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Orientation of interphase chromosomes as detected by Giemsa C-bands

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The orientation of Giemsa C-bands has been studied in mitotic and interphase cells of Allium cepa, A. sativum and of Aloe vera. The C-bands in these three species are located at the telomeres, secondary constriction region of the nucleolar chromosomes and the centromeric regions, respectively. Observations in A. cepa and Aloe indicate clearly that the interphase chromosomes are non-random in their orientation and possibly maintain their telophase configuration through the attachment of telomeres and perhaps of kinetochores with the nuclear membrane. Electron micrographs of onion cells also reveal that certain heterochromatic segments are associated with the nuclear membrane. — The nucleolar interstitial C-bands in A. sativum remain free in the nucleoplasm and may come close to each other due to heterochromatic attraction. Such a heterochromatic attraction is also evident between telomeric regions and between centromeres. However, a two by two attachment could not be noticed. A diagrammatic representation of the orientation of interphase chromosomes has been presented.

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  1. Ashley, T., Wagenaar, E.B.: Telomeric associations of gametic and somatic chromosomes in diploid and autotetraploid Ornithogallum virens. Canad. J. Genet. Cytol. 19, 61–76 (1974)

  2. Bauer, H.: Structure and arrangement of salivary gland chromosomes in Drosophila species. Proc. nat. Acad. Sci. (Wash.) 22, 216–222 (1936)

  3. Church, K., Moens, P.B.: Centromere behavior during interphase and meiotic prophase in Allium fistulosum from 3-D, EM reconstruction. Chromosoma (Berl.) 56, 249–263 (1976)

  4. Comings, D.E.: The rationale for an ordered arrangement of chromatin in the interphase nucleus. Amer. J. hum. Genet. 20, 440–460 (1968)

  5. DuPraw, E.J.: The organization of nuclei and chromosomes in honeybee embryonic cells. Proc. nat. Acad. Sci. (Wash.) 53, 161–168 (1965)

  6. Eckhardt, R.A., Gall, J.G.: Satellite DNA associated with heterochromatin in Rhyncosciara. Chromosoma (Berl.) 32, 407–427 (1971)

  7. Fiskesjö: Chromosomal relationships between three species of Allium as revealed by C-banding. Hereditas (Lund) 81, 23–32 (1975)

  8. Fussel, C.P.: The position of interphase chromosomes and late replicating DNA in centromere and telomere regions of Allium cepa, L. Chromosoma (Berl.) 50, 201–210 (1975)

  9. Ghosh, S.: Nucleolar development in induced binucleate cells in Allium cepa. Cytobiologie 13, 163–167 (1976)

  10. Gill, B.S., Kimber, S.: The Giemsa C-banded karyotype of rye. Proc. nat. Acad. Sci. (Wash.) 71, 1247–1249 (1974)

  11. Heitz, E.: Die Herkunft der Chromocentren. Dritter Beitrag zur Kenntnis der Beziehung zwischen Kernstruktur und qualitativer Verschiedenheit der Chromosomen in ihrer Längsrichtung. Planta (Berl.) 34, 571–636 (1932)

  12. Hsu, T.C., Copper, J.E.K., Mace, M.L., Brinkley, B.R.: Arrangement of centromeres in mouse cells. Chromosoma (Berl.) 34, 73–87 (1971)

  13. Kitany, Y.: Orientation, arrangement and association of somatic chromosomes. Jap. J. Genet. 38, 244–256 (1963)

  14. Lafontaine, J.G.: A light and electron microscope study of small spherical nuclear bodies in meriste matic cells of Allium cepa, Vicia faba and Raphanus sativus. J. Cell Biol. 26, 1–17 (1965)

  15. Lafontaine, J.G.: Ultrastructural organization of plant cell nuclei. In: The cell nucleus (H. Busch, ed.), vol. 1, pp. 149–185. New York: Academic Press 1974

  16. Lafontaine, J.G., Lord, A.: Organization of nuclear structures in mitotic cells. In: Handbook of molecular cytology (A. Lima-de-Faria, ed.), pp. 381–411. Amsterdam: North Holland Publish ing Comp. 1969

  17. Natarajan, A.T., Ahnstrom, G.: Induced chromosomal abberations and heterochromatin. In: Modern aspects of cytogenetics-constitutive heterochromatin in man (R.A. Pfeiffer, ed.), pp. 201–213. Stuttgart-New York: Schattauer 1973

  18. Paweletz, N.: Elektronenmikroskopische Untersuchungen an frühen Stadien der Mitose bei HeLa Zellen. Cytobiologie 9, 368–390 (1974)

  19. Rabl, C.: Über Zellteilung. Morph. Jb. 10, 214–330 (1885)

  20. Schmid, M., Vogel, W., Krone, W.: Attraction between centric hetrochromatin of human chromosomes. Cytogenet. Cell Genet. 15, 66–80 (1975)

  21. Singh, R.J., Röbbelen, G., Okamoto, M.: Somatic association at interphase studied by Giemsa banding technique. Chromosoma (Berl.) 56, 265–273 (1976)

  22. Sutton, W.S.: On the morphology of group in Brachystola magna. Biol. Bull. 4, 24–39 (1902)

  23. Vanderlyn, L.: Somatic mitosis in roottip of Allium cepa. A review and orientation. Bot. Rev. 14, 270–318 (1948)

  24. Wagenaar, E.B.: End to end chromosome attachments in mitotic interphase and their possible significance to mitotic chromosome pairing. Chromosoma (Berl.) 26, 410–426 (1969)

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Ghosh, S., Roy, S.C. Orientation of interphase chromosomes as detected by Giemsa C-bands. Chromosoma 61, 49–55 (1977). https://doi.org/10.1007/BF00292679

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  • Developmental Biology
  • Electron Micrographs
  • Nuclear Membrane
  • Centromeric Region
  • Diagrammatic Representation