Chromosome analyses were carried out in lymphocytes of ten children with ALL, prior to and during combined therapy with antineoplastic drugs and cranial irradiation. Chromosome preparations from group I (four patients) were analysed by conventional staining. For group II (six patients) the FPG technique (fluorescence plus Giemsa) was applied after BrdU treatment of cultures and cells were scored exclusively in first division. A significant clastogenic effect could not be detected in samples collected during or after chemotherapy for either group. After cranial irradiation of those patients in group II, linear dose-effect relationships for dicentric plus ring chromosomes, and for excess acentrics, could be demonstrated after correction for the relative amount of irradiated volume.
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Apelt F, Kolin-Gerresheim I, Bauchinger M (1981) Azathioprine, a clastogen in human somatic cells? Analysis of chromosome damage and SCE in lymphocytes after exposure in vivo and in vitro. Mutat Res 88:61–72
Bauchinger M (1983) Cytogenetic effects in human lymphocytes as a dosimetry system. In: Eisert WG, Mendelsohn ML (eds) Proceedings of “Biological dosimetry: cytometric approaches to mammalian systems”. Neuherberg, 14–16 October 1982. Springer, Berlin Heidelberg New York, in press
Bauchinger M, Schmid E (1969) Cytogenetische Veränderungen in weißen Blutzellen nach Cyclophosphamidtherapy. Z Krebsforsch 72:77–87
Bauchinger M, Kühn H, Dresp J, Schmid E, Streng S (1983) Dose effect relations for 14.5 (d+T) neutron induced chromosome aberrations in human lymphocytes irradiated in a man phantom. Int J Radiat Biol, in press
Bell WR, Whang JJ, Carbone PP, Brecher G, Block JB (1966) Cytogenetic and morphologic abnormalities in human bone marrow cells during cytosine arabinoside therapy. Blood 27:771–781
Fischer P, Nacheva E, Pohl-Rüling J, Kepler P (1978) Cytogenetic effects of chemotherapy and cranial irradiation on the peripheral blood lymphocytes of children with leukaemia. In: Evans HJ, Lloyd DC (eds) Mutagen-induced chromosome damage in man. University Press, Edinburgh
Gebhart E, Schwanitz G, Hartwich G (1969) Zytogenetische Wirkung von Vincristin auf menschliche Leukozyten in vivo und in vitro. Med Klin 64:2366–2371
Gebhart E, Lösing J, Wopfner F (1980) Chromosome studies on lymphocytes of patients under cytostatic therapy. I. Conventional chromosome studies in cytostatic interval therapy. Hum Genet 55: 53–63
Hampel KE, Kober B, Rösch P, Gerhartz H, Meining KH (1966) The action of cytostatic agents on the chromosome of human leucocytes in vitro. Blood 27:816–823
Jensen MK, Nyfors A (1979) Cytogenetic effects of methotrexate on human cells in vivo. Mutat Res 64:339–343
Kellerer AM, Brenot J (1974) On the statistical evaluation of doseresponse function. Radiat Environ Biophys 11:1–13
Kolin-Gerresheim I, Bauchinger M (1981) Dependence of the frequency of Harlequin-stained cells on BrdU concentration in human lymphocyte cultures. Mutat Res 91:251–254
Lloyd DC (1983) Overview of biological dosimetry of chromosomes and its application using conventional methods. In: Eisert WG, Mendelsohn ML (eds) Proceedings of “Biological dosimetry: cytometric approaches to mammalian systems”. Neuherberg, 14–16 October 1982. Springer, Berlin Heidelberg New York, in press
Matsubara S, Sasaki MS, Adachi T (1974) Dose response relationship of lymphocyte chromosome aberrations in locally irradiated persons. J Radiat Res 15:189–196
Miller PC, Hill RB, Nichols WW, Meadows AT (1978) Acute and longterm cytogenetic effects of childhood cancer chemotherapy and radiotherapy. Cancer Res 38:3241–3246
Nevstad NP (1977) Sister chromatid exchanges and chromosomal aberrations induced in human lymphocytes by the cytostatic drug adriamycin in vivo and in vitro. Mutat Res 57:253–258
Perry P, Wolff S (1974) New giemsa method for the differential staining of sister chromatids. Nature 252:156–158
Ryan TJ, Boddington MM, Springs AJ (1965) Folic acid antagonists and chromosomal abnormalities. Br J Dermatol 77:283
Sachsse W, Denk R (1970) Chromosomenuntersuchungen aus Lymphocyten und Knochenmark von Psoriasis-Kranken nach Langzeitbehandlung mit Methotrexat. Arch Klin Exp Derm 239:275–281
Saller K (1964) Leitfaden der Anthropologie. Gustav Fischer, Stuttgart, S 311–312
Schmid E, Bauchinger M (1973) Comparison of the chromosome damage induced by radiation and cytoxan therapy in lymphocytes of patients with gynaecological tumours. Mutat Res 21:271–274
Vorhees JJ, Janzen MK, Harrell ER, Chakrabarti SG, Arbor A (1969) Cytogenetic evaluation of methotrexate-treated psoriatic patients. Arch Dermatol 100:268–274
Vormittag W, Pietschmann H (1972) Untersuchungen von Lymphozyten nach zytostatischer Therapie mit l-Asparaginase. Wiener Klin Wochenschr 84:64–67
Wagner R, Schmid E, Bauchinger M (1983) The application of conventional and FPG-staining for the analysis of chromosome aberrations induced by low levels of dose in human lymphocytes. Mutat Res, 109:65–71
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Rausher, K.H., Bauchinger, M. Chromosome aberrations induced in patients treated with chemotherapeutic drugs and irradiation for acute lymphatic leukemia. Hum Genet 64, 73–79 (1983). https://doi.org/10.1007/BF00289483
- Internal Medicine
- Metabolic Disease
- Combine Therapy
- Chemotherapeutic Drug