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Archives of Microbiology

, Volume 156, Issue 2, pp 111–114 | Cite as

An electron microscopy study of wall expansion during Candida albicans yeast and mycelial growth using concanavalin A-ferritin labelling of mannoproteins

  • Hortensia Rico
  • Enrique Herrero
  • Fernando Miragall
  • Rafael Sentandreu
Original Papers

Abstract

Depending upon growth temperature, Candida albicans can exhibit two different morphologies, a budding yeast or a mycelium. By studying the distribution of concanavalin A-ferritin particles on the cell wall surface during bud and germ tube formation, we have elucidated the way cell wall extension occurs. Both processes initially require the localized lysis of the wall in order to allow the incorporation of the newly synthesized material. Later on, the cell wall behaves as an elastic structure, allowing extension by an intosusception process and, as a consequence, cell growth.

Key words

Candida albicans Cell growth Budding Mycelium Cell wall Mannoproteins Concanavalin A 

Abbreviation

Con A

concanavalin A

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References

  1. Bartnicki-García S (1973) Fundamental aspects of hyphal morphogenesis. Symp Soc Gen Microbiol 23: 245–267Google Scholar
  2. Biely P, Kovarik J, Bauer S (1973) Cell wall formation in yeast, an electron microscopic autoradiographic study. Arch Microbiol 94: 365–371Google Scholar
  3. Buffo J, Herman MA, Soll DR (1984) A characterization of pH-regulated dimorphism in Candida albicans. Mycopathologia 85: 21–30CrossRefGoogle Scholar
  4. Elorza MV, Rico H, Sentandreu R (1983) Calcofluor white alters the assembly of chitin fibrils in Saccharomyces cerevisiae and Candida albicans cells. J Gen Microbiol 129: 1577–1582PubMedGoogle Scholar
  5. Elorza MV, Murgui A, Sentandreu R (1985) Dimorphism in Candida albicans: contribution of mannoproteins to the architecture of yeast and mycelial cell walls. J Gen Microbiol 131: 2209–2216PubMedGoogle Scholar
  6. Farkas V, Kovarik J, Kosinova A, Bauer S (1974) Autoradiographic study of mannan incorporation into growing cells walls of Saccharomyces cerevisiae. J Bacteriol 117: 265–269PubMedPubMedCentralGoogle Scholar
  7. Goldstein IJ, Poretz RD (1986) Isolation, physicochemical characterization and carbohydrate-binding specificity of lectins. In: Liener E, Sharon N, Goldstein IJ (eds) The lectins: properties, functions, and applications in biology and medicine. Academic Press, Orlando, pp 33–247CrossRefGoogle Scholar
  8. Hartwell LH, Unger MW (1977) Unequal division in Saccharomyces cerevisiae and its implications for the control of cell division. J Cell Biol 75: 422–435CrossRefGoogle Scholar
  9. Herman MA, Soll DR (1984) A comparison of volume growth during bud and mycelium formation in Candida albicans: a single cell analysis. J Gen Microbiol 130: 2219–2228PubMedGoogle Scholar
  10. Johnston GC, Pringle JR, Hartwell LH (1977) Coordination of growth with cell division in the yeast Saccharomyces cerevisiae. Exp Cell Res 105: 79–98CrossRefGoogle Scholar
  11. Lee KL, Buckley HR, Campbell C (1975) An aminoacid liquid synthetic medium for development of mycelial and yeast forms of Candida albicans. Sabouraudia 13: 148–153CrossRefGoogle Scholar
  12. Mattia E, Carruba G, Angiolella L, Cassone A (1982) Induction of germ-tube formation by N-acetyl-d-glucosamine in Candida albicans: uptake of inducer and germinative response. J Bacteriol 152:55–562Google Scholar
  13. Miragall F, Rico H, Sentandreu R (1988) Regeneration of the cell wall in protoplasts of Candida albicans. A cytochemical study using wheat germ agglutimin and Concanavalin A. Arch Microbiol 149:286–290CrossRefGoogle Scholar
  14. Mitchell L, Soll DR (1979) Commitment to germ tube or bud formation during release from stationary phase in Candida albicans. Exp Cell Res 120: 167–179CrossRefGoogle Scholar
  15. Odds FC (1988) Candida and candidosis. Bailliere-Tindall, LondonGoogle Scholar
  16. Pringle JR, Hartwell LH (1981) The Saccharomyces cerevisiae cell cycle. In: Strathern JN, Jones EW, Broach JR (eds) The molecular biology of the yeast Saccharomyces, vol 1. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 97–142Google Scholar
  17. Shepherd MG, Poulter RTM, Sullivan PA (1985) Candida albicans: biology, genetics and pathogenicity. Ann Rev Microbiol 39:579–614CrossRefGoogle Scholar
  18. Soll DR, Mitchell LH (1983) Filament ring formation in the dimorphic yeast Candida albicans. J Cell Biol 96:486–493CrossRefGoogle Scholar
  19. Spurr AR (1969) A low viscosity epoxy resin embedding medium for electron microscopy. J Ultrastr Res 26:31–43CrossRefGoogle Scholar
  20. Staebell M, Soll DR (1985) Temporal and spatial differences in cell wall expansion during bud and mycelium formation in Candida albicans. J Gen Microbiol 131:1467–1480PubMedGoogle Scholar
  21. Sudbery PE, Goodey AR, Carter BLA (1980) Genes which control cell proliferation in the yeast Saccharomyces cerevisiae. Nature (Lond) 288:401–404CrossRefGoogle Scholar
  22. Tkacz JS, Lampen JO (1972) Wall replicas in Saccharomyces cerevisiae species: use of fluorescein conjugated Concanavalin A to reveal the site of mannan insertion. J Gen Microbiol 72:243–247CrossRefGoogle Scholar
  23. Tronchin G, Poulain D, Diguet J (1979) Etudes cytochimiques et ultrastructurales de la paroi de Candida albicans. I. Localization des mannanes par utilisation de Concanavalin A sur coupes ultrafines. Arch Microbiol 123:245–249CrossRefGoogle Scholar
  24. Tronchin G, Poulain D, Herbaut J, Biguet J (1981) Cytochemical and ultrastructural studies of Candida albicans. II. Evidence for cell wall coat using Concanavalin A. J Ultrastr Res 75: 50–59CrossRefGoogle Scholar
  25. Wheals AE (1987) Biology of the cell cycle in yeast. In: Rose AH, Harrison JS (eds) The yeasts, vol 1. Academic Press, London, pp 283–390Google Scholar
  26. Wessels JGH, Mol PC, Sietsma JH, Vermeulen CA (1990) Wall structure, wall growth, and fungal cell morphogenesis. In: Kuhn PJ, Trinci APJ, Jung MJ, Goosey MW, Copping LG (eds) Biochemistry and cell walls and membranes in fungi. Springer, Berlin Heidelberg New York, pp 81–95CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Hortensia Rico
    • 1
  • Enrique Herrero
    • 1
  • Fernando Miragall
    • 1
  • Rafael Sentandreu
    • 1
  1. 1.Departament de Microbiología, Facultat de FarmàciaUniversidat de ValènciaValènciaSpain

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