Chromosomes with Ag staining that varies from one metaphase to the other can be distinguished from those with an Ag-staining that is the same in all metaphases. The intercellular variation of an Ag-NOR can be attributed to many different factors. Whatever the importance of technical factors, they do not seem to account for the large variations in Ag-staining which were observed for each ac. This suggests the existence of a natural intercellular variability of the NOR's activity. The variation of the Ag-stainability of a given NOR, the diversity of Ag-stainings observed on the ten ac of one individual and the differences that exist between individuals raise the question of the existence of a compensation of activity between nucleolar organizers. The study, for each individual, of the mean sum of staining per metaphase reveals that this value is not absolutely constant from one individual to another; in the carriers of Robertsonian fusions it is smaller than in chromosomally normal individuals. The analysis of the transmission shows that inactive NORs remain inactive and that active NORs present a variation in the activity from one generation to the next.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Bloom SE, Goodpasture C (1976) An improved technique for selective silver-staining of nucleolar organizer regions in human chromosomes. Hum Genet 34:199–206
Dev VG, Byrne J, Bunch G (1979) Partial translocation of NOR and its activity in a balanced carrier and in her Cri-du-Chat fetus. Hum Genet 51:277–280
Howell WM, Denton TE, Diamond JR (1975) Differential staining of the satellite regions of human acrocentric chromosomes. Experientia 31:260–262
Howell WM, Black DA (1978) A rapid technique for producing silver-stained nucleolus organizer regions and trypsin-Giemsa bands on human chromosomes. Hum Genet 43:53–56
Jotterand M, van Melle G (1981) (In preparation)
Lau YF, Wertelecki W, Pfeiffer RA, Arrighi FE (1979) Cytological analyses of a 14p+ variant by means of N-banding and combinations of silver-staining and chromosome bandings. Hum Genet 46: 75–82
Markovic VD, Worton RG, Berg JM (1978) Evidence for the inheritance of silver-stained nucleolus organizer regions. Hum Genet 41:181–187
Mikelsaar AV, Schmid M, Krone W, Schwarzacher HG, Schnedl W (1977a) Frequency of Ag-stained nucleolus organizer regions in the acrocentric chromosomes of man. Hum Genet 37:73–77
Mikelsaar AV, Schwarzacher HG, Schnedl W, Wagenbichler P (1977b) Inheritance of Ag-stainability of nucleolar organizer regions. Hum Genet 38:183–188
Mikelsaar AV, Ilus T (1979) Populational polymorphism in silver-staining of nucleolus organizer regions (NORs) in human acrocentric chromosomes. Hum Genet 51:281–285
Miller DA, Dev VG, Tantravahi R, Miller OJ (1976) Suppression of human nucleolus organizer activity in mouse-human somatic hybrid cells. Exp Cell Res 101:235–243
Miller DA, Tantravahi R, Dev VG, Miller OJ (1977) Frequency of satellite association of human chromosomes is correlated with amount of Ag-staining of the nucleolus organizer region. Am J Hum Genet 29:490–502
Miller DA, Roy Berg W, Warburton D, Dev VG, Miller OJ (1978) Regulation of r-RNA gene expression in a human familial 14p+ marker chromosome. Hum Genet 43:289–297
Schwarzacher HG, Mikelsaar AV, Schnedl W (1978) The nature of the Ag-staining of nucleolus organizer regions. Cytogenet Cell Genet 20:24–39
Zankl H, Hahmann S (1978) Cytogenetic examination of the NOR activity in a proband with 13;13 translocation and in her relatives. Hum Genet 43:275–279
About this article
Cite this article
Jotterand-Bellomo, M., van Melle, G. Variability of the nucleolar organizer activity in human lymphocytes via Ag-staining. Hum Genet 59, 141–147 (1981). https://doi.org/10.1007/BF00293064
- Internal Medicine
- Metabolic Disease
- Normal Individual
- Human Lymphocyte
- Organizer Activity