Archives of Microbiology

, Volume 156, Issue 2, pp 142–144 | Cite as

Artificial DNA-mediated genetic transformation of the photosynthetic nitrogen-fixing bacterium Rhodospirillum rubrum

  • Wayne P. Fitzmaurice
  • Gary P. Roberts
Original Papers


Methods were identified for the introduction of plasmid DNA into Rhodospirillum rubrum, including freeze-thaw and CaCl2-based techniques.

Key words

Genetic transformation pSa4 Rhodospirillum rubrum 



colony forming units


dimethyl sulfoxide




optical density at 680 nm


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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Wayne P. Fitzmaurice
    • 1
    • 2
  • Gary P. Roberts
    • 1
    • 2
  1. 1.Department of BacteriologyUniversity of Wisconsin-MadisonMadisonUSA
  2. 2.the Center for the Study of Nitrogen FixationUniversity of Wisconsin-MadisonMadisonUSA

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