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Heat shock protein HSP60 can alleviate the phenotype of mitochondrial RNA-deficient temperature-sensitive mna2 pet mutants

Abstract

mna2, which belongs to the class I temperature-sensitive pet mutants that lose mitochondrial (mt)RNA at restrictive temperature, was shown by complementation and sequence determination to correspond to the gene coding for HSP60. Both mna2-1 and mna2-2, the two available alleles of mna2, have conservative single amino acid substitutions in the HSP60 gene. Valine substitutes for an alanine (position 47) in mna2-1, and an isoleucine substitutes for a valine (position 77) in mna2-2. These substitutions result in defects in respiration and in steady-state mtRNA accumulation. Wild-type hsp60 alleviates the mtRNA phenotype completely, while partially relieving the respiratory deficiency.

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Author information

Correspondence to Arunik Sanyal.

Additional information

Communicated by D. M. Lonsdale

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Sanyal, A., Harington, A., Herbert, C.J. et al. Heat shock protein HSP60 can alleviate the phenotype of mitochondrial RNA-deficient temperature-sensitive mna2 pet mutants. Molec. Gen. Genet. 246, 56–64 (1995). https://doi.org/10.1007/BF00290133

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Key words

  • Temperature-sensitive mutants
  • Heat shock protein 60
  • Conservative single amino acid substitutions
  • Mitochondrial biogenesis
  • Saccharomyces cerevisiae