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High voltage electron microscopical analysis of chromosomal number in the slime mold Echinostelium minutum de bary

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Utilizing serial thick sectioning techniques in combination with high voltage electron microscopy and stereopair analysis a count of 124 chromosomes was determined for a metaphasic plasmodial nucleus of the slime mold E. minutum.

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  1. Aldrich, H.C.: Ultrastructure of meiosis in three species of Physarum. Mycologia 59, 127–148 (1967)

  2. Aldrich, H.C.: The ultrastructure of mitosis in myxamoebae and plasmodia of Physarum flavicomum. Amer. J. Bot. 56, 290–299 (1969)

  3. Aldrich, H.C., Mims, C.W.: Synaptonemal complexes and meiosis in Myxomycetes. Amer. J. Bot. 57, 935–941 (1970)

  4. Coss, R.A.: Mitosis in Chlamydomonas reinhardtii basal bodies and the mitotic apparatus. J. Cell Biol. 63, 325–329 (1974)

  5. Cosslett, V.E.: High-voltage electron microscopy. Quart. Rev. Biophys. 2, 95–133 (1969)

  6. Day, A.W.: Genetic implications of current models of somatic nuclear division in fungi. Canad. J. Bot. 50, 1337–1347 (1972)

  7. Day, A.W., Jones, J.K.: Somatic nuclear division in the sporidia of Ustilago violacea. I. Acetic orcein staining. Canad. J. Microbiol. 18, 663–670 (1972)

  8. Dupouy, G.: Electron microscopy at very high voltages. Adv. Opt. Elect. Microsc. 2, 167–250 (1968)

  9. Dupouy, G.: Three-megavolt electron microscopy. Endeavour 32, 66–70 (1973)

  10. Golomb, H.M., and Bahr, G.F.: Scanning electron microscopic observations of surface structure of isolated human chromosomes. Science. 171, 1024–1026 (1971)

  11. Hama, K., Porter, K.R.: An application of high voltage electron microscopy to the study of biological materials. High voltage electron microscopy. J. de Microsc. 8, 149–158 (1969)

  12. Harris, J.L.: Some three-dimensional aspects of Ceratocystis ulmi as observed by high-voltage electron microscopy. Mycologia 67, 332–341 (1975)

  13. Haskins, E.F.: The developmental biology of the eumycetozoan Echinostelium minutum. Ph.D. Dissertation, Univ. of Minnesota, Minneapolis. 1–105, 1965

  14. Haskins, E.F.: Developmental studies on the true slime mold Echinostelium minutum. Canad. J. Microbiol. 14, 1309–1315 (1968)

  15. Haskins, E.F.: Sporophore formation in the myxomycete Echinostelium minutum de Bary. Arch. Protistenk. 113, 123–130 (1971)

  16. Haskins, E.F.: Echinostelium minutum (Myxomycetes)—Amoebal phase. Film E 1816, Inst. wiss. Film, Göttingen1 1973a

  17. Haskins, E.F.: Echinostelium minutum (Myxomycetes)—Plasmodial phase (Protoplasmodium). Film E 1817, Inst. wiss. Film, Göttingen1 1973b

  18. Haskins, E.F., Hinchee, A.A., Cloney, R.A.: The occurrence of synaptonemal complexes in the slime mold Echinostelium minutum de Bary. J. Cell Biol. 51, 898–903 (1971)

  19. Luft, J.: Improvements in epoxy resin embedding medium. J. biophys. biochem. Cytol. 9, 409–414 (1961)

  20. Mohberg, M.J., Babcock, K.L., Haugli, F.B., Rusch, H.P.: Nuclear DNA content and chromosome numbers in the Myxomycete Physarum polycephalum. J. Cell Biol. 34, 228–245 (1973)

  21. Moses, M.J.: Synaptinemal complex. Annu. Rev. Genet. 2, 363–412 (1968)

  22. Moses, M.J.: Structure and function of the synaptonemal complex. Genetics 61, (Suppl.) 41–51 (1969)

  23. Paulin, J.J.: The chondriome of selected trypanosomatids. A three-dimensional study based on serial thick sections and high voltage electron microscopy. J. Cell Biol. 66, 404–413 (1975)

  24. Reynolds, E.S.: The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963)

  25. Ris, H.: Use of the high voltage electron microscope for the study of thick biological specimens. J. de Microsc. 8, 761–766 (1969)

  26. Robinow, C.F., Caten, C.E.: Mitosis in Aspergillus nidulans. J. Cell Sci. 5, 403–431 (1969)

  27. Ryser, U.: Die Ultrastruktur der Mitosekerne in den Plasmodien von Physarum polycephalum. Z. Zellforsch. 110, 108–130 (1970)

  28. Westergaard, M., Wettstein, D. von: The synaptinemal complex. Ann. Rev. Genet. 6, 71–110 (1972)

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Haskins, E.F. High voltage electron microscopical analysis of chromosomal number in the slime mold Echinostelium minutum de bary. Chromosoma 56, 95–100 (1976). https://doi.org/10.1007/BF00293109

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  • Electron Microscopy
  • Developmental Biology
  • Chromosomal Number
  • High Voltage
  • Microscopical Analysis