Theoretical and Applied Genetics

, Volume 73, Issue 2, pp 272–277 | Cite as

Peptidases A, B, C, D and S in the American mink: polymorphism and chromosome localization

  • M. R. Mullakandov
  • A. A. Gradov
  • S. M. Zakijan
  • N. B. Rubtsov
  • O. L. Serov


An electrophoretic analysis of peptidases was carried out in a population of American mink. Based on substrate and tissue specificities, as well as subunit composition, homologies were established between mink peptidases A, B, C, D and S and human peptidases. Polymorphism for peptidases B and D was demonstrated for minks of three coat colour types. Breeding data indicated that the peptidase variations are under the control of allele pairs at distinct autosomal loci designated as PEPB and PEPD, respectively. Using a panel of American mink-Chinese hamster hybrid clones, the gene for PEPB was assigned to mink chromosome 9.

Key words

American mink Peptidases Polymorphism Gene mapping 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Brown S, Lalley PA, Minna JD (1978) Assignment of the gene for peptidase S (PEPS) to chromosome 4 in man and confirmation of peptidase D (PEPD) assignment to chromosome 19. Human gene mapping 4. Cytogenet Cell Genet 22:167–171Google Scholar
  2. Dain AR, Tucker EM, Donker RA, Clarke SW (1984) Chromosome mapping in cattle using mouse myeloma/calf lymph node cell hybridomas. Biochem Genet 22:429–439Google Scholar
  3. Dawson WD, Huang LL, Felder MR, Schaffer JB (1983) Linkage relationships among eleven biochemical loci in Peromyscus. Biochem Genet 21:1101–1114Google Scholar
  4. Dolf GJ (1984) Genkartierung beim Schwein mit Hilfe von somalischen Zellhybriden. Diss ETH 7644, ZürichGoogle Scholar
  5. Chen TR, McMorris FA, Creagan R, Ricciuti F, Tischfield J, Ruddle FH (1973) Assignment of the gene for malate oxidoreductase decarboxylating to chromosome 6 and peptidase B and lactate dehydrogenase B to chromosome 12 in man. Am J Hum Genet 25:200–207Google Scholar
  6. Cowmeadow MP, Ruddle FH (1978) Computer-assisted statistical procedures for somatic cell gene assignment. Human gene mapping 4. Cytogenet Cell Genet 22:694–697Google Scholar
  7. Creagan R, Tischfield J, McMorris FA, Chen S, Hirschi M, Chen TR, Ricciuti F, Ruddle FH (1973) Assignment of the genes for human peptidase A to chromosome 18 and cytoplasmic glutamic oxaloacetate transaminase to chromosome 10 using somatic cell hybrids. Cytogenet Cell Genet 12:187–198Google Scholar
  8. Francke U, Lalley PA, Moss W, Ivy J, Minna JD (1977) Gene mapping in Mus musculus by interspecific cell hybridization: assignment of the genes for tripeptidase-1 to chromosome 10, dipeptidase-2 to chromosome 18, acid phosphatase-1 to chromosome 12, and adenylate kinase-1 to chromosome 2. Cytogenet Cell Genet 19:57–84Google Scholar
  9. Frick L (1983) An electrophoretic investigation of the cytosolic di- and tripeptidases of fish: molecular weights, substrate specificities, and tissue and phylogenetic distributions. Biochem Genet 21:309–322Google Scholar
  10. Harris H, Hopkinson DA (1976) Handbook of enzyme electrophoresis in human genetics. North-Holland, AmsterdamGoogle Scholar
  11. Juneja RK, Lundin LG, Gahne B (1981) Genetic polymorhism of an α1-protease inhibitor in mink plasma. Hereditas 94:249–252Google Scholar
  12. Lalley PA, Francke U, Minna JD (1978a) Comparative gene mapping: the linkage relationships of the homologous genes for phosphoglucomutase and peptidase S are conserved in man and mouse. Human gene mapping 4. Cytogenet Cell Genet 22:573–576Google Scholar
  13. Lalley PA, Francke U, Minna JD (1978b) Comparative gene mapping in man and mouse: assignment of the genes for lactate dehydrogenase-A, peptidase-D, and isocitrate dehydrogenase-2 to mouse chromosome 7. Human gene mapping 4. Cytogenet Cell Genet 22:577–580Google Scholar
  14. Leinwand LA, Kozak CA, Ruddle FH (1978) Assignment of the genes for triose phosphate isomerase to chromosome 6 and tripeptidase-1 to chromosome 10 in Mus musculus by somatic cell hybridization. Somat Cell Genet 4:233–240Google Scholar
  15. Lewis WHP, Harris H (1967) Human red cell peptidases. Nature 215:351–355Google Scholar
  16. Lewis WHP, Truslove GM (1969) Electrophoretic heterogeneity of mouse erythrocyte peptidases. Biochem Genet 3: 493–498Google Scholar
  17. Lewis WHP (1972) Genetically determined polymorphism of two peptidases in the tuco-tuco (Ctenomys talarum talarum). J Zool 166:357–362Google Scholar
  18. McAlpine PJ, Mohandas T, Roy M, Hamerton JC (1975) Assignment of pyrophosphatase gene locus (PPT) to chromosome 10 and peptidase D gene locus (PEPD) to chromosome 19 in man. Am J Hum Genet 27:61 AGoogle Scholar
  19. O'Brien SJ, Gail MH, Levin DL (1980) Correlative genetic variation in natural populations of cats, mice and men. Nature 288:580–583Google Scholar
  20. O'Brien SJ, Nash WG (1982) Genetic mapping in mammals: chromosome map of domestic cat. Science 216:257–265Google Scholar
  21. Peters J, Povey S, Jeremiah S, De Giorgi L (1983) Linkage relationships of peptidase-7, Pep-7, in the mouse. Biochem Genet 21:801–807Google Scholar
  22. Robinson R (1975) The American mink, Mustela vison. In: Handbook of genetics, vol 4. Plenum Press, NewYork London, pp 367–398Google Scholar
  23. Roderick TB, Lalley PA, Davisson MT, O'Brien SJ, Womack JE, Creau-Goldberg N, Echard G, Moore KL (1984) Report of the committee on comparative mapping. Human gene mapping 7. Cytogenet Cell Genet 37:312–339Google Scholar
  24. Rubtsov NB, Radjabli SI, Gradov AA, Serov OL (1981a) Chinese hamster × American mink somatic cell hybrids: characterization of a clone panel and assignment of the mink genes for malate dehydrogenase, NADP-1 and malate dehydrogenase, NAD-1. Theor Appl Genet 60: 99–106Google Scholar
  25. Rubtsov NB, Radjabli SI, Gradov AA, Serov OL (1981b) Chromosome localization of three syntenic gene pairs in the American mink (Mustela vison). Cytogenet Cell Genet 31:184–187Google Scholar
  26. Rubtsov NB, Gradov AA, Serov OL (1982) Chromosome localization of the loci GOT1, PP, NP, SOD1, PEP A and PEPC in the American mink (Mustela vison). Theor Appl Genet 63:331–336Google Scholar
  27. Ruddle FH, Ricciuti F, McMorris FA, Tischfield J, Creagan R, Darlington G, Chen T (1972) Somatic cell genetic assignment of peptidase C and the Rh linkage group to chromosome A-1 in man. Science 176:1429–1431Google Scholar
  28. Saison R (1971) A genetically controlled lactate dehydrogenase variant at the B locus in mink. Biochem Genet 5:27–31Google Scholar
  29. Saison R (1973) Red cell peptidase polymorphism in pigs, cattle, dogs and mink. Vox Sang 25:173–181Google Scholar
  30. Shackelford RM (1949) Six mutations affecting coat color in ranch-bred mink. Am Nat 83:49–68Google Scholar
  31. Shackelford RM, Moore WL (1954) Genetic basis of some white phenotypes in the ranch mink. J Hered 45:173–176Google Scholar
  32. Siciliano MJ, Stallings RL, Adair GM, Humphrey RM, Siciliano J (1983) Provisional assignment of TPI, GPI, and PEPD to Chinese hamster autosomes 8 and 9: a cytogenetic basis for functional haploidy an autosomal linkage group in CHO cells. Cytogenet Cell Genet 35:15–20Google Scholar
  33. Skow LC (1981) Genetic variation for prolidase (PEP-4) in the mouse maps near the gene for glucosephosphate isomerase (GPI-1) on chromosome 7. Biochem Genet 19:695–700Google Scholar
  34. Soulie J, de Grouchy J (1983) New gene assignments in the rabbit (Oryctologus cuniculus). Comparison with other species. Hum Genet 63:48–52Google Scholar
  35. Stallings RL, Siciliano MJ (1981) Confirmational, provisional, and/or regional assignment of 15 enzyme loci onto Chinese hamster autosomes 1, 2, and 3. Somat Cell Genet 7:683–698Google Scholar
  36. Wijnen LMM, Grzeschik K-H, Pearson PL, Meera Khan P (1977) The human PGM-2 and its chromosomal localization in man-mouse hybrids. Hum Genet 37:271–278Google Scholar
  37. Wilson D, Harrison B, Caron P (1984) Assignment of the human gene for peptidase E to the chromosomal region 17q23–17qter. Ann Human Genet 48:43–48Google Scholar
  38. Womack JE, Fitzgerald KB (1973) Comparative tissue distribution and substrate specificity of electrophoretical distinct peptidases in the rabbit, the rat and the gerbil. Comp Biochem Physiol B 44:115–121Google Scholar
  39. Womack JE, Lynes MA, Taylor BA (1975) Genetic variation of an intestinal leucine arylaminopeptidase (Lap-I) in the mouse and its location on chromosome 9. Biochem Genet 13:511–518Google Scholar
  40. Womack JE, Cramer DV (1980) Peptidase-3 (Pep-3), dipeptidase variant in rat homologous to mouse Pep-3 (Dip-1) and human PEP-C. Biochem Genet 18:1019–1026Google Scholar
  41. Yoshida MC (1984) GPI, LDHA, and PEPD are syntenic and assigned to rat chromosome 7. Human gene mapping 7. Cytogenet Cell Genet 37:613Google Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • M. R. Mullakandov
    • 1
  • A. A. Gradov
    • 1
  • S. M. Zakijan
    • 1
  • N. B. Rubtsov
    • 1
  • O. L. Serov
    • 1
  1. 1.Institute of Cytology and GeneticsSiberian Branch of Academy of Sciences of the USSRNovosibirskUSSR

Personalised recommendations