Advertisement

Springer Nature is making Coronavirus research free. View research | View latest news | Sign up for updates

Mapping parathyroid hormone, β-globin, insulin, and LDH-A genes within the human chromosome 11 short arm by spot blotting sorted chromosomes

Summary

Rearranged human chromosomes carrying segments of chromosome 11 were separated from the normal chromosome 11 by high-resolution chromosome sorting. Sorted chromosomes were tested with parathyroid hormone, β-globin, insulin, and LDH-A gene-specific probes to determine the genes carried by each chromosome segment. Based on the gene content and karyotypes of these abnormal chromosomes, the parathyroid hormone, β-globin, insulin, and LDH-A genes and the unique restriction fragment ADJ-762 are all located on the terminal band of the short arm of human chromosome 11 (band 11p15), with LDH-A proximal to the other loci.

This is a preview of subscription content, log in to check access.

References

  1. Aronson MM (1983) Human genetic mutant cell repository catalog, Institute for Medical Research. Copewood and Davis Sts, Camden

  2. Barker D, Holm T, Jorde L, White R (to be published) Locus on chromosome 11p with multiple restriction site polymorphisms

  3. Bruns G, manuscript in preparation

  4. Deisseroth A, Nienhuis A, Lawrence J, Giles R, Turner P, Ruddle FH (1978) Chromosomal localization of human β-globin gene on human chromosome 11 in somatic cell hybrids. Proc Natl Acad Sci USA 75:1456–1460

  5. de Martinville B, Francke U (1983) Hras-1, insulin and β-globin loci map outside the deletion associated with aniridia-Wilms' tumor. Nature 305:641–643

  6. Donlon TA, Harper ME, Magenis RE (1984) Use of chromosome rearrangements to more precisely localize the insulin gene with in situ hybridization. Cytogenet Cell Genet 37:455

  7. Fearon E, Vogelstein B, Feinberg AP (1984) Somatic deletion and duplication of genes on chromosome 11 in Wilms' tumor. Nature 309:176–178

  8. Ferrell RE, Riccardi VM (1981) Catalase levels in patients with aniridia and/or Wilms' tumor: utility and limitations. Cytogenet Cell Genet 31:120–123

  9. Francke U, George DL, Brown MG, Riccardi VM (1977) Gene dose effect: intraband mapping of the LDH-A locus using cells from four individuals with different interstitial deletions of 11p. Cytogenet Cell Genet 19:197–207

  10. Gerhard D, Kidd K, Kidd J, Gusella J, Housman D (1984) Genetic linkage studies of human chromosome 11. In: Advances in gene technology: human genetic disorders, Proc. 16th Miami Winter Symp, pp 172–173

  11. Gusella J, Varsanyi-Breiner A, Kao F-T, Jones C, Puck TT, Keys C, Orkin S, Houseman D (1979) Precise localization of human β-globin gene complex on chromosome 11. Proc Natl Acad Sci USA 76:5239–5243

  12. Harper ME, Ullrich A, Sainders GF (1981) Localization of the human insulin gene to the distal end of the short arm of chromosome 11. Proc Natl Acad Sci USA 78:4458–4460

  13. Huerre C, Gilgenkrantz S, Leonard C, Pictet R, Kaplan JC, Junien C (1984) Regional assignment of the structural gene for insulin to 11p15.1→11p15.5 by deletion mapping. Cytogenet Cell Genet 37:495

  14. ISCN (1981) An international system for human cytogenetic nomenclature—high resolution banding. Cytogenet Cell Genet 31:1–23 (1981) and National Foundation-March of Dimes (1972) Birth Defects VIII:vol 7

  15. Jhanwar SC, Neel BG, Hayward WS, Chaganti RSK (1983) Localization of c-ras oncogene family on human germ-line chromosomes. Proc Natl Acad Sci USA 80:4794–4797

  16. Lebo RV (1982) Chromosome sorting and DNA sequence localization. Cytometry 3:145–154

  17. Lebo RV, Bastian AM (1982) Design and operation of a dual laser chromosome sorter. Cytometry 3:213–219

  18. Lebo RV, Carrano AV, Burkhart-Schultz KJ, Dozy AM, Yu L-C, Kan YW (1979) Assignment of human β-, γ- and δ-globin genes to the short arm of chromosome 11 by chromosome sorting and DNA restriction enzyme analysis. Proc Natl Acad Sci USA 76:5804–5808

  19. Lebo RV, Kan YW, Cheung M-C, Carrano AV, Yu L-C, Chang JC, Cordell B, Goodman HM (1982) Assigning the polymorphic human insulin gene to the short arm of chromosome 11 by chromosome sorting. Hum Genet 60:10–16

  20. Lebo RV, Gorin F, Fletterick RJ, Kao F-T, Cheung M-C, Bruce BD, Kao F-T, Kan YW (1984) High-resolution chromosome sorting and DNA spot-blot analysis localize McArdle's syndrome to chromosome 11. Science 225:57–59

  21. Leif RC, Easter HN, Waters RL, Thomas RA, Dunlap LA, Austin MF (1971) A quantitative technique for the preparation of glutaraldehyde-fixed cells for the light and scanning electron microscope. J Histochem Cytochem 19:203–215

  22. Malcolm S, Barton P, Murphy C, Ferguson-Smith MA (1981) Chromosomal localization of a single copy gene by in situ hybridization-human β-globin genes on the short arm of chromosome 11. Ann Hum Genet 45:135–141

  23. Mendelsohn ML, Mayall BH, Bogart E, Moore DH, Perry BH (1973) DNA content and DNA-based centromeric index of the 24 human chromosomes. Science 179:1126–1129

  24. Meyers DA, Antonarakis SE, Fearon ER, Mallonee RL, Waber PG, Phillips JA III, Kazazian HH Jr, Levine M, Kronenberg HM, Wigler M, Ullrich A (1984) LUKse of DNA polymorphisms to establish the linkage map of the PTH, beta-globin, c-Ha-ras-1 and insulin loci on 11p. Cytogenet Cell Genet 37:540

  25. Morton CC, Kirsch IR, Taub R, Orkin SH, Brown JA (1984) Localization of the β-globin gene by chromosomal in situ hybridization. Am J Hum Genet 36:576–585

  26. Naylor SL, Sakaguchi AY, Szoka P, Hendy GN, Kronenbert HM, Rick A, Shows TB (1984) Parathyroid hormone gene is on the short arm of human chromosome 11. Cytogenet Cell Genet 37:551

  27. Owerbach D, Bell GI, Rutter WJ, Brown JA, Shows TB (1981) The insulin gene is located on the short arm of chromosome 11 in humans. Diabetes 30:267–270

  28. Riccardi VM, Sujansky E, Smith AC, Francke U (1978) Chromosomal imbalance in the aniridia-Wilms' tumor association: 11p interstitial deletion. Pediatrics 61:604–610

  29. Rigby PWJ, Dieckmann M, Rhodes C, Berg P (1977) Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol 113:237–251

  30. Scott AF, Phillips JA III, Migeon BR (1979) DNA restriction endonuclease analysis for localization of human β- and δ-globin genes on chromosome 11. Proc Natl Acad Sci USA 76:4563–4565

  31. Solomon E (1984) Recessive mutation in aetiology of Wilms' tumor. Nature 309:111–112

  32. White R, Leppert M, Bishop DT, Barker D, Berkowitz J, Brown C, Callahan P, Holm T, Jerominski LA (1984) Linkage map for the human based on DNA markers: chromosome 11p. In: Ulrich A, Wolf S (eds) Chromosomes today, vol 8. George Allen, London

  33. Young BD, Ferguson-Smith MA, Sillar R, Boyd E (1982) High resolution analysis of human peripheral lymphocyte chromosomes by flow cytometry. Proc Natl Acad Sci USA 78:7727–7731

Download references

Author information

Correspondence to Roger V. Lebo.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Lebo, R.V., Cheung, M., Bruce, B.D. et al. Mapping parathyroid hormone, β-globin, insulin, and LDH-A genes within the human chromosome 11 short arm by spot blotting sorted chromosomes. Hum Genet 69, 316–320 (1985). https://doi.org/10.1007/BF00291648

Download citation

Keywords

  • Internal Medicine
  • Metabolic Disease
  • Restriction Fragment
  • Parathyroid Hormone
  • Human Chromosome