Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Decreased oxygen supply enhances growth in culture of human mid-trimester amniotic fluid cells

  • 30 Accesses

  • 14 Citations


Human mid-trimester amniotic fluid cells were cultivated under conditions of decreased oxygen supply. Compared to control cultures the low-oxygen group showed improved growth which was quantitated by three independent assays (1) direct cell counts, (2) bromodeoxyuridine (BrdU)-Hoechst flow-cytometry, and (3) cloning efficiency. The growth promoting effects of lowered oxygen hold for all major morphologic categories of amniotic fluid cells.

This is a preview of subscription content, log in to check access.


  1. Balin AK, Goodman DBP, Rasmussen H, Cristofalo VJ (1976) The effect of oxygen tension on the growth and metabolism of WI-38 cells. J Cell Physiol 89:235–250

  2. Chang H-C, Jones OW, Masui H (1982) Human amniotic fluid cells grown in a hormone-supplemented medium: Suitability for prenatal diagnosis. Proc Natl Acad Sci USA 79:4795–4799

  3. Cooper PD, Burt AM, Wilson JN (1958) Critical effect of oxygen tension on rate of growth of animal cells in continuous suspended culture. Nature 182:1508–1509

  4. Crickard K, Golbus MS (1982) Influence of extracellular matrix on the proliferation of human amniotic fluid cells in vitro. Prenat Diagn 2:89–96

  5. Cure S, Boué J (1973) A simple method for the determination of the growth rate in human fibroblast cell cultures. Experientia 29:207–209

  6. Dell'Aquila ML, Gaffney EV (1982) Growth or normal human amnion epithelial cells in serum free medium. Exp Cell Res 137: 441–446

  7. Eagle H (1973) The effect of environmental pH on the growth of normal and malignant cells. J Cell Physiol 82:1–8

  8. Epstein CJ (1982) The use of growth factors to stimulate the proliferation of amniotic fluid cells. Methods Cell Biol 26:269–276

  9. Gospodarowicz D, Moran JS, Owashi ND (1977) Effects of fibroblast growth factor and epidermal growth factor on the rate of growth of amniotic fluid-derived cells. J Clin Endocrinol Metab 44:651–659

  10. Hoehn H, Salk D (1982) Morphological and biochemical heterogeneity of amniotic fluid cells in culture. Methods Cell Biol 26:12–32

  11. Hoehn H, Rodriguez ML, Norwood TH, Maxwell CL (1978) Mosaicism in amniotic fluid cell cultures: Classification and significance. Am J Med Genet 2:253–266

  12. Joenje H, Arwert F, Erikson AW, de Koning H, Oostra AB (1981) Oxygen-dependence of chromosomal aberrations in Fanconi's anaemia. Nature 290:142–143

  13. Kubbies M, Rabinovitch PS (1983) Flow-cytometric analysis of factors which influence the BrdU-Hoechst quenching effect in human cultivated fibroblasts and lymphocytes. Cytometry 3: 276–281

  14. Packer L, Fuehr K (1977) Low oxygen concentration extends the lifespan of cultured human diploid cells. Nature 267:423–425

  15. Parshad R, Sanford KK (1971) Oxygen supply and stability of chromosomes in mouse embryo cells in vitro. J Natl Cancer Inst 47: 1033–1035

  16. Richter A, Sanford KK, Evans VJ (1972) Influence of oxygen and culture media on plating efficiency of some mammalian tissue cells. J Natl Cancer Inst 49:1705–1712

  17. Sanford KK, Parshad R, Jones G, Handleman S, Garrison C, Price F (1979) Role of photosensitization and oxygen in chromosome stability and “spontaneous” malignant transformation in culture. J Natl Cancer Inst 63:1245–1255

  18. Swartz HM (1973) Toxic oxygen effects. Int Rev Cytol 35:321–343

  19. Taylor WG, Camalier RF (1982) Modulation of epithelial cell proliferation in culture by dissolved oxygen. J Cell Physiol 111:21–27

  20. Taylor WG, Richter A, Evans VJ, Sanford KK (1974) Influence of oxygen and pH on plating efficiency and colony development of WI-38 and Vero cells. Exp Cell Res 86:152–156

  21. Taylor WG, Camalier RF, Sanford KK (1978) Density-dependent effects of oxygen on the growth of mammalian fibroblasts in culture. J Cell Physiol 95:33–40

  22. Werrlein RG, Glinos AD (1974) Oxygen microenvironment and respiratory oscillations in cultured mammalian cells. Nature 251:317–319

  23. Zwartouw HT, Westwood JCN (1958) Factors affecting growth and glycolysis in tissue culture. Br J Exp Pathol 39:529–539

Download references

Author information

Correspondence to M. Brackertz.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Brackertz, M., Kubbies, M., Feige, A. et al. Decreased oxygen supply enhances growth in culture of human mid-trimester amniotic fluid cells. Hum Genet 64, 334–338 (1983). https://doi.org/10.1007/BF00292364

Download citation


  • Oxygen
  • Internal Medicine
  • Cell Count
  • Metabolic Disease
  • Lowered Oxygen