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Chromosomal proteins of Drosophila melanogaster and an approach for their localisation on polytene chromosomes

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Abstract

Chromosomal proteins have been prepared from embryos of Drosophila melanogaster and separated into histone and nonhistone fractions by a procedure which completely avoids exposure to extremes of pH. These fractions have been characterised by amino acid analysis and gel electrophoresis. Antisera have been prepared against whole chromatin and against the two chromosomal protein fractions. — A new method is described for the preparation of Drosophila salivary chromosomes. This method employs microdissection techniques and completely avoids the use of acid fixatives. Preservation of fine structure in these preparations is comparable to, if not better than, that in classical acid-fixed preparations. Antisera against embryo chromatin and chromosomal protein fractions react with the salivary chromosome preparations. These reactions exhibit selectivity with different chromosomal structures. Evidence is presented suggesting a specific distribution of protein antigens along the chromosome.

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Hill, R.J., Watt, F. Chromosomal proteins of Drosophila melanogaster and an approach for their localisation on polytene chromosomes. Chromosoma 63, 57–78 (1977). https://doi.org/10.1007/BF00292942

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Keywords

  • Developmental Biology
  • Fine Structure
  • Protein Fraction
  • Amino Acid Analysis
  • Protein Antigen