rearrangement of foreign DNA by Fusarium oxysporum produces linear self-replicating plasmids. J Bacteriol 172:3163–3171

  11. Russell BW, Mills D (1992) Electrophoretic karyotypes of Tilletia caries, T. controversa, and their F1 progeny: further evidence for conspecific status. Mol Plant Microbe Int 6:66–74

  12. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: A laboratory manual 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York

  13. Schechtman MG (1990) Characterisation of telomere DNA from Neurospora crassa. Gene 88:159–165

  14. Shampay J, Szostak JW, Blackburn EH (1984) DNA sequences of telomeres maintained in yeast. Nature 310:154–157

  15. Skinner DZ, Budde AD, Farman ML, Smith JR, Leong H, Leong SA (1993) Genome organization of Magnaporthe grisea: genetic map, electrophoretic karyotype, and occurrence of repeated DNAs. Theor Appl Genet 87:454–457

  16. Taylor JL, Borgmann IE (1994) Characterisation of an unusual sequence from the highly virulent strain of Leptosphaeria maculans and evidence of transfer of it to the weakly virulent strain. Mol Plant Microbe Int 7:181–188

  17. Taylor JL, Borgmann IE, Seguin-Swartz G (1991) Electrophoretic karyotyping of Leptosphaeria maculans differentiates highly virulent from weakly virulent isolates. Curr Genet 19:273–277

  18. Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535

  19. Williams RSB, Davis MA, Howlett BJ (1994) Nitrate reductase of Leptosphaeria maculans: gene sequence, chromosomal location, mutant isolation and complementation. Mol Gen Genet 244:1–8

  20. Zolan ME (1995) Chromosome-length polymorphisms in fungi. Microbiol Rev, in press

  21. Zolan ME, Heyler NK, Ramesh MA (1993) Gene mapping using marker chromosomes in Coprinus cinereus. In: Baltz RH, Hegeman GD, Skatrud PL (eds) Industrial organisms: basic and applied molecular genetics. American Society for Microbiology, Washington DC, pp 31–35

  22. Zolan ME, Heyler NK, Stassen NY (1994) Inheritance of chromosome-length polymorphisms in Coprinus cinereus. Genetics 137:87–94

Download references

Author information

Correspondence to Barbara J. Howlett.

Additional information

Communicated by C. van den Hondel

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Plummer, K.M., Howlett, B.J. Inheritance of chromosomal length polymorphisms in the ascomycete Leptosphaeria maculans . Molec. Gen. Genet. 247, 416–422 (1995). https://doi.org/10.1007/BF00293142

Download citation

Key words

  • Leptosphaeria maculans
  • Chromosomal length polymorphisms
  • Meiotic recombination
  • Tetrads