The DNA:histone ratios have been determined by quantitative cytochemical analyses of individual cells in populations of human lymphocytic cells derived in continuous culture from the peripheral blood buffy coats of patients with acute leukemia or infectious mononucleosis. These populations of lymphocytic cells were quite similar with respect to the Feulgen-DNA and protein content per cell. The close association between DNA and histone was reflected in their similar patterns of distribution in fixed and stained cells; and further evidenced by similarities in the DNA: histone ratios characteristic of these different populations of lymphocytic cells. — Chemical acetylation and methylation of nuclear proteins of these cell populations exhibited some quantitative differences. The chemically acetylated histone content was less, and chemically methylated histone content was greater in cells derived from acute leukemia or infectious mononucleosis than in normal human lymphocytes. These quantitative differences in chemical acetylation and methylation may contribute to specific structural alterations in these histones which modify their functional capacity with respect to interactions with DNA. Such alterations may relate to differences in gene expression as reflected, for example, by the biological and biochemical differences among these human lymphocytic cells.
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Adams, R. A., Flowers, A., Davis, B. J.: Direct implantation and serial transplantation of human acute lymphoblastic leukemia in hamsters, SB-2. Cancer Res. 28, 1121–1125 (1968).
Adams, R. A., Foley, G. E., Pothier, L., Lazarus, H., Stuart, A.: Infectious Mononucleosis: Serial heterotransplantation of a cell line isolated from peripheral blood. Proo. Amer. Ass. Cancer Res. 10, 2 (1969).
Alfert, M., Geschwind, I.: A selective staining method for the basic proteins of cell nuclei. Proc. Nat. Acad. Sci. (Wash.) 39, 991–999 (1953).
Allfrey, V. G., Pogo, B. G. T., Littau, V. C., Gershey, E. L., Mirsky, A. E.: Histone acetylation in insect chromosomes. Science 159, 314–316 (1968).
Alvarez, M. R.: Cytophotometric study of nuclear proteins and nucleic acids in parenchymatous tissue of the orchid embryo. Exp. Cell Res. 57, 179–184 (1969).
Bonner, J., Huang, R. C.: Role of histone in chromosomal RNA synthesis. In: The nucleohistones (J. Bonner and P. Ts'o, eds.), p. 251–261. San Francisco: Holden-Day, Inc. 1964.
Borek, E., Srinivasan, P. R.: The methylation of nucleic acids. Ann. Rev. Biochem. 35, 275–298 (1966).
Caspersson, T. O., Farber, S., Foley, G. E., Lomakka, G., Killander, D., Carlson, L.: Cytochemical study of nucleolus-ribosome system in populations of normal and neoplastic cells. Exp. Cell Res. 28, 621–623 (1962).
Caspersson, T., Foley, G. E., Killander, D., Lomakka, G.: Cytochemical differences between mammalian cell lines of normal and neoplastic origins: Correlation with heterotransplantability in Syrian hamsters. Exp. Cell Res. 32, 553–565 (1963).
Deitch, A. D.: Cytophotometry of proteins. In: Introduction to quantitative cytochemistry (G. L. Weid, ed.) p. 451–468. New York: Academic Press, Inc. 1966.
Desai, L. S., Tencer, R.: Effect of histone and polylysine on the synthetic activity of the giant chromosomes of salivary glands of Dipteran larvae. Exp. Cell Res. 52, 185–197 (1968).
Desai, L. S., Foley, G. E.: Homologies in the amino acid composition and structure of histone F2al isolated from human leukaemic cells. Biochem. J. 119, 165–170 (1970a).
Desai, L. S., Foley, G. E.: Studies of the nucleic acids of human lymphocytic cells: Acetylation of histones. Arch. Biochem. Biophys. 141, 552–556 (1970b).
Desai, L. S., Foley, G. E.: Human leukemic cells: Inhibitory effects of isologous and homologous histones. Exp. Cell Res. 66, 1–4 (1971).
Eagle, H.: Amino acid metabolism in mammalian cell cultures. Science 130, 432–437 (1959).
Earle, W. R., Schilling, E. L., Stark, T. H., Straus, N. P., Brown, M. F., Shelton, E. Production of malignancy in vitro; mouse fibroblast cultures and changes seen in living cells. J. nat. Cancer Inst. 4, 165–212 (1943).
Foley, G. E., Lazarus, H., Farber, S., Uzman, B. G., Adams, R. A.: Studies on human leukemic cells in vitro. In: The proliferation and spread of neoplastic cells. 21st Ann. Symp. on Fundamental Cancer Res., University of Texas M. D. Anderson Hospital and Tumor Institute, Houston, Texas (1967), p. 65–97. Baltimore, Maryland: The Williams and Wilkins Co. 1968.
Foley, G. E., Lazarus, H., Farber, S., Uzman, B. G., Boone, B. A., MacCarthy, R. A.: Continuous culture of human lymphoblasts from peripheral blood of a child with acute leukemia. Cancer (Philad.) 18, 522–529 (1965).
Frenster, J. H.: Localized strand separations with deoxyribonucleic acid during selective transcription. Nature (Lond.) 208, 894–896 (1965a).
Frenster, J. H.: Correlation of the binding to DNA loops or to DNA helices with the effect on RNA synthesis. Nature (Lond.) 208, 1093 (1965b).
Gahrton, G., Foley, G. E.: Leukemia-like pattern of DNA, RNA and protein content of individual mononuclear cells in the peripheral blood of patients with infectious mononucleosis. Cancer Res. 29, 1076–1081 (1969).
Gershey, E. L., Vidali, G., Allfrey, V. G.: Chemical studies of histone aoetylation. The occurrence of ɛ-N-acetyllysine in the f2al histone. J. biol. Chem. 243, 5018–5022 (1968).
Gorovsky, M. A., Woodard, J.: Histone content of chromosomal loci active and inactive in RNA synthesis. J. Cell Biol. 33, 723–728 (1967).
Leuchtenberger, C.: Quantitative determination of DNA in cells by Feulgen microspectrophotometer. In: General cytochemical methods, vol. 1 (J. F. Danielli ed.), p. 219–278. New York: Academic Press, Inc. 1968.
Lomakka, G.: A rapid scanning and integrating cytophotometer. Acta histochem. (Jena), Suppl, 6, 47–54 (1965a).
Lomakka, G.: A rapid scanning and integrating microinterferometer. Acta histochem. (Jena), Suppl. 6, 393–396 (1965b).
McCarthy, R. E., Gahrton, G., Farber, S., Foley, G. E.: Cytochemical population analyses of continuous cultures of human lymphoblasts derived from acute lymphoblastic leukemia. Exp. Cell Res. 43, 564–570 (1966).
Swift, H.: The histones of polytene chromosomes. In: The nucleohistones (J. Bonner and P. Ts'o, eds.), p. 169–183. San Francisco: Holden-Day, Inc. 1964.
Terner, J. Y.: Histochemical alkylation: A study of methyliodide and its effect on tissues. J. Histochem. Cytochem. 12, 504–511 (1964).
Vidali, G., Gershey, E. L., Allfrey, V. G.: Chemical studies of histone acetylation. The distribution of ɛ-N-acetyllysine in calf thymus histones. J. biol. Chem. 243, 6361–6366 (1968).
These studies were supported in part by research grants C-6516 from the National Cancer Institute and FR-05526 from the Division of Research Facilities and Resources, National Institutes of Health.
Holds Research Career Award K6-CA-22,150 from the National Cancer Institute, National Institutes of Health.
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Desai, L.S., De Bault, L.E., Morrissey, G. et al. Human leukemic cells: Cytochemical studies on nuclear proteins. Chromosoma 38, 329–340 (1972). https://doi.org/10.1007/BF00290930
- Nuclear Protein
- Continuous Culture
- Acute Leukemia
- Quantitative Difference
- Human Lymphocyte