Human mid-trimester amniotic fluid cells were cultivated under conditions of decreased oxygen supply. Compared to control cultures the low-oxygen group showed improved growth which was quantitated by three independent assays (1) direct cell counts, (2) bromodeoxyuridine (BrdU)-Hoechst flow-cytometry, and (3) cloning efficiency. The growth promoting effects of lowered oxygen hold for all major morphologic categories of amniotic fluid cells.
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Brackertz, M., Kubbies, M., Feige, A. et al. Decreased oxygen supply enhances growth in culture of human mid-trimester amniotic fluid cells. Hum Genet 64, 334–338 (1983). https://doi.org/10.1007/BF00292364
- Internal Medicine
- Cell Count
- Metabolic Disease
- Lowered Oxygen