Archives of Microbiology

, Volume 156, Issue 2, pp 148–151 | Cite as

Mono-association of mice with non-cultivable, intestinal, segmented, filamentous bacteria

  • H. L. B. M. Klaasen
  • J. P. Koopman
  • M. E. Van den Brink
  • H. P. N. Van Wezel
  • A. C. Beynen
Original Papers


A technique is described so that mice mono-associated with non-cultivable, segmented filamentous bacteria (SFB's) can be produced for the first time. As SFB donors, mice were used which had an intestinal microflora consisting of both SFB's and bacteria of the genus Clostridium. Recipients were germ-free mice. It was demonstrated that the intraileal inoculation method was more effective than the orogastric route. Therefore, intestinal homogenates of donor mice were treated with filtered ethanol, diluted and administered intraileally to recipient mice. Evidence is presented that cage mates of the recipient mice were mono-associated with SFB's. The availability of these animals, i.e. in vivo monocultures of SFB's, allows taxonomic and functional characterization of SFB's, which was as yet not possible.

Key words

Germ-free mice Intestinal bacteria Mouse Segmented Filamentous bacteria 



Segmented filamentous bacterium


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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • H. L. B. M. Klaasen
    • 1
  • J. P. Koopman
    • 1
  • M. E. Van den Brink
    • 1
  • H. P. N. Van Wezel
    • 1
  • A. C. Beynen
    • 2
    • 3
  1. 1.Central Animal LaboratoryCatholic UniversityNijmegenThe Netherlands
  2. 2.Department of Laboratory Animal ScienceState UniversityUtrechtThe Netherlands
  3. 3.Department of Human NutritionAgricultural UniversityWageningenThe Netherlands

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