Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Ultrastructure of cytoplasmic nucleolus-like bodies and nuclear RNP particles in late prophase of tipulid spermatocytes

Abstract

Late prophase stages of Pales ferruginea (Tipulidae) spermatocytes were examined by means of conventional electron microscopic section technique, combined with cytochemical methods. The cytoplasm of cells in diakinesis contains nucleolus-like bodies (NLB) 1 μm in diameter which are formed in diplotene at the pores of the nuclear membrane. They are compound structures consisting of fibro-granular RNP material which is associated with one or two electron-dense globules. The RNP material has a hollow core which contains polyribosomes. The NLBs possibly indicate rRNA gene amplification. At diakinesis the nucleus contains numerous electron-dense RNP particles scattered throughout the chromatin-free karyoplasm, and associated with the condensed chromosomes. The diameter of the chromatinassociated particles is markedly higher (mean 630 Å) than that of the free particles (mean 540 Å). The RNP particles seem to be aggregates of 200 Å subunits. They are regarded as transcription products of chromosomal genes.

This is a preview of subscription content, log in to check access.

References

  1. Bachellerie, J.-P., Puvion, E., Zalta, J.-P.: Ultrastructural organization and biochemical characterization of chromatin-RNA-protein complexes isolated from mammalian cell nuclei. Europ. J. Biochem. 58, 327–337 (1975)

  2. Bauer, H.: Die Chromosomen von Tipula paludosa Meig. in Eibildung und Spermatogenese. Z. Zellforsch. 14, 138–193 (1931)

  3. Bayreuther, K.: Die Oogenese der Tipuliden. Chromosoma (Berl.) 7, 508–557 (1956)

  4. Beermann, W.: Control of differentiation at the chromosomal level. J. exp. Zool. 157, 49–61 (1964)

  5. Bernhard, W.: A new staining procedure for electron microscopical cytology. J. Ultrastruct. Res. 27, 250–265 (1969)

  6. Brito da Cunha, A., Riess, R.W., Biesele, J.J., Morgante, I.S., Pavan, C., Garrido, M.C.: Studies on cytology and differentiation in Sciaridae. VI. Nuclear-cytoplasmic transfers in Hybosciara fragilis Morgante (Diptera, Sciaridae). Caryologia (Firenze) 26, 549–561 (1973)

  7. Callan, H.G., Lloyd, L.: Lampbrush chromosomes of crested newts, Triturus cristatus (Laurenti). Phil. Trans. Lond. B 243, 135–219 (1960)

  8. Chouinard, L.A.: An electron-microscope study of the extranuclear bodies during growth of the oocyte in the prepubertal mouse. J. Cell Sci. 12, 55–69 (1973)

  9. Fuge, H.: Spindelbau, Mikrotubuliverteilung und Chromosomenstruktur während der I. meiotischen Teilung der Spermatocyten von Pales ferruginea. Eine elektronenmikroskopische Analyse. Z. Zellforsch. 120, 579–599 (1971)

  10. Fuge, H.: Morphological studies on the structure of univalent sex chromosomes during anaphase movement in spermatocytes of the crane fly Pales ferruginea. Chromosoma (Berl.) 39, 403–417 (1972)

  11. Fuge, H.: Ultrastructure and function of the spindle apparatus. Microtubules and chromosomes during nuclear division. Protoplasma (Wien) 82, 289–320 (1974)

  12. Georgiev, G.P., Samarina, O.P.: D-RNA containing ribonucleoprotein particles. Advanc. Cell Biol. 2, 47–110 (1971)

  13. Halkka, L., Halkka, C.: Accumulation of gene products in the oocytes of the dragonfly Cordulia aenea L. I. The nematosomes. J. Cell Sci. 19, 103–115 (1975)

  14. Henderson, S.A.: RNA synthesis during male meiosis and spermatogenesis. Chromosoma (Berl.) 15, 345–366 (1964)

  15. Jaworska, H., Lima-de-Faria, A.: Amplification of ribosomal DNA in Achaeta. VI. Ultrastructure of two types of nucleolar components associated with ribosomal DNA. Hereditas (Lund) 74, 169–186 (1973a)

  16. Jaworska, H., Lima-de-Faria, A.: Amplification of ribosomal DNA in Achaeta. VII. Transfer of DNA-RNA-assemblies from the nucleus to the cytoplasm. Hereditas (Lund) 74, 187–204 (1973b)

  17. Kunz, W.: Lampenbürstenchromosomen und multiple Nukleolen bei Orthopteren. Chromosoma (Berl.) 21, 446–462 (1967)

  18. Kunz, W.: Die Entstehung multipler Oocytennukleolen aus akzessorischen DNS-Körpern bei Gryllus domesticus. Chromosoma (Berl.) 26, 41–75 (1969)

  19. La Cour, L.F., Wells, B.: The nucleolus at prophase of meiosis in three plants: an ultrastructural study. Proc. roy. Soc. B 191, 231–243 (1975)

  20. Lane, N.J.: Spheroidal and ring nucleoli in amphibian oocytes. J. Cell Biol. 35, 421–434 (1967)

  21. Lima-de-Faria, A.: Metabolic DNA in Tipula oleracea. Chromosoma (Berl.) 13, 47–59 (1962)

  22. Lima-de-Faria, A., Moses, M.J.: Ultrastructure and cytochemisty of metabolic DNA in Tipula. J. Cell Biol. 30, 177–192 (1966)

  23. Malcolm, D.B., Sommerville, J.: The structure of chromosome-derived ribonucleoprotein in oocytes of triturus cristatus carnifex (Laurenti). Chromosoma (Berl.) 48, 137–158 (1974)

  24. Miller, O.L., Beatty, B.R.: Nucleolar structure and functi on. In: Handbook of molecular cytology (A. Lima-de-Faria, ed.), pp. 605–618. Amsterdam 1969

  25. Monneron, A., Bernhard, W.: Fine structure organization of the interphase nucleus in some mammalian cells. J. Ultrastruct. Res. 27, 266–288 (1969)

  26. Mott, M.R., Callan, H.G.: An electron-microscope study of the lampbrush chromosomes of the newt Triturus cristatus. J. Cell Sci. 17, 241–261 (1975)

  27. Müller, W.: Elektronenmikroskopische Untersuchungen zum Formwechsel der Kinetochoren während der Spermatocytenteilungen von Pales ferruginea (Nematocera). Chromosoma (Berl.) 38, 139–172(1972)

  28. Nagl, W.: Origin and fate of the micronucleoli in the giant cells of the Phaseolus suspensor. Nucleus (Calcutta) 16, 100–109 (1973)

  29. Palombini, F., Stefanini, M.: Ultrastructural analysis of nucleolar evolution in the rat primary oocyte. J. Ultrastruct. Res. 47, 61–73 (1974)

  30. Perkowska, E., MacGregor, H.C., Birnstiel, M.L.: Gene amplification in the oocyte nucleus of mutant and wild-type Xenopus laevis. Nature (Lond.) 217, 649–659 (1968)

  31. Petzelt, C.: RNS- und Proteinsynthese im Ablauf der Spermatocytenteilungen von Pales ferruginea (Nematocera). Chromosoma (Berl.) 29, 237–245 (1970)

  32. Puvion, E., Bernhard, W.: Ribonucleoprotein components in liver cell nuclei as visualized by cryoultramicrotomy. J. Cell Biol. 67, 200–214 (1975)

  33. Reynolds, E.S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 209–213 (1963)

  34. Santolaya, R.C.: Nucleolus-like bodies in the neuronal cytoplasm of the mouse arcuate nucleus. Z. Zellforsch. 146, 319–328 (1973)

  35. Stevens, B.J.: Swift, H.: RNA transport from nucleus to cytoplasm in Chironomus salivery glands. J. Cell Biol. 31, 55–77 (1966)

  36. Vasquez-Nin, G., Bernhard, W.: Comparative ultrastructural study of perichromatin and Balbiani ring granules. J. Ultrastruct. Res. 36, 842–860 (1971)

  37. Williamson, R.: The protein moieties of animal messenger ribonucleoproteins. FEBS Letters (Amst.) 37, 1–6 (1973)

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Fuge, H. Ultrastructure of cytoplasmic nucleolus-like bodies and nuclear RNP particles in late prophase of tipulid spermatocytes. Chromosoma 56, 363–379 (1976). https://doi.org/10.1007/BF00292956

Download citation

Keywords

  • Gene Amplification
  • Nuclear Membrane
  • Free Particle
  • Chromosomal Gene
  • Condensed Chromosome